5个圆柏优良品种(系)扦插繁殖技术研究
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摘要
为圆柏优良品种(系)的增殖扩繁和推广提供理论和技术支持,采用完全随机区组试验设计,研究了5个圆柏品种(系)、4种生长调节剂种类(ABT-1、IBA、NAA、IBA:NAA=2:1)和5种生长调节剂浓度(50、100、200、500、1 000 mg·L-1)对生根类型和生根指标的影响。结果表明,‘蓝塔’‘叠翠’‘峰桧’‘绒团’‘金花’5个圆柏品种(系)生根指标隶属函数值最高处理分别为1 000 mg·L-1 IBA:NAA=2:1速蘸10 s (0.678)、200mg·L~(-1) IBA:NAA=2:1浸泡4h(0.685)、1 000mg·L-1 IBA:NAA=2:1速蘸10 s(0.497)、100 mg·L-1 IBA:NAA=2:1浸泡4h(0.378)、500 mg·L~(-1) NAA浸泡4h(0.494);各品种(系)遗传特性差异大,其中‘叠翠’生根率最高;圆柏当年生枝条扦插以愈伤生根为主,皮部生根为辅,混合生根最少。圆柏品种(系)的生根能力受到遗传基因的控制,其扦插生根能力对生长调节剂种类和浓度的要求,有一定的特异性。
Aims to provide theoretical and technical support for the multiplication and spread of superior Sabina chinensis varieties and clones. Using a completely randomized block design, four Sabina varieties and one clone, four plant growth regulator types(ABT-1,IBA, NAA, IBA:NAA = 2:1) and five plant growth regulator concentrations(50, 100, 200, 500, 1 000 mg·L-1) were studied on rooting effects. The results showed that the highest values of membership functions of rooting indexes of the Sabina chinesis ‘Lanta', clone‘Diecui', Sabina chinesis ‘Fenggui', Sabina chinesis ‘Rongtuan' and Sabina chinesis ‘Jinhua' were 1 000 mg·L-1 IBA: NAA = 2:1 soaked for 10 s(0.678), 200 mg·L-1 IBA:NAA = 2:1 soaked for 4 h(0.685), 1 000 mg·L-1 IBA: NAA = 2:1 soaked for 10 s(0.497) and 100 mg·L-1 soaked for 4 h(0.378) and 500 mg·L-1 IBA:NAA=2:1 soaked for 4 h(0.494). The genetic characteristics of each variety or clone are different, of which clone ‘Diecui' has the highest rooting rate. The annual cuttings of Sabina chinensis were mainly rooted in callus,followed by cortex and their mixture was least. The rooting ability of Sabina chinesis is genetically controlled. The rooting capacity of different varietes or clones requires specific types and concentrations of plant growth regulator.
Aims to provide theoretical and technical support for the multiplication and spread of superior Sabina chinensis varieties and clones. Using a completely randomized block design, four Sabina varieties and one clone, four plant growth regulator types(ABT-1,IBA, NAA, IBA:NAA = 2:1) and five plant growth regulator concentrations(50, 100, 200, 500, 1 000 mg·L-1) were studied on rooting effects. The results showed that the highest values of membership functions of rooting indexes of the Sabina chinesis ‘Lanta', clone‘Diecui', Sabina chinesis ‘Fenggui', Sabina chinesis ‘Rongtuan' and Sabina chinesis ‘Jinhua' were 1 000 mg·L-1 IBA: NAA = 2:1 soaked for 10 s(0.678), 200 mg·L-1 IBA:NAA = 2:1 soaked for 4 h(0.685), 1 000 mg·L-1 IBA: NAA = 2:1 soaked for 10 s(0.497) and 100 mg·L-1 soaked for 4 h(0.378) and 500 mg·L-1 IBA:NAA=2:1 soaked for 4 h(0.494). The genetic characteristics of each variety or clone are different, of which clone ‘Diecui' has the highest rooting rate. The annual cuttings of Sabina chinensis were mainly rooted in callus,followed by cortex and their mixture was least. The rooting ability of Sabina chinesis is genetically controlled. The rooting capacity of different varietes or clones requires specific types and concentrations of plant growth regulator.
引文
[1]张颖文.沈阳桧柏的扦插育苗试验[J].中国林副特产,2004(5):3-4.
[2]王金龙.丹东桧柏半木质化嫩枝扦插技术研究[J].防护林科技,2016(3):36-37.
