海南芒果蒂腐病对8种杀菌剂的抗药性测定
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摘要
[目的]明确海南芒果蒂腐病(Botryodiplodia theobromae)对8种杀菌剂的抗药性。[方法]以10 mg/L为鉴别剂量进行抗药性初步检测,采用菌丝生长抑制法测定EC50值。[结果]2个苯并咪唑类杀菌剂抗性频率分别为87.5%和90.1%;甾醇脱甲基抑制剂抗性频率在17.1%~74.9%之间。供试菌株对50%多菌灵WP的EC50值在3.23~33 113.11 mg/L之间;对70%甲基硫菌灵WP的EC50值在1.76~11 091.75 mg/L之间,50%多菌灵WP与70%甲基硫菌灵WP之间存在交互抗性。[结论]海南芒果可可球二胞对苯并咪唑类杀菌剂普遍存在抗药性。
[Aims] The study aims to clarify the resistance of Botryodiplodia the obromae to 8 fungicides. [Methods] The62 strains of B. theobromae were collected from mango orchard in Hainan to detected the resistance to 8 fungicides with10 mg/L as the distinctive concentration. The EC50 values were identified by the method of hypha growth inhibition of B. theobromae. [Results] The strains showed different level of resistance to all the eight fungicides, with a high frequency of 87.5 and 90.1% to benzimidazole fungicides, and the frequency of strobilurin fungicides ranged from17.1-74.9%. The EC50 values of 10 carbendazim-resistant strains ranged from 3.23-33 113.11 mg/L, the EC50 values of10 thiophanate-methyl-resistant strains ranged from 1.76-11 091.75 mg/L. The linear regression showed that there was cross-resistance between carbendazim 50% WP and thiophanate-methyl 70% WP, but there was no cross-resistance between difenoconazole 10% WG and prochlora 50% WP with low level of resistance. [Conclusions] The fungicide resistance of B. theobromae to benzimidazole was commonly present in Hainan.
[Aims] The study aims to clarify the resistance of Botryodiplodia the obromae to 8 fungicides. [Methods] The62 strains of B. theobromae were collected from mango orchard in Hainan to detected the resistance to 8 fungicides with10 mg/L as the distinctive concentration. The EC50 values were identified by the method of hypha growth inhibition of B. theobromae. [Results] The strains showed different level of resistance to all the eight fungicides, with a high frequency of 87.5 and 90.1% to benzimidazole fungicides, and the frequency of strobilurin fungicides ranged from17.1-74.9%. The EC50 values of 10 carbendazim-resistant strains ranged from 3.23-33 113.11 mg/L, the EC50 values of10 thiophanate-methyl-resistant strains ranged from 1.76-11 091.75 mg/L. The linear regression showed that there was cross-resistance between carbendazim 50% WP and thiophanate-methyl 70% WP, but there was no cross-resistance between difenoconazole 10% WG and prochlora 50% WP with low level of resistance. [Conclusions] The fungicide resistance of B. theobromae to benzimidazole was commonly present in Hainan.
引文
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[14]王丽,陈鹏,周益林,等.2009年我国部分麦区小麦白粉菌群体对三唑酮和嘧菌酯的敏感性测定[J].植物病理学报,2011,41(6):654-658.
[15]周明国,叶钟音,刘经芬.杀菌剂抗性研究进展[J].南京农业大学学报,1994,17(3):33-41.
[16]SWART S H,SERFONTEIN J J,KALINNOWSKI J.Chemical Control of Post-harvest Diseases of Mango the Effect of Prochloraz,Thiobendazole and Fludioxonil on Soft Brown Rot,Stem-end Rot and Anthracnose[C].ISHS Acta Horticulturae 820:VIII International Mango Symposium,2009.
[17]LUO C X,COX K D,AMIRI A,et al.Occurrence and Detection of the DMI Resistance-associated Genetic Element‘Mona’in Monilinia fruc tic ola[J].Plant Disease,2008,92(7):1099-1103.
[2]胡美姣,高兆银,李敏,等.芒果果实潜伏侵染真菌种类研究[J].果树学报,2012,29(1):105-110.
[3]HONG S K,LEE S Y,CHIO H W,et al.Occurrence of Stem-end Rot on Mango Fruits Caused by Lasiodiplodia theobromae in Korea[J].The Plant Pathology Journal,2012,28(4):455.
[4]胡美姣,杨冬平,杨波,等.芒果树回枯病病原菌鉴定及其生物学特性研究[J].热带作物学报,2012(1):122-126.
[5]SAKALIDIS M L,RAY J D,LANOISELET V,et al.Pathogenic Botryos p haeriac eae Associated with Mangifera indica in the Kimberley Region of Western Australia[J].European Journal of Plant Pathology,2011,130(3):379-391.
[6]RBEZ-TORRES J R,LEAVITT G M,GUERRERO J C,et al.Identification and Pathogenicity of Lasiodiplodia theobromae and Dip lodia s eriata,the Causal Agents of Bot Canker Disease of Grapevines in Mexico[J].Plant Disease,2008,92(4):519-529.
[7]杨叶,何书海,张淑娟,等.海南芒果炭疽菌对多菌灵的抗药性测定[J].热带作物学报,2008,29(1):73-77.
[8]KUMAR A S,REDDY N P E,REDDY K H,et al.Evaluation of Fungicidal Resistance among Colletotrichum gloeosporioides Isolates Causing Mango Anthracnose in Agri Export Zone of Andhra Pradesh India[J].Plant Pathology Bulletin,2007,16:157-160.
[9]KONGTRAGOUL P,NALUMPANG S,MIYAMOTO Y,et al.Mutation at Codon 198 of Tub2 Gene for Carbendazim Resistance in Colletotrichum gloeosporioides Causing Mango Anthracnose in Thailand[J].Journal of Plant Protection Research,2011,51(4):377-384.
[10]胡美姣,师超,安勇,等.芒果蒂腐病菌对多菌灵的抗药性测定及其杀菌剂筛选[J].果树学报,2009,26(5):671-677.
[11]匡静,王建新,周明国.江苏省油菜菌核病菌对多菌灵和菌核净的抗药性监测[J].中国农学通报,2011,27(15):285-291.
[12]BYUNGSUP K,EUNWOO P,YUN C K.Population Dynamics of Sensitive and Resistance Phenotypes of Botrytis cinerea to Benzimidazole,Dicarboximide and N-Phenylcarbamate Fungicides in Korea[J].Journal of Pesticide Science,2000,25(4):385-386.
[13]乔广行,严红,么奕清,等.北京地区番茄灰霉病菌的多重抗药性检测[J].植物保护,2011,37(5):176-180.
[14]王丽,陈鹏,周益林,等.2009年我国部分麦区小麦白粉菌群体对三唑酮和嘧菌酯的敏感性测定[J].植物病理学报,2011,41(6):654-658.
[15]周明国,叶钟音,刘经芬.杀菌剂抗性研究进展[J].南京农业大学学报,1994,17(3):33-41.
[16]SWART S H,SERFONTEIN J J,KALINNOWSKI J.Chemical Control of Post-harvest Diseases of Mango the Effect of Prochloraz,Thiobendazole and Fludioxonil on Soft Brown Rot,Stem-end Rot and Anthracnose[C].ISHS Acta Horticulturae 820:VIII International Mango Symposium,2009.
[17]LUO C X,COX K D,AMIRI A,et al.Occurrence and Detection of the DMI Resistance-associated Genetic Element‘Mona’in Monilinia fruc tic ola[J].Plant Disease,2008,92(7):1099-1103.