超高效液相色谱–串联质谱法测定卷烟中果糖、葡萄糖、蔗糖、麦芽糖
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摘要
建立超高效液相色谱–串联质谱法测定卷烟中果糖、葡萄糖、蔗糖、麦芽糖4种水溶性糖的含量。样品用水超声提取,稀释过滤后直接进样,以Acquity BEH Amide色谱柱为分析柱,乙腈和水为流动相,梯度洗脱,采用负离子多反应监测模式分析。果糖和葡萄糖的线性范围为2.5～30μg/mL,蔗糖和麦芽糖的线性范围为0.25～3μg/mL,线性相关系数均大于0.997,方法检出限均为0.03 mg/g。加标样品的平均回收率为93.6%～101.0%,测定结果的相对标准偏差为0.7%～2.9%(n=6)。该方法简便、快速,可用于卷烟样品中果糖、葡萄糖、蔗糖、麦芽糖的测定。
A method for the determination of fructose, glucose, sucrose and maltose in cigarettes by ultra high performance liquid chromatography–tandem mass spectrometry was established. The samples were extracted by ultrasound with water, diluted and ?ltered, and then injected directly. Acquity BEH Amide column was used as analytical column,acetonitrile and water were used as mobile phase, gradient elution was used. Determination of four water–soluble sugars in samples by anion multi–reaction monitoring model. The linear range of fructose and glucose was 2.5–30 μg/mL,and that of sucrose and maltose was 0.25–3 μg/mL. The linear correlation coef?cients were more than 0.997, and the detection limits were 0.03 mg/g. The average recoveries were 93.6%–101.0%, and the relative standard deviations of the results were 0.7%–2.9%(n=6). The method is simple and rapid, and it can be used for the determination of fructose, glucose,sucrose and maltose in cigarette samples.
A method for the determination of fructose, glucose, sucrose and maltose in cigarettes by ultra high performance liquid chromatography–tandem mass spectrometry was established. The samples were extracted by ultrasound with water, diluted and ?ltered, and then injected directly. Acquity BEH Amide column was used as analytical column,acetonitrile and water were used as mobile phase, gradient elution was used. Determination of four water–soluble sugars in samples by anion multi–reaction monitoring model. The linear range of fructose and glucose was 2.5–30 μg/mL,and that of sucrose and maltose was 0.25–3 μg/mL. The linear correlation coef?cients were more than 0.997, and the detection limits were 0.03 mg/g. The average recoveries were 93.6%–101.0%, and the relative standard deviations of the results were 0.7%–2.9%(n=6). The method is simple and rapid, and it can be used for the determination of fructose, glucose,sucrose and maltose in cigarette samples.
引文
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[14]蔡凯,向章敏,张婕,等.气相色谱法同时测定初烤烟叶中的4种水溶性糖[J].分析试验室,2012,31(1):91-94.
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[16]黄翼飞.高效液相色谱法测定烟草中的果糖、葡萄糖和蔗糖[J].烟草科技,2010,43(1):40-43.
[17]黄菲,黄翼飞.高效液相色谱法测定烟草及烟草制品中的三种水溶性糖[J].中国烟草科学,2012,33(2):47-50.
[18]程勇,李庆廷,李剑,等.Prevail糖柱-HPLC-ELSD法测定烟草中水溶性糖[J].烟草科技,2010,43(3):32-35.
[19]汤建国,袁大林,乔丹娜.超高效液相色谱法测定卷烟烟丝中7种水溶性糖含量[J].化学分析计量,2012,21(1):17-19.
[20]孔兰芬,杨式华,杨玉标,等.UPLC-ELSD法测定烟草中的水溶性糖[J].云南化工,2012,39(3):35-37.
[21]毛智慧,汤建国,杨继,等.烟草中水溶性糖的超高效液相色谱分析及聚类分析研究[J].分析试验室,2011,30(3):115-118.
[22]赵瑜,刘金云,汤威,等.高效液相色谱-串联质谱法测定烟草中的七种糖类物质[J].食品工业科技,2012,33(12):83-86.
