天津地区无偿献血者HCVRNA与抗-HCV、ALT检测结果相关性分析
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摘要
目的探讨本中心无偿献血者丙型肝炎病毒RNA(HCV RNA),与丙型肝炎抗体(抗-HCV)和丙氨酸氨基转移酶(ALT)检测结果之间的相关性。方法经ELISA检测抗-HCV阳性标本313例,采用转录介导的扩增(TMA)-化学发光法定性检测HCV RNA,速率法检测ALT水平。结果 313例抗-HCV阳性血清中HCV RNA阳性者141例(阳性率45.05%);抗-HCV S/CO值1-3.79实验组,HCV RNA阳性率为5%,抗-HCV S/CO值为3.80-4.99的实验组,HCV RNA阳性率为76.74%,S/CO值≥5时,HCV RNA阳性率为56.25%。各组之间差异具统计学意义(P<0.05);抗-HCV(+)/HCV RNA(-)组与抗-HCV(+)/HCV RNA(+)组的ALT检测异常率,分别为17.44%和17.73%,2组之间差异无统计学意义。结论 HCV RNA阳性率与抗-HCV的S/CO值有一定相关性;在抗-HCV异常的无偿献血者人群中,ALT异常率与HCV RNA检测结果无相关性。
Objective To investigate the correlation between HCV-RNA and anti-HCV from ALT detecftion resulfs of volunteer blood donors.Methods TMA-chemiluminescence method was employed to detect HCV-RNA and kinetic method was used to assay ALT levels in 313 anti-HCV positive samples which were tested by ELISA.Results In 313 cases positive for anti-HCV,141 cases(45.05 %) were positive for HCV-RNA.The HCV-RNA positive rates of anti-HCV in S/ CO ratio1- 3.79 group and 3.80- 4.99 group were 5% and 76.74%,respectively.The HCV-RNA positive rate of anti-HCV S/ CO ratio ≥ 5.00 group was 56.25%.The statistical difference between the groups was significant(P<0.05).The ALT abnormality rates of anti-HCV(+)/ HCV-RNA(-) group and anti-HCV(+)/ HCV-RNA(+) group were 17.44% and17.73%,respectively.The difference between the two groups was not statistically significant.Conclusion The positive rate of HCV-RNA had some correlation with anti-HCV S/ CO ratio.No correlation was observed between ALT abnormality rates and HCV-RNA in anti-HCV positive volunteer blood donors.
Objective To investigate the correlation between HCV-RNA and anti-HCV from ALT detecftion resulfs of volunteer blood donors.Methods TMA-chemiluminescence method was employed to detect HCV-RNA and kinetic method was used to assay ALT levels in 313 anti-HCV positive samples which were tested by ELISA.Results In 313 cases positive for anti-HCV,141 cases(45.05 %) were positive for HCV-RNA.The HCV-RNA positive rates of anti-HCV in S/ CO ratio1- 3.79 group and 3.80- 4.99 group were 5% and 76.74%,respectively.The HCV-RNA positive rate of anti-HCV S/ CO ratio ≥ 5.00 group was 56.25%.The statistical difference between the groups was significant(P<0.05).The ALT abnormality rates of anti-HCV(+)/ HCV-RNA(-) group and anti-HCV(+)/ HCV-RNA(+) group were 17.44% and17.73%,respectively.The difference between the two groups was not statistically significant.Conclusion The positive rate of HCV-RNA had some correlation with anti-HCV S/ CO ratio.No correlation was observed between ALT abnormality rates and HCV-RNA in anti-HCV positive volunteer blood donors.
引文
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[11]周艳,李靖,冯卓,等.结合核酸检测探讨ALT与HBV,HCV的相关性.中国输血杂志,2013,26(1):51-52.
[12]邵芳.HCV抗原,HCV抗体及HCV-RNA联合检测ALT的相关性及其临床意义.中国中西医结合消化杂志,2014,22(2):80-82.
[2]Shepard CW,Finellin L,Alter MJ.et al.Global epidemilolgy of hepatitis C virus infection.Lancent Infect Dis,2005,5(9):558-567.
[3]宋雪珍,宋雪梅,刘晓丹,等.HCV-RNA和抗-HCV的联合检测在丙型肝炎确诊中的临床意义.中国卫生检验杂志,2008,18(10):2041-2042.
[4]Meng Q,Wong C,Rangachari A,et al.Automate multiplex assay system for simultaneous detection of hepatitis B virus DNA,hepatitis virus RNA,and human immunodeficiency virus type 1RNA.J Clin Microbiol,2001,39(8):2937-2945.
[5]梁华钦,王敏,黎世杰,等.广州市无偿献血者中抗-HCV阳性人群的比较分析.中国输血杂志,2012,25(5):449-451.
[6]李金明.感染性疾病血清学检验中应重视对弱反应性标本的确认.中华检验医学杂志,2006,29(7):577-580.
[7]林洪铿,将伟梅,周晓真,等.献血者ELISA抗-HCV反应性强度与RIBA确证阳性的相关性研究.中国输血杂志,2010,23(8):617-619.
[8]El-Shamy A,Shoji I,Kim SR,et al.Sequence heterogeneity in NS5A of hepatitis C virus genotypes 2a and 2b and clinical outcome of pegylated-interferon/ribavirin therapy.PLOS ONE,2012,7(2):e30513-30513.
[9]甸子芩,沈云松.抗-HCV与HCV-RNA和ALT之间的关系探讨.中国医药指南,2012,(27):44-45.
[10]熊华平,戎霞,黄珂,等.广州地区HCV阳性无偿献血者ALT检测结果分析.中国输血杂志,2013,26(6):538-540.
[11]周艳,李靖,冯卓,等.结合核酸检测探讨ALT与HBV,HCV的相关性.中国输血杂志,2013,26(1):51-52.
[12]邵芳.HCV抗原,HCV抗体及HCV-RNA联合检测ALT的相关性及其临床意义.中国中西医结合消化杂志,2014,22(2):80-82.