水稻恶苗病菌田间抗咪鲜胺菌株的适合度及其交互抗性
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摘要
恶苗病是水稻生产上较为严重的种传真菌病害,咪唑类广谱内吸性杀菌剂咪鲜胺是目前防治该病害的主要药剂。以对咪鲜胺抗性及敏感的田间水稻恶苗病菌为试材,研究了其适合度及对几种常用杀菌剂的交互抗性。结果显示:抗性菌株的抗药性可稳定遗传,其温度敏感性与敏感菌株无明显差异,部分抗性菌株在菌丝生长速率、产孢量、孢子萌发率和致病力方面显著高于田间敏感菌株;咪鲜胺与三唑类及2-氰基丙烯酸酯类杀菌剂之间均无交互抗性。研究表明,对咪鲜胺产生抗性的水稻恶苗病菌具有较强的适合度,在田间自然条件下有可能形成优势群体,因此需合理轮换使用不同作用机制的杀菌剂,以延缓其抗药性的发展。
Bakanae disease is the most notorious seed-borne fungus disease affecting rice. Prochloraz,an imidazole fungicide with broad-spectrum and systemic activity, is the most widely used chemical for the control of this disease at present. Prochloraz-resistant and prochloraz-sensitive strains of Fusarium fujikuroi collected from fields were used in this study to characterize their fitness and cross-resistance between prochloraz and other fungicides. The results showed that most of the resistant strains could be inherited stably. No difference was observed between the resistant and sensitive strains in terms of temperature sensitivity. With regards to mycelial growth rate, conidium production, conidium germination rate and pathogenicity, some of the resistant strains were significantly higher than those of the sensitive ones. There is no cross-resistance found among prochloraz, triazoles and 2-cyanoacrylate fungicides. This study showed that some field F. fujikuroi strains with resistance to prochloraz had astrong fitness, which might develop into dominant population in the fields. In order to slow down the development of fungicide resistance, fungicides with different modes of action should be alternately applied in the field.
Bakanae disease is the most notorious seed-borne fungus disease affecting rice. Prochloraz,an imidazole fungicide with broad-spectrum and systemic activity, is the most widely used chemical for the control of this disease at present. Prochloraz-resistant and prochloraz-sensitive strains of Fusarium fujikuroi collected from fields were used in this study to characterize their fitness and cross-resistance between prochloraz and other fungicides. The results showed that most of the resistant strains could be inherited stably. No difference was observed between the resistant and sensitive strains in terms of temperature sensitivity. With regards to mycelial growth rate, conidium production, conidium germination rate and pathogenicity, some of the resistant strains were significantly higher than those of the sensitive ones. There is no cross-resistance found among prochloraz, triazoles and 2-cyanoacrylate fungicides. This study showed that some field F. fujikuroi strains with resistance to prochloraz had astrong fitness, which might develop into dominant population in the fields. In order to slow down the development of fungicide resistance, fungicides with different modes of action should be alternately applied in the field.
引文
[1]HWANG I S,AHN I P.Multi-homologous recombination-based gene manipulation in the rice pathogen Fusarium fujikuroi[J].Plant Pathol J,2016,32(3):173-181.
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[12]郝蓉蓉,郭海东,杨成德,等.西藏设施辣椒根腐病病原的分离及鉴定[J].西北农业学报,2015,24(12):139-143.HAO R R,GUO H D,YANG C D,et al.Isolation and detection of the pathogen caused capsicum root rot in Tibet[J].Acta Agriculturae Boreali-Occidentalis Sinica,2015,24(12):139-143.
[13]张晓,张艳军,陈雨,等.嘧菌酯对番茄早疫病菌的抑制作用[J].农药学学报,2008,10(1):41-46.ZHANG X,ZHANG Y J,CHEN Y,et al.Inhibitory effect of azoxystrobin on Alternaria solani[J].Chin J Pestic Sci,2008,10(1):41-46.
[14]黄彰欣.植物化学保护实验指导[M].北京:农业出版社,1993:103.HUANG Z X.Experimental guidance of plant chemical protection[M].Beijing:Agriculture Press,1993:103.
[15]赵志华,张锡明,范洁茹,等.咪鲜胺对水稻恶苗病菌及其抗药突变体生长发育的影响[J].农药学学报,2007,9(3):251-256.ZHAO Z H,ZHANG X M,FAN J R,et al.Effect of prochloraz on growth and development of prochloraz-sensitive strain and its resistant mutants in Fusarium fujikuroi[J].Chin J Pestic Sci,2007,9(3):251-256.
[16]LU X H,ZHU S S,BI Y,et al.Baseline sensitivity and resistance-risk assessment of Phytophthora capsici to iprovalicarb[J].Phytopathology,2010,100(11):1162-1168.
[17]ZHOU Y X,CHEN L,HU J,et al.Baseline sensitivity of natural population and resistance risk of Peronophythora litchii to four novel QoI fungicides[J].Eur J Plant Pathol,2016,146(1):71-83.
[18]邓维萍,杨敏,杜飞,等.葡萄胶孢炭疽菌对3种麦角甾醇脱甲基抑制剂类杀菌剂的敏感性[J].农药学学报,2011,13(3):245-252.DENG W P,YANG M,DU F,et al.Sensitivity of Colletotrichum gloeosporioides causing grape anthracnose to three sterol demethylation inhibitor(DMI)fungicides[J].Chin J Pestic Sci,2011,13(3):245-252.
