食管鳞癌组织中磷酸化酪氨酸激酶2的表达及意义
|
摘要
目的探讨磷酸化酪氨酸激酶2(p-JAK2)在食管鳞癌(ESCC)组织中的表达及意义。方法选取2015年1月至2018年1月期间收治的90例ESCC患者,均进行活体组织穿刺和病理切片,用免疫组化法检测ESCC组织与癌旁正常食管黏膜组织中p-JAK2蛋白表达,并分析p-JAK2蛋白表达与性别、年龄及分化程度等临床病理参数的关系。结果 ESCC组织中p-JAK2蛋白阳性表达率高于癌旁正常食管黏膜组织(P<0.05);p-JAK2蛋白在不同分化程度的ESCC组织中表达差异有统计学意义(P<0.05),高分化的ESCC组织中p-JAK2蛋白阳性表达率高于中、低分化的ESCC组织(P均<0.05),中分化的ESCC组织中p-JAK2蛋白表达率高于低分化的ESCC组织(P<0.05);性别、年龄、浸润程度、病理形态、淋巴结转移及TNM分期等临床病理参数与ESCC组织中p-JAK2蛋白的阳性表达率无关(P均>0.05)。p-JAK2蛋白表达与ESCC的分化程度呈正相关(r_s=0.512,P=0.010)。结论 ESCC组织中p-JAK2蛋白的表达与其分化程度有关,通过检测p-JAK2蛋白的表达可为ESCC患者的预后判断提供参考。
Objective To investigate the expression and significance of phosphorylated choline kinase 2(p-JAK2) in the esophageal squamous cell carcinoma(ESCC).Methods Ninety patients with ESCC admitted to our hospital from January 2015 to January 2018 were examined by biopsy and pathological section. The expressions of p-JAK2 protein in the ESCC tissues and the adjacent normal esophageal mucosa tissues were detected by immunohistochemistry. The correlation between the expression of p-JAK2 protein and clinicopathological parameters was analyzed too.Results The positive rate of p-JAK2 protein expression in the ESCC was higher than that in the adjacent normal esophageal mucosa tissues(P<0.05). The expression of p-JAK2 protein was significantly different in the different differentiation degrees(P<0.05), the p-JAK2 protein positive expression rate of the highly differentiated ESCC was higher than those in the moderately differentiated ESCC and the poorly differentiated ESCC(P<0.05), and the p-JAK2 protein positive expression rate of the moderately differentiated ESCC was higher than that in the poorly differentiated ESCC(P<0.05). There was no significant difference in the positive expression rate of p-JAK2 protein among the clinicopathological parameters such as morphology, lymph node metastasis and TNM stage(P>0.05). The expression of p-JAK2 protein was positively correlated with the differentiation degree of ESCC(r_s=0.512, P=0.010).Conclusion The expression of p-JAK2 protein is positively correlated with the differentiation degree of ESCC, detecting the expression of p-JAK2 protein can provide a reference for prognosis judgment of ESCC.
Objective To investigate the expression and significance of phosphorylated choline kinase 2(p-JAK2) in the esophageal squamous cell carcinoma(ESCC).Methods Ninety patients with ESCC admitted to our hospital from January 2015 to January 2018 were examined by biopsy and pathological section. The expressions of p-JAK2 protein in the ESCC tissues and the adjacent normal esophageal mucosa tissues were detected by immunohistochemistry. The correlation between the expression of p-JAK2 protein and clinicopathological parameters was analyzed too.Results The positive rate of p-JAK2 protein expression in the ESCC was higher than that in the adjacent normal esophageal mucosa tissues(P<0.05). The expression of p-JAK2 protein was significantly different in the different differentiation degrees(P<0.05), the p-JAK2 protein positive expression rate of the highly differentiated ESCC was higher than those in the moderately differentiated ESCC and the poorly differentiated ESCC(P<0.05), and the p-JAK2 protein positive expression rate of the moderately differentiated ESCC was higher than that in the poorly differentiated ESCC(P<0.05). There was no significant difference in the positive expression rate of p-JAK2 protein among the clinicopathological parameters such as morphology, lymph node metastasis and TNM stage(P>0.05). The expression of p-JAK2 protein was positively correlated with the differentiation degree of ESCC(r_s=0.512, P=0.010).Conclusion The expression of p-JAK2 protein is positively correlated with the differentiation degree of ESCC, detecting the expression of p-JAK2 protein can provide a reference for prognosis judgment of ESCC.
引文
[1]李陕区,兰淼,赵仁礼,等.蛋白酪氨酸磷酸酶受体D(PTPRD)在食管鳞状细胞癌组织中的表达及其临床意义[J].现代检验医学杂志,2016,43(1):1-4.
[2]周萌,刘保国,顾静,等. Raptor、Rictor和磷酸化Akt在日光性角化病、Bowen病及鳞状细胞癌中的表达[J].中国麻风皮肤病杂志,2017,33(3):132-136.
[3]中国抗癌协会食管癌专业委员会编.食管癌规范化诊疗指南[M].北京:中国协和医科大学出版社,2013:3-5.
[4]郭兰伟,石春雷,黄慧瑶,等.中国1996-2015年食管癌经济负担研究的系统综述[J].中华流行病学杂志,2017,38(1):102-109.
[5]田威,杨晶,戚旻,等.肝癌组织中NDRG2和AKT2及其磷酸化蛋白表达及意义[J].中华实用诊断与治疗杂志,2016,30(8):740-742.
[6]李玲玲,王升志,刘旭阳,等.富含脯氨酸的酪氨酸激酶2和磷酸化的丝氨酸/苏氨酸蛋白激酶在舌鳞状细胞癌组织中的表达及临床意义[J].华西口腔医学杂志,2018,36(1):29-32.
[7]谭豆豆,马蓉,刘涛,等. p-JAK2蛋白在食管鳞状细胞癌中的表达及意义[J].新疆医科大学学报,2018,41(3):281-283.
[2]周萌,刘保国,顾静,等. Raptor、Rictor和磷酸化Akt在日光性角化病、Bowen病及鳞状细胞癌中的表达[J].中国麻风皮肤病杂志,2017,33(3):132-136.
[3]中国抗癌协会食管癌专业委员会编.食管癌规范化诊疗指南[M].北京:中国协和医科大学出版社,2013:3-5.
[4]郭兰伟,石春雷,黄慧瑶,等.中国1996-2015年食管癌经济负担研究的系统综述[J].中华流行病学杂志,2017,38(1):102-109.
[5]田威,杨晶,戚旻,等.肝癌组织中NDRG2和AKT2及其磷酸化蛋白表达及意义[J].中华实用诊断与治疗杂志,2016,30(8):740-742.
[6]李玲玲,王升志,刘旭阳,等.富含脯氨酸的酪氨酸激酶2和磷酸化的丝氨酸/苏氨酸蛋白激酶在舌鳞状细胞癌组织中的表达及临床意义[J].华西口腔医学杂志,2018,36(1):29-32.
[7]谭豆豆,马蓉,刘涛,等. p-JAK2蛋白在食管鳞状细胞癌中的表达及意义[J].新疆医科大学学报,2018,41(3):281-283.