[3]苏国兴,洪法水.桑品种耐盐性的隶属函数法之评价[J].江苏农业学报,2002,18(1):42-47.
[4]沈海龙.苗木培育学[M].北京:中国林业出版社,2009.
[5]郭素娟.林木扦插生根的解剖学及生理学研究进展[J].北京林业大学学报,1997,19(4):64-69.
[6]李合生.现代植物生理学[M].北京:高等教育出版社,2001:217-224.
[7]齐秀东,郭守华,于凤鸣,等.NAA和IBA对金露梅扦插生根的作用[J].江苏农业科学,2007(1):103-105.
[8]何崇单,姚锐,黄瑞春,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12.
[9]赵爽,郑刚,季梦成,等.山木通扦插生根过程中内源激素的动态变化[J].江苏农业科学,2018,46(3):148-150.
[10]张锦春,刘有军,王方琳,等.沙生柽柳扦插生根过程插穗相关理化特征分析[J].西北植物学报,2018,38(3):484-492.
[11]欧阳芳群,付国赞,王军辉,等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,51(3):155-162.
[12]王艳晶,彭祚登.不同生根促进剂对国槐嫩枝扦插生根过程中内源激素变化的影响[J].西北林学院学报,2017,32(5):109-114.
[13]王远,常永,张红敏,等.IBA与NAA对罗城石楠扦插生根的影响[J].江苏林业科技,2010,37(5):30-32.
[14]韦树根,马小军,施力军,等.钩藤扦插繁殖试验[J].江苏农业科学,2011,39(6):415-416.
[15]王书胜,单文,张乐华,等.植物生长调节剂对鹿角杜鹃扦插繁殖的影响[J].植物科学学报,2014,32(2):158-167.
[16]BERHE D,NEGASH L.Asexual propagation of Juniperus procera from Ethiopia:a contribution to the conservation of African pencil cedar[J].Forest Ecology&Management,1998,112(1/2):179-190.
[17]马德滋,赵玉珑.圆柏先成不定根原基的观察[J].林业科学,1983,19(1):98-100.
[18]谢素琴.“成龄桧柏扦插生根力的研究”取得成果[J].内蒙古林业,1987(2):29.
[19]杜伟,曹旭,殷朝瑞,等.桑树硬枝扦插不同生根类型插穗的生理特性研究[J].蚕业科学,2017,43(1):18-24.
[20]李晓欣,常金宝,柴楠.对4种榆属植物嫩枝扦插生根的初步研究[J].现代园艺,2017(20):15-16.
[21]王良桂,杜旭华,王顺财,等.不同楸树品种(类型)嫩枝扦插生根能力及扦插繁殖技术[J].南京林业大学学报(自然科学版),2008,32(5):127-130.
[22]王紫阳,徐建华,李火根,等.中山杉优良无性系302,118,405扦插生根能力比较[J].浙江农林大学学报,2015,32(4):648-654.
[23]RIGAL A,YORDANOV Y S,PERRONE I,et al.The AINTEGUMENTA LIKE1 homeotic transcription factor PtAIL1controls the formation of adventitious root primordia in poplar[J].Plant Physiology,2012,160(4):1996-2006.
[24]XUAN L,XU M,CHEN C,etal.Identification and characterization of three PeSHRs,and one PeSCR,involved in adventitious root development of Populus[J].Plant Cell Tissue&Organ Culture,2014,117(2):253-264.
[25]HAN H,SUN X,XIE Y,et al.Transcriptome and proteome profiling of adventitious root development in hybrid larch(Larix kaempferi×Larix olgensis)[J].Bmc Plant Biology,2014,14(1):305.
[26]SORIN C,NEGRONI L,BALLIAU T,et al.Proteomic analysis of different mutant genotypes of Arabidopsis led to the identification of 11 proteins correlating with adventitious root development[J].Plant Physiology,2006,140(1):349-364.
[27]LI Y H,ZOU M H,FENG B H,et al.Molecular cloning and characterization of the genes encoding an auxin efflux carrier and the auxin influx carriers associated with the adventitious root formation in mango(Mangifera indica L.)cotyledon segments[J].Plant Physiology&Biochemistry,2012,55(2):33-42.
[28]RAFTOPOULOU M.PINOID pinpoints auxin[J].Nature Cell Biology,2004,6(12):1149-1149.
