甘薯毁灭性病毒病害(SPVD)的研究进展
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摘要
甘薯病毒病害(Sweetpotato virus diseases,SPVD)是由甘薯羽状斑驳病毒(Sweetpotato feathery mottle virus, SPFMV)和甘薯褪绿矮化病毒(Sweetpotato chlorotic stunt virus, SPCSV)双重感染和协同互作引起的甘薯毁灭性病毒病。该病害自2012年在中国发现以来扩展迅速,2015年给广东省湛江市甘薯生产造成了巨大的经济损失。为了加强甘薯病毒病的防控,降低其带来的损失,本研究归纳了SPVD分布范围及危害,分别总结了SPFMV和SPCSV的基因结构与功能、传播方式、主要的检测手段,认为发病严重地区可以从探明病毒株系种类切入、从SPCSV与SPFMV协生作用的分子机理深入,实现及时检测、及早预防和甘薯无毒化栽培。
Sweetpotato virus disease(SPVD), which is caused by the dual infection and synergistic interaction of Sweetpotato feathery mottle virus(SPFMV) and Sweetpotato chlorotic stunt virus(SPCSV), is a devastatingviral disease of sweetpotato. Since reported in China in 2012, it has been expended rapidly, and in 2015 itcaused huge economic losses to sweetpotato production in Zhanjiang of Guangdong. In order to strengthen theprevention and control of sweetpotato virus disease and reduce losses, the distribution and harm of SPVD aresummarized, and the gene structure and function, transmission and detecting means of SPFMV and SPCSV arereviewed respectively. It is suggested that in serious disease areas, detection of virus strains should be a keypoint, and molecular mechanism study about synergism between SPFMV and SPCSV should be furtherexplored, thus to achieve the timely detection, early prevention and non-toxic cultivation of sweetpotato.
Sweetpotato virus disease(SPVD), which is caused by the dual infection and synergistic interaction of Sweetpotato feathery mottle virus(SPFMV) and Sweetpotato chlorotic stunt virus(SPCSV), is a devastatingviral disease of sweetpotato. Since reported in China in 2012, it has been expended rapidly, and in 2015 itcaused huge economic losses to sweetpotato production in Zhanjiang of Guangdong. In order to strengthen theprevention and control of sweetpotato virus disease and reduce losses, the distribution and harm of SPVD aresummarized, and the gene structure and function, transmission and detecting means of SPFMV and SPCSV arereviewed respectively. It is suggested that in serious disease areas, detection of virus strains should be a keypoint, and molecular mechanism study about synergism between SPFMV and SPCSV should be furtherexplored, thus to achieve the timely detection, early prevention and non-toxic cultivation of sweetpotato.
引文
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[4]刘起丽.中国甘薯双生病毒种类鉴定、分子变异及检测方法研究[D].北京:中国农业大学,2015.
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[10]邢继英,杨永嘉.甘薯病毒病检测方法[J].植物保护,1995,21(2):38-40.
[11]Clark C A,Hoy M W.Effects of Common Viruses on Yield and Quality of Beauregard Sweetpotato in Louisiana[J].Plant Disease,2006,90(1):83-88.
[12]张振臣,乔奇,秦艳红,等.中国发现由甘薯褪绿矮化病毒和甘薯羽状斑驳病毒协生共侵染引起的甘薯病毒病害[J].植物病理学报,2012,42(3):328-333.
[13]Karyeija R F,Gibson R W,Jpt V.The significance of sweet potato feathery mottle virus in subsistence sweet potato production in Africa[J].Plant Disease,1998,82(1):4-15.
[14]Tairo F,Mukasa S B,Jones R A,et al.Unravelling the genetic diversity of the three main viruses involved in Sweet Potato Virus Disease(SPVD),and its practical implications[J].Molecular Plant Pathology,2005,6(2):199-211.
[15]Kreuze J F,Savenkov E I,Cuellar W,et al.Viral class 1 RNase IIIinvolved in suppression of RNA silencing[J].Journal of Virology,2005,79(11):7227.
[16]Untiveros M,Olspert A,Artola K,et al.A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing[J].Molecular Plant Pathology,2016,17(7):1111-1123.
[17]Schaefers G A,Terry E R.Insect transmission of sweet potato disease agents in Nigeria[J].Phytopathology,1976,66(5):642-645.
[18]Ames T,Smit N E J M,Braun A R,et al.Sweetpotato:Major pests,diseases,and nutritional disorders[M].Lima,Peru:International Potato Center(CIP),1997:66-70.
