人参炙甘草附子配伍对小鼠急性毒性的影响
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摘要
目的:探讨附子与人参和炙甘草配伍对小鼠急性毒性的影响,旨在从毒性角度考察人参附子配伍、炙甘草附子配伍、人参炙甘草附子配伍是否减毒,为临床安全合理用药提供依据。方法:分别制备附子水提、人参附子水提、炙甘草附子水提和人参炙甘草附子水提药物,采用经典的急性毒性实验方法测定其半数致死量LD_(50)。结果:附子水提组分LD_(50)为22.862 g/kg,人参附子水提组分LD_(50)为31.346 g/kg,炙甘草附子水提组分LD_(50)为29.804 g/kg,人参炙甘草附子水提组分LD_(50)为29.962 g/kg。通过观察小鼠自主活动,死亡情况、摄食量、体质量和心、肝、脾、肺、肾各脏器指数的变化,可知人参附子配伍、炙甘草附子配伍和人参炙甘草附子配伍具有一定的减毒作用。总结:人参、炙甘草对附子具有一定的减毒作用,其减毒强度为:人参附子配伍>人参炙甘草附子配伍>炙甘草附子配伍。
Objective: To explore the effects on mice acute toxicity of combination, to investigate the attenuate effect of different combination of ginseng, roasted liquorice and radix aconiti chamichaeli, which provides a basis for clinical safe and rational application. Methods:radix aconiti chamichaeli water extract, ginseng combined radix aconiti chamichaeli water extract,roasted liquorice combined radix aconiti chamichaeli water extract and ginseng combined radix aconiti chamichaeli, roasted liquorice water extract were separately prepared. The lethal dose LD_(50) was measured by acute toxicity method. Results: LD_(50) of radix aconiti chamichaeli water extract was 22.862 g/kg, LD_(50) of ginseng combined with radix aconiti chamichaeli water extract was 31.346 g/kg, LD_(50) of roasted liquorice combined with radix aconiti chamichaeli water extract was 29.804 g/kg,and LD_(50) of ginseng combined with radix aconiti chamichaeli, roasted liquorice water extract was 29.962 g/kg. The spontaneous activity and behavioral changes of mice were observed, the death, food intake and index of body weight, heart,liver, spleen, lung and kidney were recorded to study the attenuating effects of different combination. Conclusion: Ginseng and roasted liquorice have certain attenuating effects on radix aconiti chamichaeli. Their attenuating effects were shown in order: ginseng combined with radix aconiti chamichaeli > ginseng combined with roasted liquorice and radix aconiti chamichaeli > roasted liquorice combined with radix aconiti chamichaeli.
Objective: To explore the effects on mice acute toxicity of combination, to investigate the attenuate effect of different combination of ginseng, roasted liquorice and radix aconiti chamichaeli, which provides a basis for clinical safe and rational application. Methods:radix aconiti chamichaeli water extract, ginseng combined radix aconiti chamichaeli water extract,roasted liquorice combined radix aconiti chamichaeli water extract and ginseng combined radix aconiti chamichaeli, roasted liquorice water extract were separately prepared. The lethal dose LD_(50) was measured by acute toxicity method. Results: LD_(50) of radix aconiti chamichaeli water extract was 22.862 g/kg, LD_(50) of ginseng combined with radix aconiti chamichaeli water extract was 31.346 g/kg, LD_(50) of roasted liquorice combined with radix aconiti chamichaeli water extract was 29.804 g/kg,and LD_(50) of ginseng combined with radix aconiti chamichaeli, roasted liquorice water extract was 29.962 g/kg. The spontaneous activity and behavioral changes of mice were observed, the death, food intake and index of body weight, heart,liver, spleen, lung and kidney were recorded to study the attenuating effects of different combination. Conclusion: Ginseng and roasted liquorice have certain attenuating effects on radix aconiti chamichaeli. Their attenuating effects were shown in order: ginseng combined with radix aconiti chamichaeli > ginseng combined with roasted liquorice and radix aconiti chamichaeli > roasted liquorice combined with radix aconiti chamichaeli.
引文
[1]李晓骄阳,栾永福,孙蓉.附子不同组分对正常小鼠的急性毒性实验比较研究.中国药物警戒.2013,10(10)∶583～587.
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[9]周思思,马增春,梁乾德,等.人参附子不同比例配伍对小鼠急性毒性的影响.天津中医药,2013,30(01)∶43～46.
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[11]谢晓芳,李梦婷,代良萍,等.附子保健品刨附片及炮天雄的急性毒性研究.中国民族民间医药,2014,(20)∶18～19.
[12]高鹏,李建荣,叶祖光.附子黄芩配伍减毒存效研究.中华中医药杂志,2015,(7)∶2337～2340.
[13]孙世晓,许蔚,王凤,等.生附子、生半夏配伍后急性毒性的实验研究.中医药信息,2011,28(2)∶104～106.
[2]Liu M,Cao Y,Lv D,et al.Effect of processing on the alkaloids in Aconitum tubers by HPLC-TOF/MS.J Pharm Anal,2017,7(3)∶170～175.
[3]陈荣昌,孙桂波,张强,等.附子毒性研究进展.中国中药杂志,2013,38(8)∶1126～1129.
[4]肖勇,马增春,王宇光,等.参附注射液配伍对乌头碱诱发心律失常的减毒研究.中药药理与临床,2013,(3)∶12～15.
[5]李敏,王阶,何庆勇,等.《中华人民共和国药典》收载治疗失眠中成药的配伍规律.中医杂志,2016,57(7)∶558～562.
[6]孙付军,周倩,王春芳,等.甘草炮制前后药效学比较.中国实验方剂学杂志,2010,16(14)∶115～118.
[7]詹淑玉,邵青,李正,等.生脉注射液中人参皂苷Rg1、Rb1在心肌缺血大鼠体内的药动学-药效学结合研究.中国中药杂志,2014,39(7)∶1300～1305.
[8]Tian L,Shen D,Li X,et al.Ginsenoside Rg3 inhibits epithelial-mesenchymal transition (EMT) and invasion of lung cancer by down-regulating FUT4.Oncotarget,2016,7(2):1619～1632.
[9]周思思,马增春,梁乾德,等.人参附子不同比例配伍对小鼠急性毒性的影响.天津中医药,2013,30(01)∶43～46.
[10]程显怡,马骏,李强,等.附子与瓜蒌不同配比急性毒性比较.西部中医药,2013,26(3)∶13～15.
[11]谢晓芳,李梦婷,代良萍,等.附子保健品刨附片及炮天雄的急性毒性研究.中国民族民间医药,2014,(20)∶18～19.
[12]高鹏,李建荣,叶祖光.附子黄芩配伍减毒存效研究.中华中医药杂志,2015,(7)∶2337～2340.
[13]孙世晓,许蔚,王凤,等.生附子、生半夏配伍后急性毒性的实验研究.中医药信息,2011,28(2)∶104～106.