加味黑布膏对大鼠耳廓复合痤疮模型血清IL-8和TNF-α的影响
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摘要
目的观察加味黑布膏对大鼠耳廓复合痤疮模型血清IL-8和TNF-α含量的影响,探讨外用加味黑布膏治疗痤疮的作用机制。方法将60只Wistar大鼠随机分为6组,每组10只,取任意1组做空白组,另外5组造模成功后随机分为模型组、阳性对照组、加味黑布膏低、中、高剂量组。空白组、模型组不涂抹任何药物,阳性对照组涂抹0. 025%维A酸乳膏,加味黑布膏低、中、高剂量组涂抹不同剂量的加味黑布膏,1次/d,连续给药4周后,采用ELISA法检测各组大鼠血清IL-8和TNF-α的含量。结果各治疗组大鼠血清IL-8和TNF-α的含量均低于模型组(P <0. 05);低、中剂量组与阳性对照组比较差异无统计学意义(P> 0. 05),高剂量组与阳性对照组比较差异有统计学意义(P <0. 05)。结论加味黑布膏治疗痤疮的作用机制可能与抑制和减少IL-8和TNF-α的产生有关。
Objective To observe the effect of modified Heibu ointment on serum IL-8 and TNF-αlevels in rat model with compound acne on the auricle,and to explore the mechanism of external application of modified Heibu ointment in the treatment of acne.Methods Sixty Wistar rats were randomly divided into 6 groups,with 10 rats in each group.One group was selected randomly as a blank group,and the other 5 groups were randomly divided into a model group,a positive control group,and a low,medium and high dose modified Heibu ointment groups after successful modeling.The blank group and the model group were not applied with any drug,the positive control group was applied with 0.025%vitamin A acid cream,and the low,medium and high dose modified Heibu ointment groups were applied with different doses of modified Heibu ointment once per day.After continuous administration of 4 weeks,IL-8 and TNF-αlevels in the serum of each group was detected by ELISA.Results IL-8 and TNF-αlevels in serum of the rats in each treatment group was lower than that in the model group(P<0.05).There was no statistical difference between the low and medium dose groups(P>0.05),and the difference between the high dose group and the positive control group was statistically significant(P<0.05).Conclusion Mechanism of modified Heibu ointment in the treatment of acne may be related to the inhibition and reduction of IL-8 and TNF-αlevels.
Objective To observe the effect of modified Heibu ointment on serum IL-8 and TNF-αlevels in rat model with compound acne on the auricle,and to explore the mechanism of external application of modified Heibu ointment in the treatment of acne.Methods Sixty Wistar rats were randomly divided into 6 groups,with 10 rats in each group.One group was selected randomly as a blank group,and the other 5 groups were randomly divided into a model group,a positive control group,and a low,medium and high dose modified Heibu ointment groups after successful modeling.The blank group and the model group were not applied with any drug,the positive control group was applied with 0.025%vitamin A acid cream,and the low,medium and high dose modified Heibu ointment groups were applied with different doses of modified Heibu ointment once per day.After continuous administration of 4 weeks,IL-8 and TNF-αlevels in the serum of each group was detected by ELISA.Results IL-8 and TNF-αlevels in serum of the rats in each treatment group was lower than that in the model group(P<0.05).There was no statistical difference between the low and medium dose groups(P>0.05),and the difference between the high dose group and the positive control group was statistically significant(P<0.05).Conclusion Mechanism of modified Heibu ointment in the treatment of acne may be related to the inhibition and reduction of IL-8 and TNF-αlevels.
引文
[1]马虎,余先华,张朝霞.红蓝光联合维胺脂等治疗中重度痤疮115例临床观察[J].中国皮肤性病学杂志,2015,29(1):104-106.
[2]Roodsari MR,Akbari MR,Sarrafi-rad N,et al.The effect of isotretinoin treatment on plasma homocysteine levels in acne vulgaris[J].Clin Exp Dermatol,2010,35(6):624-626.
[3]刘文彬,梁庆,王辉,等.一种痤疮复合动物模型的建立[J].广东药学院学报,2014,30(2):198-201.
[4]林姗姗,金鑫,黄炜,等.复方颠倒凝胶对大鼠耳廓痤疮模型治疗研究[J].天津中医药大学学报,2017,36(5):392-395.
[5]蒋震,陈德宇,李杰,等.红蓝光对炎症性痤疮患者白介素8和白介素17的影响[J].中国皮肤性病学杂志,2016,30(2):142-144.
[6]Ma Y,Chen Q,Liu Y,et al.Effects of 5-aminolevulinic acid photodynamic therapy on TLRs in acne lesions and keratinocytes co-cultured with P.acnes[J].Photo diagnosis and Photo dynther,2016,15(2):172-181.
[7]李瑶,吕德官,陈临溪.IL-8及其受体药物与疾病的研究进展[J].中国药理学通报,2014,30(3):310-314.
