北京地区莴苣中番茄斑萎病毒的鉴定
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摘要
番茄斑萎病毒(Tomato spotted wilt virus,TSWV)是目前已知的寄主范围最广、危害最严重的植物病毒之一。采集北京市顺义地区疑似感染病毒病的莴苣叶片,症状表现为严重的叶片斑驳褪绿、矮化,甚至整株枯萎死亡,发病率可达25%以上;结合发病症状和分子检测技术,证明其病原为番茄斑萎病毒属的TSWV。进一步基于TSWV外壳蛋白基因序列对不同国家和国内其他地区TSWV的进化关系进行了比较分析,结果显示北京地区莴苣中的TSWV与国内已报道的TSWV差异较大,而与西班牙分离的TSWV进化关系最近,表明该TSWV病毒非国内其他地区传播,而是由国外直接输入的病例。
Tomato spotted wilt virus(TSWV),with the most wide host range,is also one of the most devastating plant viruses.A serious lettuce virus disease was observed in a greenhouse in Shunyi District,Beijing. The infected symptoms exhibited as chlorotic spots in leaves,internode shortening,black streaks on stems,and even dieback in whole plants.According to preliminary statistics,the disease incidence is over 25%.These symptoms are highly similar to those on lettuce infected with TSWV.To identify the pathogen,the infected lettuce were identified by RT-PCR method with TSWV-specific primers.The results approved that all tested samples were infected with TSWV.Base on NP gene,phylogenetic tree between TSWV isolated in this study and other TSWV reported in other regions and foreign countries were constructed.The tree indicated that the new TSWV identify in this study is distinctly different from TSWV reported in China,but close cluster with TSWV isolated in Spain and other foreign countries,indicating that the new TSWV in Beijing may be a new invasive species invaded and accompanied with plants introduction.
Tomato spotted wilt virus(TSWV),with the most wide host range,is also one of the most devastating plant viruses.A serious lettuce virus disease was observed in a greenhouse in Shunyi District,Beijing. The infected symptoms exhibited as chlorotic spots in leaves,internode shortening,black streaks on stems,and even dieback in whole plants.According to preliminary statistics,the disease incidence is over 25%.These symptoms are highly similar to those on lettuce infected with TSWV.To identify the pathogen,the infected lettuce were identified by RT-PCR method with TSWV-specific primers.The results approved that all tested samples were infected with TSWV.Base on NP gene,phylogenetic tree between TSWV isolated in this study and other TSWV reported in other regions and foreign countries were constructed.The tree indicated that the new TSWV identify in this study is distinctly different from TSWV reported in China,but close cluster with TSWV isolated in Spain and other foreign countries,indicating that the new TSWV in Beijing may be a new invasive species invaded and accompanied with plants introduction.
引文
车海彦,曹学仁,刘培培,罗大全.2017.海南省冬季蔬菜病毒病发生情况调查.热带农业科学,37(1):71-74.
高苇,王勇,张春祥,张安盛,竺晓平.2016.天津地区辣椒病毒病调查及毒源种类初步鉴定.山东农业科学,48(3):91-94.
李飞,吴青君,徐宝云,谢文,王少丽,张友军.2012.北京地区发现番茄斑萎病毒.植物保护,38(6),186-188.
李洁,迟胜起,杨勤民,丁天波,褚栋.2017.山东烟台地区发生番茄斑萎病毒病危害.植物保护,43(1):228-232.
邱树亮,王孝宣,杜永臣,高建昌,国艳梅,朱德蔚,胡鸿,李宝聚,石延霞.2012.番茄斑萎病毒TSWV的鉴定及抗病种质的筛选.园艺学报,39(6):1107-1114.
苏大昆,袁宣泽,谢永红,王淑荣,丁海.1987.成都、渡口两市番茄中检出番茄斑萎病毒.植物病理学报,17(4):255-256.
许泽永,Reddy D V R,Rajeshwari R,张宗义,黄立新.1986.我国南方花生发生一种由番茄斑萎病毒引起的新病害.病毒学报,2(3):80-83.
许泽永,张宗义,陈金香.1989.番茄斑萎病毒(TSWV)广州分离物生物学特性研究.植物病理学报,19(4):198.
姚革.1992.四川晒烟上发现番茄斑萎病毒(TSWV).烟草科技,(6):2-4.
张友军,吴青君,徐宝云,朱国仁.2003.危险性外来入侵生物--西花蓟马在北京发生危害.植物保护,29(4):58-59.
张友军,朱国仁,褚栋,吴青君,王少丽.2011.我国蔬菜作物重大入侵害虫发生、危害与控制.植物保护,37(6):1-6.
张仲凯,杨录明,方琦,彭潞波,汪继玲,普耀芳.2004.番茄斑萎病毒侵染烟草的细胞病理.电子显微学报,23(4):349.
郑宽瑜,吴阔,董家红,方琦,张仲凯.2015.番茄斑萎病毒对云南莴苣类蔬菜的侵染危害.植物保护,41(5):174-178.
