MAPK1基因多态性及mRNA表达与缺血性脑卒中神经功能缺损程度的相关分析
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摘要
目的探讨丝裂原活化蛋白激酶1(MAPK1)基因rs6928、rs13515、rs1063311多态性及MAPK1基因mRNA表达与缺血性脑卒中(IS)患者神经功能缺损严重程度的相关性。方法采用Massarray SNP基因分型技术对MAPK1基因的rs6928、rs13515、rs1063311多态性进行基因分型,运用qRT-PCR实验技术测定MAPK1基因mRNA表达水平,使用NIHSS量表对IS患者进行神经功能缺损评分。结果校正性别、年龄之后,在加性模型、隐性模型中,rs6928多态性与IS患者NIHSS评分具有相关性(P <0. 05)。MAPK1基因mRNA表达水平与IS患者NIHSS评分无相关性(P> 0. 05)。结论 MAPK1基因rs6928多态性可能会影响IS患者神经功能缺损的严重程度。
Objective To investigate the correlation between polymorphisms of mitogen-activated protein kinases 1( MAPK1) gene rs6928,rs13515 and rs1063311 and MAPK1 mRNA expression and the degree of neurological deficits in patients with ischemic stroke( IS). Methods Genotypes of MAPK1 gene rs6928,rs13515 and rs1063311 were detected using Massarray SNP genotyping technique,MAPK1 mRNA expression was assessed by qRT-PCR,and the degree of neurological deficits in patients with IS was evaluated using NIHSS score. Results After adjusting for gender and age,the polymorphisms of rs6928 was associated with NIHSS score in patients with IS in the additive model and recessive model( P < 0. 05). The MAPK1 mRNA expression was not correlated to NIHSS score in IS patients( P > 0. 05).Conclusion The polymorphism of MAPK1 gene rs6928 may affect the degree of neurological deficits in patients with IS.
Objective To investigate the correlation between polymorphisms of mitogen-activated protein kinases 1( MAPK1) gene rs6928,rs13515 and rs1063311 and MAPK1 mRNA expression and the degree of neurological deficits in patients with ischemic stroke( IS). Methods Genotypes of MAPK1 gene rs6928,rs13515 and rs1063311 were detected using Massarray SNP genotyping technique,MAPK1 mRNA expression was assessed by qRT-PCR,and the degree of neurological deficits in patients with IS was evaluated using NIHSS score. Results After adjusting for gender and age,the polymorphisms of rs6928 was associated with NIHSS score in patients with IS in the additive model and recessive model( P < 0. 05). The MAPK1 mRNA expression was not correlated to NIHSS score in IS patients( P > 0. 05).Conclusion The polymorphism of MAPK1 gene rs6928 may affect the degree of neurological deficits in patients with IS.
引文
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[2]Wityk RJ,Pessin MS,Kaplan RF,et al.Serial assessment of acute stroke using the NIH Stroke Scale[J].Stroke,1994,25(2):362-365.
[3]Fabbri C,Crisafulli C,Calati R,et al.Neuroplasticity and second messenger pathways in antidepressant efficacy:pharmacogenetic results from a prospective trial investigating treatment resistance[J].Eur Arch Psychiatry Clin Neurosci,2017,267(8):723-735.
[4]Irving EA,Barone FC,Reith AD,et al.Differential activation of MAPK/ERK and p38/SAPK in neurones and glia following focal cerebral ischaemia in the rat[J].Brain Res Mol Brain Res,2000,77(1):65-75.
[5]Park HJ,Kim SK,Yun DH,et al.Association of Toll-like receptor 2 polymorphisms with National Institute of Health Stroke Scale scores of ischemic stroke patients[J].J Mol Neurosci,2012,46(3):536-540.
[6]Zhao H,Cao Y,Chen H,et al.The association between OPG rs3102735 gene polymorphism,microembolic signal and stroke severity in acute ischemic stroke patients[J].Gene,2017,613:25-29.
[7]Maddahi A,Edvinsson L.Cerebral ischemia induces microvascular pro-inflammatory cytokine expression via the MEK/ERK pathway[J].J Neuroinflammation,2010,7:14.
[8]Maddahi A,Povlsen GK,Edvinsson L.Regulation of enhanced cerebrovascular expression of proinflammatory mediators in experimental subarachnoid hemorrhage via the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase pathway[J].J Neuroinflammation,2012,9:274.
[9]Maddahi A,Kruse LS,Chen QW,et al.The role of tumor necrosis factor-αand TNF-αreceptors in cerebral arteries following cerebral ischemia in rat[J].J Neuroinflammation,2011,8:107.
[10]Luo F,Shi J,Shi Q,et al.Mitogen-Activated Protein Kinases and Hypoxic/Ischemic Nephropathy[J].Cell Physiol Biochem,2016,39(3):1051-1067.
[11]Antypa N,Souery D,Tomasini M,et al.Clinical and genetic factors associated with suicide in mood disorder patients[J].Eur Arch Psychiatry Clin Neurosci,2016,266(2):181-193.
[12]Liu Z,He D,Zhang X,et al.Neuroprotective effect of early and short-time applying sophoridine in p MCAO rat brain:down-regulated TRAF6 and up-regulated pERK1/2 expression,ameliorated brain infaction and edema[J].Brain Res Bull,2012,88(4):379-384.
[13]Kim VN,Nam JW.Genomics of microRNA[J].Trends Genet,2006,22(3):165-173.
[14]L¨ovkvist H,J¨onsson AC,Luthman H,et al.Variations in apolipoprotein D and sigma non-opioid intracellular receptor 1 genes with relation to risk,severity and outcome of ischemic stroke[J].BMC Neurol,2014,14:191.