[23]王川丕,诸力,刘新,等.超高效液相色谱-质谱联用技术测定茶叶中8种单、寡糖的含量[J].食品科学,2014,35(20):164-166.
[2]云南省烟草科学研究所.云南烟草栽培学[M].北京:科学出版社,2007:60.
[3]夏体渊,王炽,陈兴业,等.不同烤烟品种留叶数对烤烟产量、化学成分、评吸质量的影响[J].西南农业学报,2017,30(3):681-683.
[4]杜坚,王珂清,张建强,等.我国不同生态区云烟87烟叶主要化学成分及感官风格差异[J].中国烟草科学,2018,39(2):96-99.
[5]时鹏,申国明,向德恩,等.恩施烟区主要气候因子与烤烟烟叶化学成分的关系[J].中国烟草科学,2012,33(4):13-16.
[6]姬兴杰,孟寒冬,左璇,等.河南烟区主要气候因子与烤烟烟叶化学成分的关系[J].中国烟草科学,2017,38(1):35-38.
[7]陈颐,杨佳玫,王玉平,等.不同仓贮环境对片烟可溶性糖及生物碱含量的影响[J].西南农业学报,2017,30(5):1 020-1 023.
[8]宋鹏飞,王萝萍,钱颖颖,等.不同产地、品种、仓储地点烤烟片烟陈化中化学成分的变化[J].西南农业学报,2018,31(3):488-491.
[9]夏冰冰,梁永江,张扬,等.遵义烟区上部烟叶化学成分与感官评吸的相关性[J].中国烟草科学,2015,36(1):30-33.
[10]林顺顺,张晓鸣.基于PLSR分析烟叶化学成分与感官质量的相关性[J].中国烟草科学,2016,37(1):78-81.
[11]吴春,王轶,蒲文宣,等.中间香型烟叶特色彰显度与主要化学成分的相关及通径分析[J].中国烟草科学,2012,33(4):1-4.
[12]常乃杰,张云贵,刘青丽,等.应用SPSS软件研究典型中间香型烤烟常规化学成分与烟气成分的关系[J].烟草科技,2017,50(4):31-34.
[13]姜振玲,于卫松,孙建,等.离子色谱法同时测定烟草中6种糖[J].理化检验(化学分册),2015,51(9):39-42.
[14]蔡凯,向章敏,张婕,等.气相色谱法同时测定初烤烟叶中的4种水溶性糖[J].分析试验室,2012,31(1):91-94.
[15]张峻松,宣晓泉,唐纲岭,等.毛细管气相色谱法测定烟草中葡萄糖、果糖、蔗糖的含量[J].中国烟草学报,2007,13(2):17-19.
[16]黄翼飞.高效液相色谱法测定烟草中的果糖、葡萄糖和蔗糖[J].烟草科技,2010,43(1):40-43.
[17]黄菲,黄翼飞.高效液相色谱法测定烟草及烟草制品中的三种水溶性糖[J].中国烟草科学,2012,33(2):47-50.
[18]程勇,李庆廷,李剑,等.Prevail糖柱-HPLC-ELSD法测定烟草中水溶性糖[J].烟草科技,2010,43(3):32-35.
[19]汤建国,袁大林,乔丹娜.超高效液相色谱法测定卷烟烟丝中7种水溶性糖含量[J].化学分析计量,2012,21(1):17-19.
[20]孔兰芬,杨式华,杨玉标,等.UPLC-ELSD法测定烟草中的水溶性糖[J].云南化工,2012,39(3):35-37.
[21]毛智慧,汤建国,杨继,等.烟草中水溶性糖的超高效液相色谱分析及聚类分析研究[J].分析试验室,2011,30(3):115-118.
[22]赵瑜,刘金云,汤威,等.高效液相色谱-串联质谱法测定烟草中的七种糖类物质[J].食品工业科技,2012,33(12):83-86.
[23]王川丕,诸力,刘新,等.超高效液相色谱-质谱联用技术测定茶叶中8种单、寡糖的含量[J].食品科学,2014,35(20):164-166.