[2]WULFF E G,SORENSEN J L,LUBECK M,et al.Fusarium spp.associated with rice bakanae:ecology,genetic diversity,pathogenicity and toxigenicity[J].Environ Microbiol,2010,12(3):649-657.
[3]JESTOI M.Emerging fusarium-mycotoxins fusaproliferin,beauvericin,enniatins,and moniliformin-a review[J].Crit Rev Food Sci Nutr,2008,48(1):21-49.
[4]陈平,柳训才.咪鲜胺的应用概况及其残留检测研究[J].湖北农业科学,2007,46(3):478-480.CHEN P,LIU X C.Progress on application and residue detection of the prochloraz[J].Hubei Agric Sci,2007,46(3):478-480.
[5]郑睿,聂亚锋,于俊杰,等,江苏省水稻恶苗病菌对咪鲜胺和氰烯菌酯的敏感性[J].农药学学报,2014,16(6):693-698.ZHENG R,NIE Y F,YU J J,et al.Sensitivity of Fusarium fujikuroi to prochloraz and JS399-19 in Jiangsu province[J].Chin J Pestic Sci,2014,16(6):693-698.
[6]陈夕军,卢国新,童蕴慧,等.水稻恶苗病菌对三种浸种剂的抗性及抗药菌株的竞争力[J].植物保护学报,2007,34(4):425-430.CHEN X J,LU G X,TONG Y H,et al.Resistance of Fusarium moniliforme rice bakanae disease pathogen to 3 fungicides for seedtreatment and competition ability of resistant strains[J].Acta Phytophylacica Sinica,2007,34(4):425-430.
[7]徐瑶,水稻恶苗病菌对咪鲜胺敏感性分析与药剂防治研究[D].大庆:黑龙江八一农垦大学,2015.XU Y.Research on Fusarium moniliforme to prochloraz sensitivity analysis and chemical control[D].Daqing:Heilongjiang Bayi Agricultural University,2015.
[8]SHIN M U,KANG H J,LEE Y,et al.Detection of the resistance of Fusarium spp.isolated from rice seeds to prochloraz and crossresistance to other fungicides inhibiting sterol biosynthesis[J].Kor J Pestic Sci,2008,12(3):277-282.
[9]LEE Y H,KIM S Y,CHOI H W,et al.Fungicide resistance of Fusarium fujikuroi isolated in Korea[J].Kor J Pestic Sci,2010,14(4):427-432.
[10]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998:122-124.FANG Z D.Plant disease research methods[M].3rd ed.Beijing:China Agriculture Press,1998:122-124.
[11]GEISER D M,JIMENEZ-GASCO M D M,KANG S,et al.A DNA sequence database for identifying Fusarium[J].Eur J Plant Pathol,2004,110(5):473-479.
[12]郝蓉蓉,郭海东,杨成德,等.西藏设施辣椒根腐病病原的分离及鉴定[J].西北农业学报,2015,24(12):139-143.HAO R R,GUO H D,YANG C D,et al.Isolation and detection of the pathogen caused capsicum root rot in Tibet[J].Acta Agriculturae Boreali-Occidentalis Sinica,2015,24(12):139-143.
[13]张晓,张艳军,陈雨,等.嘧菌酯对番茄早疫病菌的抑制作用[J].农药学学报,2008,10(1):41-46.ZHANG X,ZHANG Y J,CHEN Y,et al.Inhibitory effect of azoxystrobin on Alternaria solani[J].Chin J Pestic Sci,2008,10(1):41-46.
[14]黄彰欣.植物化学保护实验指导[M].北京:农业出版社,1993:103.HUANG Z X.Experimental guidance of plant chemical protection[M].Beijing:Agriculture Press,1993:103.
[15]赵志华,张锡明,范洁茹,等.咪鲜胺对水稻恶苗病菌及其抗药突变体生长发育的影响[J].农药学学报,2007,9(3):251-256.ZHAO Z H,ZHANG X M,FAN J R,et al.Effect of prochloraz on growth and development of prochloraz-sensitive strain and its resistant mutants in Fusarium fujikuroi[J].Chin J Pestic Sci,2007,9(3):251-256.
[16]LU X H,ZHU S S,BI Y,et al.Baseline sensitivity and resistance-risk assessment of Phytophthora capsici to iprovalicarb[J].Phytopathology,2010,100(11):1162-1168.
[17]ZHOU Y X,CHEN L,HU J,et al.Baseline sensitivity of natural population and resistance risk of Peronophythora litchii to four novel QoI fungicides[J].Eur J Plant Pathol,2016,146(1):71-83.
[18]邓维萍,杨敏,杜飞,等.葡萄胶孢炭疽菌对3种麦角甾醇脱甲基抑制剂类杀菌剂的敏感性[J].农药学学报,2011,13(3):245-252.DENG W P,YANG M,DU F,et al.Sensitivity of Colletotrichum gloeosporioides causing grape anthracnose to three sterol demethylation inhibitor(DMI)fungicides[J].Chin J Pestic Sci,2011,13(3):245-252.