[19]Qiao Q,Zhang Z C,Qin Y H,et al.First report of Sweet potato chlorotic stunt virus infecting sweet potato in China[J].Plant Disease,2011,95(3):356-356.
[20]姜珊珊,谢礼,吴斌,等.山东甘薯主要病毒的鉴定及多样性分析[J].植物保护学报,2017,44(1):93-102.
[21]彭小琴,王浩然,张俊,等.湖北甘薯病毒病的检测与鉴定[J].中国植保导刊,2017,37(8):20-23.
[22]黄利利,Pham Binhdan,何芳练,等.广西甘薯病毒病的病原病毒种类检测[J].基因组学与应用生物学,2016,35(5):1213-1218.
[23]赵永强,张成玲,孙厚俊,等.甘薯病毒病复合体(SPVD)对甘薯产量的影响[J].西南农业学报,2012,25(3):909-911.
[24]Milgram M,Cohen J,Loebenstein G.Effects of sweet potato feathery mottle virus and sweet potato sunken vein virus on sweet potato yields and rates of reinfection of virus-free planting material in Israel[J].Phytoparasitica,1996,24(3):189-193.
[25]Njeru R W,Mburu M W K,Cheramgoi E,et al.Studies on the physiological effects of viruses on sweet potato yield in Kenya[J].Annals of Applied Biology,2015,145(1):71-76.
[26]周全卢,张玉娟,黄迎冬,等.甘薯病毒病复合体(SPVD)对甘薯产量形成的影响[J].Agricultural Science&Technology,2014,30(9):42-46.
[27]王爽,刘顺通,韩瑞华,等.不同时期嫁接感染甘薯病毒病(SPVD)对甘薯产量的影响[J].植物保护,2015,41(4):117-120.
[28]Doolittle S P,Harter L L.A graft-transmissible virus of sweet potato[J].Phytopathology,1945,35(9):695-704.
[29]李汝刚,蔡少华,Salazar L F.中国甘薯病毒的血清学检测[J].植物病理学报,1990,20(3):189-194.
[30]刘意,苏文瑾,雷剑,等.湖北省主栽甘薯品种病毒种类的血清学检测[J].湖北农业科学,2016,55(22):5821-5824.
[31]Steck M B.Interactions between a crinivirus,an ipomovirus and a potyvirus in coinfected sweetpotato plants[J].Plant Pathology,2006,55(3):458-467.
[32]Tairo F,Jones R A C,Valkonen J P T.Potyvirus Complexes in Sweetpotato:Occurrence in Australia,Serological and Molecular Resolution,and Analysis of the Sweet potato virus 2(SPV2)Component[J].Plant Disease,2006,90(9):1120-1128.
[33]Untiveros M,Quispe D,Kreuze J.Analysis of complete genomic sequences of isolates of the Sweet potato feathery mottle virus strains C and EA:molecular evidence for two distinct potyvirus species and two P1 protein domains[J].Archives of Virology,2010,155(12):2059-2063.
[34]Tugume A K,Cuéllar W J,Mukasa S B,et al.Molecular genetic analysis of virus isolates from wild and cultivated plants demonstrates that East Africa is a hotspot for the evolution and diversification of Sweet potato feathery mottle virus[J].Molecular Ecology,2010,19(15):3139-3156.
[35]Dolores L M,Yebron M G N,Jr,et al.Molecular and biological characterization of selected Sweet potato feathery mottle virus(SPFMV)strains in the Philippines[J].Crop Protection Newsletter,2012,37(2):29-37.
[36]王晓华,张振臣,乔奇,等.甘薯羽状斑驳病毒外壳蛋白基因的分子变异[J].植物保护,2012,38(2):114-116.
[37]Qin Y H,Zhang Z C,Qiao Q,et al.Molecular variability of sweet potato chlorotic stunt virus(SPCSV)and five potyviruses infecting sweet potato in China[J].Archives of Virology,2013,158(2):491-495.
[38]Sakai J,Mori M,Morishita T,et al.Complete nucleotide sequence and genome organization of sweet potato feathery mottle virus(Sstrain)genomic RNA:the large coding region of the P1 gene[J].Archives of Virology,1997,142:1553-1562.
[39]Chung B Y-W,Miller W A,Atkins J F,et al.An overlapping essential gene in the Potyviridae[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(15):5897-5902.
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