丙型肝炎患者血清IL-17与ALT等指标相关性分析
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摘要
目的了解慢性丙型肝炎患者和对照组血清ALT、AST、TBil、IL-17水平以及在丙型肝炎的不同发展阶段的变化特点,探讨病毒载量与IL-17、ALT、AST、TBil的相关性。方法 85例丙型肝炎患者其中包括24例丙型肝炎肝硬化患者和61例慢性丙型肝炎患者,30名健康人作为对照。所有丙型肝炎患者抗-HCV抗体检测S/CO>3.8(排除抗-HCV假阳性患者),研究对象分为4组。采用全自动生化分析仪检测所有研究对象的ALT、AST、TBil的水平,采用酶联免疫吸附实验-双抗体夹心法(ELISA)检测所有研究对象血清中抗-HCV抗体和细胞因子IL-17水平。采用荧光定量聚合酶链反应(RT-PCR)检测HCV-RNA的病毒载量。结果(1)通过各组间多重比较,丙型肝炎患者各组IL-17水平显著高于健康对照组(P<0.05),差异有统计学意义,在病毒载量>1×103组IL-17水平与ALT、AST水平变化有很好的相关性。(2)按照病毒载量HCV-RNA高低分组,病毒载量与IL-17水平呈正相关,随着病毒载量的升高IL-17水平也随之升高。结论患者组IL-17、ALT、AST、TBil的检测水平升高可能与患者肝细胞的炎性损伤有关,并且IL-17在一定程度上能够反映肝脏的损伤程度。在病毒检测阳性的患者中病毒载量与IL-17水平呈正相关。
Objective The understand the characteristics of changes of ALT,AST,T-Bil and IL-17 in different stages of hepatitis C disease development. Methods Eighty-five patients with hepatitis C infection(24cases of hepatitis C cirrhosis and 61 cases of chronic hepatitis C infection)were investigated and 30 healthy persons were as controls.The automatic biochemical analyzer was used to test the ALT,AST and T-Bil levels and the ELISA method was used to test the serum anti-HCV antibody and cytokine IL-17 levels.The RT-PCR was used to test the HCV-RNA virus load. Results The IL-17 levels of all hepatitis C patients were significantly higher than that of the healthy control group(P<0.05).In the group of patients with more than 1×103virus load,the IL-17 level had a good correlation with ALT,AST and T-Bil levels.The virus load was positive correlation with the IL-17 level and the IL-17 level increased with the increasing of the virus load. Conclusions The increasing of IL-17,ALT,AST and T-Bil levels may be related to the hepatic cell inflammatory injury of patients and can reflect the liver injury degree in some extent.
Objective The understand the characteristics of changes of ALT,AST,T-Bil and IL-17 in different stages of hepatitis C disease development. Methods Eighty-five patients with hepatitis C infection(24cases of hepatitis C cirrhosis and 61 cases of chronic hepatitis C infection)were investigated and 30 healthy persons were as controls.The automatic biochemical analyzer was used to test the ALT,AST and T-Bil levels and the ELISA method was used to test the serum anti-HCV antibody and cytokine IL-17 levels.The RT-PCR was used to test the HCV-RNA virus load. Results The IL-17 levels of all hepatitis C patients were significantly higher than that of the healthy control group(P<0.05).In the group of patients with more than 1×103virus load,the IL-17 level had a good correlation with ALT,AST and T-Bil levels.The virus load was positive correlation with the IL-17 level and the IL-17 level increased with the increasing of the virus load. Conclusions The increasing of IL-17,ALT,AST and T-Bil levels may be related to the hepatic cell inflammatory injury of patients and can reflect the liver injury degree in some extent.
引文
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[12]张琳,陈巍,张丹,等.HCV感染不同阶段血清免疫细胞因子水平的动态变化[J].肝脏,2011,16(3):224-227.
[2]刘芳,张跃新,鲁小擘,等.HLA、细胞因子与丙型肝炎发展的相关性研究进展[J].中国病原生物学杂志,2012,(6):473-475.
[3]Sun J,Li K,Shata MT,et al.The immunologic basis for hepatitis C infection[J].Curr Opin Gastroenterol,2004,20(6):598-602.
[4]秦来英,杜文军,刘葵花,等.白细胞介素17的肝内表达与慢性乙型肝炎肝纤维化的相关性[J].中华肝脏病杂志,2009,17(3):221-222.
[5]Zhang JY,Zhang Z,Lin F,et al.Interleukin-17-producing CD4(+)T cells increase with severity of liver damage in patients with chronic hepatitis B[J].Hepatology,2010,51(1):81-91.
[6]中华医学会肝病分会,传染病与寄生虫病学分会.丙型肝炎防治指南[J].肝脏,2004,9(1):附页1-6.
[7]曹振环,邹梦娜,陈新月.丙型肝炎病毒感染致肝硬化的影响因素分析[J].中华实验和临床感染病杂志,2009,3(1):38-43.
[8]何长辉.丙型肝炎病毒检测方法的应用评价[J].临床和实验医学杂志,20105,9(10):753.755.
[9]甸子芩,沈云松.抗-HCV与HCV-RNA和ALT之间的关系探讨[J].中国医药指南,2012,10(27):356-359.
[10]张琳,苗亮,韩峰,等.慢性丙型肝炎患者血清细胞因子水平的变化及意义[J].细胞与分子免疫学杂志,2011,27(3):301-303.
[11]陈勇,耿健,刘文馨,等.丙型肝炎患者丙型肝炎病毒含量与白细胞介素-2和6血清水平的临床观察[J].中国药物与临床,2012,12(12):1572-1573.
[12]张琳,陈巍,张丹,等.HCV感染不同阶段血清免疫细胞因子水平的动态变化[J].肝脏,2011,16(3):224-227.