[8]张琳,顾炜,朴美淑,等.痤疮合剂的疗效及对兔耳痤疮模型动物中血清中IL-8、TNF-α含量的影响[J].上海医药,2017,38(3):65-68.
[9]崔莉,朱金土,王雅飞.中医药联合IPL治疗痤疮127例[J].陕西中医药大学学报,2014,37(1):53-58.
[10]夏毓,张震,张春贞,等.中医辨证论治配合红蓝光治疗寻常性痤疮90例临床观察[J].中国皮肤性病学杂志,2014,28(11):1166-1167.
[11]骆伟雄.痤疮中医分型与性激素水平变化关系[J].中国皮肤性病学杂志,2011,25(1):59-61.
[12]伍筱铭,王思农,刘青,等.三黄凝胶联合红蓝光外治Ⅱ、Ⅲ级痤疮的疗效观察[J].中国皮肤性病学杂志,2018,32(1):119-122.
[13]秦晋之,闫智勇.蜈蚣的药理作用和临床应用研究进展[J].河北农业科学,2008,12(10):164-166.
[14]赵子佳,周桂荣,王玉,等.蝉蜕的化学成分及药理作用研究[J].吉林中医药,2017,37(5):491-493.
[15]赵飞燕.五倍子的药理作用研究[J].世界最新医学信息文摘,2016,16(68):134-134.
[16]王磊.黑布膏治疗瘢痕的临床疗效观察[J].黑龙江医药,2010,23(4):626-627.
[17]刘久利,何静岩,赵文斌,等.中药治疗寻常痤疮组方的文献分析[J].实用皮肤病学杂志,2013,6(1):39-41.
[18]钟赣生.中药学[M].9版.北京:中国中医药出版社,2012:400.
[19]梅莉红,干慧慧,曾义斌,等.中药熏蒸治疗轻、中度寻常痤疮疗效观察及血清细胞因子水平检测[J].中国皮肤性病学杂志,2011,25(9):716-718.
[2]Roodsari MR,Akbari MR,Sarrafi-rad N,et al.The effect of isotretinoin treatment on plasma homocysteine levels in acne vulgaris[J].Clin Exp Dermatol,2010,35(6):624-626.
[3]刘文彬,梁庆,王辉,等.一种痤疮复合动物模型的建立[J].广东药学院学报,2014,30(2):198-201.
[4]林姗姗,金鑫,黄炜,等.复方颠倒凝胶对大鼠耳廓痤疮模型治疗研究[J].天津中医药大学学报,2017,36(5):392-395.
[5]蒋震,陈德宇,李杰,等.红蓝光对炎症性痤疮患者白介素8和白介素17的影响[J].中国皮肤性病学杂志,2016,30(2):142-144.
[6]Ma Y,Chen Q,Liu Y,et al.Effects of 5-aminolevulinic acid photodynamic therapy on TLRs in acne lesions and keratinocytes co-cultured with P.acnes[J].Photo diagnosis and Photo dynther,2016,15(2):172-181.
[7]李瑶,吕德官,陈临溪.IL-8及其受体药物与疾病的研究进展[J].中国药理学通报,2014,30(3):310-314.
[8]张琳,顾炜,朴美淑,等.痤疮合剂的疗效及对兔耳痤疮模型动物中血清中IL-8、TNF-α含量的影响[J].上海医药,2017,38(3):65-68.
[9]崔莉,朱金土,王雅飞.中医药联合IPL治疗痤疮127例[J].陕西中医药大学学报,2014,37(1):53-58.
[10]夏毓,张震,张春贞,等.中医辨证论治配合红蓝光治疗寻常性痤疮90例临床观察[J].中国皮肤性病学杂志,2014,28(11):1166-1167.
[11]骆伟雄.痤疮中医分型与性激素水平变化关系[J].中国皮肤性病学杂志,2011,25(1):59-61.
[12]伍筱铭,王思农,刘青,等.三黄凝胶联合红蓝光外治Ⅱ、Ⅲ级痤疮的疗效观察[J].中国皮肤性病学杂志,2018,32(1):119-122.
[13]秦晋之,闫智勇.蜈蚣的药理作用和临床应用研究进展[J].河北农业科学,2008,12(10):164-166.
[14]赵子佳,周桂荣,王玉,等.蝉蜕的化学成分及药理作用研究[J].吉林中医药,2017,37(5):491-493.
[15]赵飞燕.五倍子的药理作用研究[J].世界最新医学信息文摘,2016,16(68):134-134.
[16]王磊.黑布膏治疗瘢痕的临床疗效观察[J].黑龙江医药,2010,23(4):626-627.
[17]刘久利,何静岩,赵文斌,等.中药治疗寻常痤疮组方的文献分析[J].实用皮肤病学杂志,2013,6(1):39-41.
[18]钟赣生.中药学[M].9版.北京:中国中医药出版社,2012:400.
[19]梅莉红,干慧慧,曾义斌,等.中药熏蒸治疗轻、中度寻常痤疮疗效观察及血清细胞因子水平检测[J].中国皮肤性病学杂志,2011,25(9):716-718.