道地药材“邓菊”之本草考证及南阳菊花的品质评价
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摘要
目的:考证南阳菊花的本草源流,溯源其盛衰史,再通过现代分析方法对现今南阳菊花进行品质评价,阐明南阳地产菊花之优势。方法:通过考证《中华医典》及相关古文献对南阳菊花的记载,阐明南阳古代菊花的源流、应用,自古文献中揭示南阳菊花与其他菊花品种的亲缘关系;再通过高效液相色谱一测多评法,对14批菊花中的包含2015年版《中国药典》指标在内的8种化学成分进行含量测定,依据结果进行综合加权评分分析;另建立14批菊花的高效液相色谱指纹图谱,并对其进行相似度、聚类分析,以期综合评价现今南阳菊花质量,并从化学成分层面阐明南阳菊花与其他产地菊花的亲缘关系。结果:本草考证显示南阳菊花自汉朝以后流传各地,后世众多药用菊花均受南阳菊花之影响;指纹图谱的相似度、聚类分析也进一步证实南阳菊花与多种菊花在化学层面上存在亲缘关系; 2015年版《中国药典》指标检测及综合评分分析结果则显示南阳菊花品质较好,非常适宜药用。结论:南阳从古至今皆为菊花优质产区,南阳菊花非常适宜药用,值得大力推广种植。
Objective: To study the origin of Nanyang Chrysanthemi Flos materia medica and its historical origin,in order to evaluate the quality of Nanyang Chrysanthemi Flos by HPLC method,and define the advantages of Nanyang Chrysanthemi Flos with the origin of Nanyang. Method: Records of Nanyang Chrysanthemi Flos in the " Chinese Medical Code" and related ancient documents were studied to explain the origin and application. The genetic relationship between Nanyang Chrysanthemi Flos and other pieces was revealed in ancient literatures. Then8 chemical constituents in 14 batches of Chrysanthemi Flos were determined by HPLC multi-components quantitation,and the comprehensive weighted score analysis was performed based on the results. The HPLC fingerprints were established, and the similarity analysis and clustering analysis were made to comprehensive evaluation the quality of Nanyang Chrysanthemi Flos and define the genetic relationship between Nanyang and other pieces at the chemical composition level. Result: The results of the herbal textual research show that Nanyang Chrysanthemi Flos spread from place to place since the Han dynasty,and impact many medicinal chrysanthemums of later generations. HPLC fingerprints similarity and cluster analysis also indicated the genetic relationship between Nanyang Chrysanthemi Flos and other species at the chemical level. The comprehensive score analysis results show that Nanyang Chrysanthemi Flos is of good quality and very suitable for medicinal purposes. Conclusion: Nanyang has been a quality production area of Chrysanthemi Flos since ancient times to present. Nanyang Chrysanthemi Flos is very suitable for medicinal purposes and worthy of promotion.
Objective: To study the origin of Nanyang Chrysanthemi Flos materia medica and its historical origin,in order to evaluate the quality of Nanyang Chrysanthemi Flos by HPLC method,and define the advantages of Nanyang Chrysanthemi Flos with the origin of Nanyang. Method: Records of Nanyang Chrysanthemi Flos in the " Chinese Medical Code" and related ancient documents were studied to explain the origin and application. The genetic relationship between Nanyang Chrysanthemi Flos and other pieces was revealed in ancient literatures. Then8 chemical constituents in 14 batches of Chrysanthemi Flos were determined by HPLC multi-components quantitation,and the comprehensive weighted score analysis was performed based on the results. The HPLC fingerprints were established, and the similarity analysis and clustering analysis were made to comprehensive evaluation the quality of Nanyang Chrysanthemi Flos and define the genetic relationship between Nanyang and other pieces at the chemical composition level. Result: The results of the herbal textual research show that Nanyang Chrysanthemi Flos spread from place to place since the Han dynasty,and impact many medicinal chrysanthemums of later generations. HPLC fingerprints similarity and cluster analysis also indicated the genetic relationship between Nanyang Chrysanthemi Flos and other species at the chemical level. The comprehensive score analysis results show that Nanyang Chrysanthemi Flos is of good quality and very suitable for medicinal purposes. Conclusion: Nanyang has been a quality production area of Chrysanthemi Flos since ancient times to present. Nanyang Chrysanthemi Flos is very suitable for medicinal purposes and worthy of promotion.
引文
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[21]王德群,梁益敏,刘守金.中国药用菊花的品种演变[J].中国中药杂志,1999,24(10):584.
[22]HUANG L Q,GUO L P,MA C Y. Top-geoherbs of traditional Chinese medicine:common traits,quality characteristics and formation[J]. Front Med,2011,5(2):185-194.
[23]代震,利顺欣,陈随清,等.怀菊花种质资源品质评价[J].中国实验方剂学杂志,2017,23(6):48-54.
[2]王亮,汪涛,郭巧生,等.昆仑雪菊与杭菊、贡菊主要活性成分比较[J].中国中药杂志,2013,38(20):3442-3445.
[3]吴茜茜.菊花主产区种质资源遗传多样性研究[D].杭州:杭州师范大学,2016.
[4]许敬生.南阳菊潭考[J].中华医史杂志,2010,40(5):314-316.
[5]班固.汉书·地理志[M].北京:中华书局,1962:1548.
[6]应劭.王利器校注.风俗通义[M].北京:中华书局,1981:598.
[7]周密.说郛荆州[M].刻本.李际期宛委山堂,1646(顺治三年).
[8]内乡县地方史志编纂委员会.内乡县志[M].北京:生活·读书·新知三联书店,1994.
[9]龚胜生.南阳菊考[J].南都学坛,1991(2):99-102.
[10]苏颂.本草图经:草部[M].尚志钧,辑校.合肥:安徽科学技术出版社,1994:88.
[11]寇宗爽.本草衍义[M].上海:商务印书馆,1987:45.
[12]唐慎微.证类本草[M].北京:华夏出版社,1993:132.
[13]李时珍.本草纲目:草部[M].北京:人民卫生出版社,1982.
[14]汪碧涛.楚地菊花溯源[J].农业考古,2006(3):19-20.
[15]邵清松,郭巧生.药用菊花道地药材形成源流考[J].时珍国医国药,2009,20(7):1751-1752.
[16]JIN M,ZHU Z B,GUO Q S,et al. Growth and accumulation of bioactive compounds in medicinal Chrysanthemum morifolium Ramat. cv.‘Chuju’under different colored shade polyethylene[J]. J Med Plants Res,2012,6(3):398.
[17]赵玉丛,刘国际,任保增,等.菊花的HPLC指纹图谱研究[J].中草药,2005,36(10):116-119.
[18]程宏英.菊花活性成分测定与指纹图谱研究[D].无锡:江南大学,2007.
[19]刘伟,邢志霞,陈志红,等.怀菊花HPLC指纹图谱的研究[J].药物分析杂志,2007,27(8):1178-1181.
[20]张敏,朱忠华.中药菊花的名实考证[J].中国实验方剂学杂志,2018,24(6):217-223.
[21]王德群,梁益敏,刘守金.中国药用菊花的品种演变[J].中国中药杂志,1999,24(10):584.
[22]HUANG L Q,GUO L P,MA C Y. Top-geoherbs of traditional Chinese medicine:common traits,quality characteristics and formation[J]. Front Med,2011,5(2):185-194.
[23]代震,利顺欣,陈随清,等.怀菊花种质资源品质评价[J].中国实验方剂学杂志,2017,23(6):48-54.