Chung B N,Pak H S,Jung J A,Kim J S.2006.Occurrence of Tomato spotted wilt virus in Chrysanthemum(Dendranthema grandiflorum)in Korea.Plant Pathology Journal,22(3):230-234.
Ciuffo M,Mammella M,Vallino M,Caciagli P,Turina M.2016.Molecular identification and biological characterization of a new potyvirus in lettuce.Archives of Virology,161(9):2549-2664.
Moreno A,Fereres A.2012.Virus diseases in lettuce in the Mediterranean basin.Advances in Virus Research,84:247-288.
Pappu H R,Culbreath A K,Bertrand P F,Csinos A S,Niblett CL.1996.Sequence analysis of the nucleocapsid protein gene of a Tomato spotted wilt virus isolate from Georgia,USA.Acta Horticulturae,431:237-243.
Parrella G,Gognalons P,Gebre-SelassièK,Vovlas C,Marchoux G.2003.An update of the host range of Tomato spotted wilt virus.Journal of Plant Pathology,85(4):227-264.
Sherwood J L,Sandbom M R,Keyser G C,Myers L D.1989.Use of monoclonal antibodies in detection of Tomato spotted wilt virus.Phytopathol,79(1):61-64.
Wu X,Chen C,Xiao X,Deng M J.2016.Development of reverse transcription thermostable helicase-dependent DNA amplification for the detection of Tomato spotted wilt virus.Journal of Aoac International,99(6):1596-1599.
高苇,王勇,张春祥,张安盛,竺晓平.2016.天津地区辣椒病毒病调查及毒源种类初步鉴定.山东农业科学,48(3):91-94.
李飞,吴青君,徐宝云,谢文,王少丽,张友军.2012.北京地区发现番茄斑萎病毒.植物保护,38(6),186-188.
李洁,迟胜起,杨勤民,丁天波,褚栋.2017.山东烟台地区发生番茄斑萎病毒病危害.植物保护,43(1):228-232.
邱树亮,王孝宣,杜永臣,高建昌,国艳梅,朱德蔚,胡鸿,李宝聚,石延霞.2012.番茄斑萎病毒TSWV的鉴定及抗病种质的筛选.园艺学报,39(6):1107-1114.
苏大昆,袁宣泽,谢永红,王淑荣,丁海.1987.成都、渡口两市番茄中检出番茄斑萎病毒.植物病理学报,17(4):255-256.
许泽永,Reddy D V R,Rajeshwari R,张宗义,黄立新.1986.我国南方花生发生一种由番茄斑萎病毒引起的新病害.病毒学报,2(3):80-83.
许泽永,张宗义,陈金香.1989.番茄斑萎病毒(TSWV)广州分离物生物学特性研究.植物病理学报,19(4):198.
姚革.1992.四川晒烟上发现番茄斑萎病毒(TSWV).烟草科技,(6):2-4.
张友军,吴青君,徐宝云,朱国仁.2003.危险性外来入侵生物--西花蓟马在北京发生危害.植物保护,29(4):58-59.
张友军,朱国仁,褚栋,吴青君,王少丽.2011.我国蔬菜作物重大入侵害虫发生、危害与控制.植物保护,37(6):1-6.
张仲凯,杨录明,方琦,彭潞波,汪继玲,普耀芳.2004.番茄斑萎病毒侵染烟草的细胞病理.电子显微学报,23(4):349.
郑宽瑜,吴阔,董家红,方琦,张仲凯.2015.番茄斑萎病毒对云南莴苣类蔬菜的侵染危害.植物保护,41(5):174-178.
Chung B N,Pak H S,Jung J A,Kim J S.2006.Occurrence of Tomato spotted wilt virus in Chrysanthemum(Dendranthema grandiflorum)in Korea.Plant Pathology Journal,22(3):230-234.
Ciuffo M,Mammella M,Vallino M,Caciagli P,Turina M.2016.Molecular identification and biological characterization of a new potyvirus in lettuce.Archives of Virology,161(9):2549-2664.
Moreno A,Fereres A.2012.Virus diseases in lettuce in the Mediterranean basin.Advances in Virus Research,84:247-288.
Pappu H R,Culbreath A K,Bertrand P F,Csinos A S,Niblett CL.1996.Sequence analysis of the nucleocapsid protein gene of a Tomato spotted wilt virus isolate from Georgia,USA.Acta Horticulturae,431:237-243.
Parrella G,Gognalons P,Gebre-SelassièK,Vovlas C,Marchoux G.2003.An update of the host range of Tomato spotted wilt virus.Journal of Plant Pathology,85(4):227-264.
Sherwood J L,Sandbom M R,Keyser G C,Myers L D.1989.Use of monoclonal antibodies in detection of Tomato spotted wilt virus.Phytopathol,79(1):61-64.
Wu X,Chen C,Xiao X,Deng M J.2016.Development of reverse transcription thermostable helicase-dependent DNA amplification for the detection of Tomato spotted wilt virus.Journal of Aoac International,99(6):1596-1599.