不同分子质量的大豆肽螯合钙工艺优化
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摘要
利用高压脉冲电场处理大豆肽螯合钙,探究不同分子质量的大豆肽与钙离子螯合的效果。以不同分子质量的大豆肽为主要原料,以螯合率为衡量指标,研究大豆肽与钙离子的质量比,反应体系的p H以及电场处理过程中电场强度、电场频率4个因素对螯合率的影响。利用响应面试验设计的方法对试验结果进行分析。经验证,分子质量为3~10 ku的大豆肽与钙离子螯合效果最佳,其回归方程为Y=96.35-0.052X_1+0.43X2-0.24X_3-0.49X_4-0.24X_1X2+0.025X_1X_3+0.21X_1X_4-0.11X2X_3+0.69X2X_4-0.13X_3X_4-0.55X_12-0.88X22-0.73X_32-0.60X_42。优化的大豆肽螯合钙的最佳工艺条件:大豆肽与钙质量比1∶1.95,p H 8,电场强度15 k V/cm,电场频率1 200 Hz。在此条件下得到的螯合率为(97.52±0.12)%。本试验为生物活性钙的生产工艺研究提供一种新思路。
In our study, soybean peptides of various molecular weight were treated with pulsed electric field(PEF)to investigate the calcium-chelating activity. We adopted soybean peptides of various molecular weight as the raw material, chelating rate was used to evaluate the effect of the pulsed electric field(PEF) treatment parameters on it. Four factors were examined to optimize the effect of pulsed electric field treatment parameters on chelating rate of soybean peptides by one-factor-at-a-time and response surface experiment, the result indicated that 3-10 ku soybean protein hydrolysates chelating calcium showed the highest chelating rate, and the regression model was: Y=96.35-0.052X_1+0.43X_2-0.24X_3-0.49X_4-0.24X_1X_2+0.025X_1X_3+0.21X_1X_4-0.11X_2X_3+0.69X_2X_4-0.13X_3X_4-0.55X_12-0.88X_22-0.73X_32-0.60X_42. Optimal conditions were as follows: concentration rate was 1 ∶1.95, p H of the reaction system were 8, electric field intensity 15 k V/cm, pulse frequency 1 200 Hz, under which, the chelating rate increased to 97.52% ± 0.12%, this research provides a new idea for the production of bioactive calcium.
In our study, soybean peptides of various molecular weight were treated with pulsed electric field(PEF)to investigate the calcium-chelating activity. We adopted soybean peptides of various molecular weight as the raw material, chelating rate was used to evaluate the effect of the pulsed electric field(PEF) treatment parameters on it. Four factors were examined to optimize the effect of pulsed electric field treatment parameters on chelating rate of soybean peptides by one-factor-at-a-time and response surface experiment, the result indicated that 3-10 ku soybean protein hydrolysates chelating calcium showed the highest chelating rate, and the regression model was: Y=96.35-0.052X_1+0.43X_2-0.24X_3-0.49X_4-0.24X_1X_2+0.025X_1X_3+0.21X_1X_4-0.11X_2X_3+0.69X_2X_4-0.13X_3X_4-0.55X_12-0.88X_22-0.73X_32-0.60X_42. Optimal conditions were as follows: concentration rate was 1 ∶1.95, p H of the reaction system were 8, electric field intensity 15 k V/cm, pulse frequency 1 200 Hz, under which, the chelating rate increased to 97.52% ± 0.12%, this research provides a new idea for the production of bioactive calcium.
引文
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[12]ZHAO W,YANG R J,LU R R,et al.Effect of PEF on microbial inactivation and physical-chemical properties of green tea extracts[J].LWT-Food Science and Technology,2008,41(3):425-431.
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[2]YU Y D,ZHANG M D,LIN S Y,et al.Assessment the levels of tartrate-resistant acid phosphatase(TRAP)on mice fed with eggshell calcium citrate malate[J].Int J Biol Macroml,2013,58:253-257.
[3]李建杰,叶磊,荣瑞芬.生物活性肽的酶法制备及分离鉴定研究进展[J].食品研究与开发,2012,2(33):195-199.
[4]张连慧,贺寅,刘新旗.大豆肽制备研究进展及其在食品中的应用[J].食品工业科技,2012,24(33):423-429.
[5]李文,陈复生,丁长河,等.大豆肽生理功能的研究进展[J].食品工业科技,2013,4(34):360-367.
[6]吴蕾.胶原蛋白肽螯合钙的制备研究[D].武汉:武汉工业学院,2012.
[7]BASS J K,CHAN G M.Calcium nutrition and metabolism during infancy[J].Nutrition,2006,22:1057-1066.
[8]成静,陈栋梁,倪萌,等.胶原肽螯合钙的吸收试验研究[J].食品科技,2012,5(37):64-67.
[9]薛荣涛,李翠芹,何腊平.复合氨基酸螯合钙的研究进展[J].食品工业科技,2014,21(35):390-394.
[10]韦汉昌,韦群兰,韦善清.高压脉冲电场提取猪皮胶原蛋白[J].广西科学,2011,18(3):235-237.
[11]张若兵,陈杰,肖健夫,等.高压脉冲电场设备及其在食品非热处理中的应用[J].高电压技术,2011,3(37):777-786.
[12]ZHAO W,YANG R J,LU R R,et al.Effect of PEF on microbial inactivation and physical-chemical properties of green tea extracts[J].LWT-Food Science and Technology,2008,41(3):425-431.
[13]金声琅,王莹,殷涌光.高压脉冲电场对淀粉和肌原纤维蛋白混合凝胶强度的影响[J].吉林大学学报:工学版,2014,2(44):573-578.
[14]于倩,曾新安.高强脉冲电场强化甘氨酸铜螯合反应[J].食品与发酵工艺,2013,10(39):69-72.
[15]赫桂丹.PEF下牛骨钙离子化及胶原多肽提取优化综合开发研究[D].长春:吉林大学,2009.