木犀草素在离子液体修饰碳糊电极上的电化学行为研究
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摘要
制备了N-丁基吡啶三氟甲磺酸盐离子液体修饰碳糊电极,研究了木犀草素在N-丁基吡啶三氟甲磺酸盐([BPy]OTF)修饰碳糊电极上的电化学行为。实验发现,在pH=3.0的柠檬酸-柠檬酸钠缓冲体系中,木犀草素在0.6221 V和0.4267 V处产生一对明显的氧化,还原峰,电流信号分别是裸电极的2.06倍,5.8倍,表明[BPy]OTF对木犀草素具有一定的催化增敏效果,木犀草素在修饰电极上的电极过程为2质子2电子转移过程,在此基础上研究了木犀草素与牛血清蛋白之间的相互作用。
The N-Butyl-Pyridine Triflate([BPy]OTF)modified ionic liquid carbon paste electrode(ILCPE)was fabricated.The electrochemical behavior of Luteolin at the ILCPE electrode was studied in detail.It is found experimentally that in pH 3.0 citric acid-sodium citrate buffer solution,Luteolin's peak potentials are 0.6221 V and 0.4267 V(vs.SCE)respectively,peak current signals are bare electrodes 2.06 and 5.8 times,The ILCPE electrode showed good catalytic activity and sensitizing effect for the Luteolin.Besides,the electrode process was double protons-double electrons reaction,which was controlled by absorption.On this basis,the interaction of Luteolin with BSA were studied.
The N-Butyl-Pyridine Triflate([BPy]OTF)modified ionic liquid carbon paste electrode(ILCPE)was fabricated.The electrochemical behavior of Luteolin at the ILCPE electrode was studied in detail.It is found experimentally that in pH 3.0 citric acid-sodium citrate buffer solution,Luteolin's peak potentials are 0.6221 V and 0.4267 V(vs.SCE)respectively,peak current signals are bare electrodes 2.06 and 5.8 times,The ILCPE electrode showed good catalytic activity and sensitizing effect for the Luteolin.Besides,the electrode process was double protons-double electrons reaction,which was controlled by absorption.On this basis,the interaction of Luteolin with BSA were studied.
引文
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[3]王继双,何焱,张文静,等.木犀草素的药理作用研究进展[J].生命科学,2013,25(6):560-565.
[4]王启钦,程宁,阳琼芳,等.一锅法合成新型木犀草素衍生物[J].化学研究与应用,2012,24(12):1831-1835.
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[6]Musameh M M,Kachoosangi R T,Compton R G.Ionic liquid-carbon composite glucose biosensor[J].Anal Chem,2008,133(1):133-138.
[7]顾玲,柯苗,闫璟,等.抗坏血酸在钴酞菁修饰碳糊电极上的电化学行为[J].化学研究与应用,2017,29(2):173-177.
[8]Zhu Y H,Zhang Z L,Pang D W.Electrochemical oxidation of theophylline at multi-wall carbon nanotube modified glassy carbon electrodes[J].J Electroanal Chem,2005,581(2):303-309.
[9]李玲,王娟,刘计敏,等.L-半胱氨酸/纳米金/DNA/壳聚糖修饰金电极测定布洛芬[J].分析科学学报,2016,32(5):644-648.