摘要
1日龄番鸭感染番鸭呼肠孤病毒(MDRV)后,应用LPAI方法检测MPV-GPV二联弱毒疫苗免疫后抗体变化规律,应用MTT法测定番鸭血液、脾脏和胸腺的B淋巴细胞增殖功能,探讨MDRV对B淋巴细胞功能的影响。结果显示番鸭感染MDRV后免疫MPV-GPV二联疫苗,IM 7 d时番鸭GPV-LPAI效价平均值1.5log2;随后缓慢上升,到IM 21-28 d时GPVLPAI效价平均值维持在约3log2。与单独免疫组相比,GPV-LPAI效价大大降低,不能提供有效保护。1日龄番鸭人工感染MDRV后,PI 7 d外周血T淋巴细胞增殖指数与对照组相比明显下降(P<0.05)。PI 14-28 d与对照组相比下降,差异极显著(P<0.01),表明MDRV感染抑制了B淋巴细胞增殖功能。结果表明,MDRV感染能抑制抗体应答和番鸭外周血和免疫器官中B细胞的增殖功能作用,造成B细胞功能抑制。
After 1-day-old muscovy ducks were infected with muscovy duck reovirus(MDRV), the change of antibody after immunization with MPV-GPV bivalent attenuated vaccine was detected by LPAI method. The results showed that muscovy ducks were immunized with MPV-GPV vaccine after MDRV infection.The proliferation of B lymphocytes in blood, spleen and thymus of muscovy ducks was determined by MTT method. the average GPV-LPAI antiboy titer of muscovy ducks was 1.5 log2 at IM 7 d. Then slowly rise, and that average titer was maintain at about 3 log2 at IM 21-28 d. GPV-LPAI antiboy titer was significantly lower than that of the immunized group alone, which did not provide effective protection. After 1-day-old muscovy ducks were artificially infected with MDRV,the proliferation index of peripheral blood B lymphocyteswas significantly lower than that in control group P< 0.05 in PI 7 d and P<0.01 in PI 14-28 days, showing that MDRV infection inhibited the proliferation of B lymphocytes. These results suggest that MDRV infection can inhibit antibody response and B cell proliferation, resulting in B cell function inhibition.
引文
[1]Kuntz-Simon G,Basdlklanchard P,Cherbonnel M,et al.Bachulovirus-expressed muscovy duck reovirusσCprotein induces serum neutralizing antibodies and protection against challenge[J].Vaccine,2002,20:3113-3122.
[2]胡奇林,陈少莺.番鸭呼肠孤病毒病研究进展[J].福建畜牧兽医,2004,26(增刊):7-9.
[3]Kuntz-Simon G,Le Gall-Recule G,de Boisseson C,et al.Muscovy duck reovirus sigma C protein is atypically encoded by the smallest genome segment[J].J Gen Virol,2002,83(5):1189-1200.
[4]胡奇林,陈少莺,江斌,等.一种新的番鸭疫病(暂名番鸭肝白点病)病原的发现[J].福建畜牧兽医,2000,22(6):1-3.
[5]Heffels-redmann U,Muller H,Kaleta E F.Structural and biological characteristics of reoviruses isolated from Muscovy duck(Carina moschata)[J].Avian Pathology,1992,21:481-491.
[6]郑腾,曹治云,张志灯.我国番鸭呼肠孤病毒病的研究现状[J].畜牧与兽医,2005,37(8):52-54.
[7]胡奇林,陈少莺,林天龙,等.应用PCR快速鉴别番鸭和鹅细小病毒[J].中国预防兽医学报,2001,23(6):447-450.
[8]程晓霞,陈少莺,朱小丽,等.番鸭小鹅瘟病毒的分离与鉴定[J].福建农业学报,2008,23(4):355-358.
[9]陈少莺,胡奇林,程晓霞,等.番鸭细小病毒和鹅细小病毒二联弱毒细胞苗的研究[J].兽医大学学报,2003,23(3):226-228.
[10]马彪,逄越,王旭,等.Pax5对B细胞分化发育调控作用的研究进展[J].免疫学杂志,2012,28(7):624-627.
[11]林锋强,朱小丽,陈少莺,等.MDRV和H9 AIV共感染对番鸭免疫反应的抑制作用[J].中国兽医学报,2011,31(8):1111-1113,1122.
[12]林锋强,陈少莺,蔡羲,等.番鸭呼肠孤病毒和H9亚型禽流感病毒共感染对番鸭脾脏免疫抑制作用[J].中国兽医学报,2012,32(7):975-979.
[13]陈少莺,胡奇林,江斌,等.番鸭呼肠孤病毒超微结构研究[J].中国兽医学报,2006(6):662-664.
[14]林锋强,陈少莺,朱小丽,等.番鸭呼肠孤病毒和H9亚型禽流感病毒共感染对番鸭法氏囊和抗体产生的影响[J].中国预防兽医学报,2012,34(2):133-137.
[15]王全溪,赵丽娟,苏凤,等.番鸭呼肠孤病毒B3株感染对雏番鸭细胞免疫功能的影响[J].福建农林大学学报,2009,38(4):380-383.
[16]陈志胜,马春全,卢玉葵,等.番鸭呼肠孤病毒诱导雏番鸭免疫器官细胞凋亡的研究[J].中国预防兽医学报,2005,27(6):457-458.
[17]卢玉葵,朱燕秋,马春全,等.番鸭感染呼肠弧病毒后免发器官组织浆细胞数量的变化[J].黑龙江畜牧兽医,2005(10):47-49.