[2]王金龙.丹东桧柏半木质化嫩枝扦插技术研究[J].防护林科技,2016(3):36-37.
[3]苏国兴,洪法水.桑品种耐盐性的隶属函数法之评价[J].江苏农业学报,2002,18(1):42-47.
[4]沈海龙.苗木培育学[M].北京:中国林业出版社,2009.
[5]郭素娟.林木扦插生根的解剖学及生理学研究进展[J].北京林业大学学报,1997,19(4):64-69.
[6]李合生.现代植物生理学[M].北京:高等教育出版社,2001:217-224.
[7]齐秀东,郭守华,于凤鸣,等.NAA和IBA对金露梅扦插生根的作用[J].江苏农业科学,2007(1):103-105.
[8]何崇单,姚锐,黄瑞春,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12.
[9]赵爽,郑刚,季梦成,等.山木通扦插生根过程中内源激素的动态变化[J].江苏农业科学,2018,46(3):148-150.
[10]张锦春,刘有军,王方琳,等.沙生柽柳扦插生根过程插穗相关理化特征分析[J].西北植物学报,2018,38(3):484-492.
[11]欧阳芳群,付国赞,王军辉,等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,51(3):155-162.
[12]王艳晶,彭祚登.不同生根促进剂对国槐嫩枝扦插生根过程中内源激素变化的影响[J].西北林学院学报,2017,32(5):109-114.
[13]王远,常永,张红敏,等.IBA与NAA对罗城石楠扦插生根的影响[J].江苏林业科技,2010,37(5):30-32.
[14]韦树根,马小军,施力军,等.钩藤扦插繁殖试验[J].江苏农业科学,2011,39(6):415-416.
[15]王书胜,单文,张乐华,等.植物生长调节剂对鹿角杜鹃扦插繁殖的影响[J].植物科学学报,2014,32(2):158-167.
[16]BERHE D,NEGASH L.Asexual propagation of Juniperus procera from Ethiopia:a contribution to the conservation of African pencil cedar[J].Forest Ecology&Management,1998,112(1/2):179-190.
[17]马德滋,赵玉珑.圆柏先成不定根原基的观察[J].林业科学,1983,19(1):98-100.
[18]谢素琴.“成龄桧柏扦插生根力的研究”取得成果[J].内蒙古林业,1987(2):29.
[19]杜伟,曹旭,殷朝瑞,等.桑树硬枝扦插不同生根类型插穗的生理特性研究[J].蚕业科学,2017,43(1):18-24.
[20]李晓欣,常金宝,柴楠.对4种榆属植物嫩枝扦插生根的初步研究[J].现代园艺,2017(20):15-16.
[21]王良桂,杜旭华,王顺财,等.不同楸树品种(类型)嫩枝扦插生根能力及扦插繁殖技术[J].南京林业大学学报(自然科学版),2008,32(5):127-130.
[22]王紫阳,徐建华,李火根,等.中山杉优良无性系302,118,405扦插生根能力比较[J].浙江农林大学学报,2015,32(4):648-654.
[23]RIGAL A,YORDANOV Y S,PERRONE I,et al.The AINTEGUMENTA LIKE1 homeotic transcription factor PtAIL1controls the formation of adventitious root primordia in poplar[J].Plant Physiology,2012,160(4):1996-2006.
[24]XUAN L,XU M,CHEN C,etal.Identification and characterization of three PeSHRs,and one PeSCR,involved in adventitious root development of Populus[J].Plant Cell Tissue&Organ Culture,2014,117(2):253-264.
[25]HAN H,SUN X,XIE Y,et al.Transcriptome and proteome profiling of adventitious root development in hybrid larch(Larix kaempferi×Larix olgensis)[J].Bmc Plant Biology,2014,14(1):305.
[26]SORIN C,NEGRONI L,BALLIAU T,et al.Proteomic analysis of different mutant genotypes of Arabidopsis led to the identification of 11 proteins correlating with adventitious root development[J].Plant Physiology,2006,140(1):349-364.
[27]LI Y H,ZOU M H,FENG B H,et al.Molecular cloning and characterization of the genes encoding an auxin efflux carrier and the auxin influx carriers associated with the adventitious root formation in mango(Mangifera indica L.)cotyledon segments[J].Plant Physiology&Biochemistry,2012,55(2):33-42.
[28]RAFTOPOULOU M.PINOID pinpoints auxin[J].Nature Cell Biology,2004,6(12):1149-1149.