多囊卵巢综合征患者血浆骨膜蛋白水平变化及意义
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摘要
目的检测多囊卵巢综合征患者(PCOS)及正常人群血浆骨膜蛋白(periostin)水平变化,探讨其与胰岛素抵抗及肥胖的关系。方法收集初诊的PCOS患者68例(PCOS组),同时选择年龄及体质指数(BMI)匹配的正常育龄期女性72例(对照组),ELISA法测定两组的血浆periostin水平,比较periostin水平与相关生化指标水平,并探讨periostin与相关指标之间的关系,通过稳态模型(HOMA)评估胰岛素抵抗(HOMA-IR)。结果 PCOS组血浆periostin水平高于对照组[(32.5±5.5)vs(25.9±3.6)ng/mL,P<0.01],PCOS组及对照组超重或肥胖亚组血浆periostin高于正常体重亚组,相关性分析提示血浆periostin水平与BMI、腰臀比、空腹血糖、餐后2h血糖、空腹胰岛素、HOMA-IR、总胆固醇、低密度脂蛋白胆固醇呈正相关(P<0.01,P<0.05),以periostin水平为因变量行多元线性逐步回归分析提示BMI、HOMA-IR是血浆periostin水平的独立影响因素(P<0.01)。结论血浆periostin水平在新诊断的PCOS和超重或肥胖人群中升高,可能与胰岛素抵抗相关。
引文
[1] Diamanti-kandarakis E,Dunaif A.Insulin resistance and the polycystic ovary syndrome revisited:an update on mechanisms and implications[J].Endocr Rev,2012,33(6):981-1030.
[2] Rubin KH,Glintborg D,Nybo M,et al.Development and Risk Factors of Type 2 Diabetes in a Nationwide Population of Women With Polycystic Ovary Syndrome[J].J Clin Endocrinol Metab,2017,102(10):3848-3857.
[3] Teede HJ,Hutchison SK,Zoungas S.The management of insulin resistance in polycystic ovary syndrome[J].Trends Endocrinol Metab,2007,18(7):273-279.
[4] Lu Y,Liu X,Jiao Y,et al.Periostin promotes liver steatosis and hypertriglyceridemia through downregulation of PPARα[J].J Clin Invest,2014,124(8):3501-3513.
[5] Cohen JC,Horton JD,Hobbs HH.Human fatty liver disease:old questions,and new insights[J].Science,2011,332(6037):1519-1523.
[6] Nakazeki F,Nishiqa M,Horie T,et al.Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo[J].Sci Rep,2018,8(1):8553.
[7] Graja A,Garcia-Carrizo F,Jank AM,et al.Loss of periostin occurs in aging adipose tissue of mice and its genetic ablation impairs adipose tissue lipid metabolism[J].Aqing Cell,2018,17(5):e12810.
[8] Takeshita S,Kikuno R,Tezuka K,et al.Osteoblast-secific factor2:cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I[J].Biochem J,1993,294 (Pt 1):271-278.
[9] Conway SJ,Izuhara K,Kudo Y,et al.The role of periostin in tissue remodeling across health and disease[J].Cell Mol Life Sci,2017,71(7):1279-1288.
[10] Liu AY,Zheng H,Ouyang G.Periostin a multifunctional matricellular protein in inflammatory and tumor microenvironments[J].Matrix Biol,2014,37:150-156.
[11] Norris RA,Damon B,Mirouov V,et al.Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues[J].J Cell Biochem,2007,101(3):695-711.
[12] Yang Z,Zhang H,Niu Y,et al.Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects[J].Sci Rep,2016,25(6):37886.
[13] Polyzos SA,Kountouras J,Anastasilakis AD,et al.Circulating periostin in patients with nonalcoholic fatty liver disease[J].Endocrine,2017,56(2):438-441.
[14] Luo Y,Qu H,Wang H,et al.Plasma periostin levels are increased in chinese subjects with obesity and type 2 diabetes and are positively correlated with glucose and lipid parameters[J].Mediators Inflamm,2016,2016:6423637.
[15] Bolton,K Seqal,McMillan J,et al.Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus[J].Int J Obes(Lond),2009,33(10):1153-1165.
[16] Ali Khan R,Kapur P,Jain A,et al.Effect of orlistat on periostin,adiponectin,inflammatory markers and ultrasound grades of fatty liver in obese NAFLD patients[J].Ther Clin Risk Manag,2017,13:139-149.
[2] Rubin KH,Glintborg D,Nybo M,et al.Development and Risk Factors of Type 2 Diabetes in a Nationwide Population of Women With Polycystic Ovary Syndrome[J].J Clin Endocrinol Metab,2017,102(10):3848-3857.
[3] Teede HJ,Hutchison SK,Zoungas S.The management of insulin resistance in polycystic ovary syndrome[J].Trends Endocrinol Metab,2007,18(7):273-279.
[4] Lu Y,Liu X,Jiao Y,et al.Periostin promotes liver steatosis and hypertriglyceridemia through downregulation of PPARα[J].J Clin Invest,2014,124(8):3501-3513.
[5] Cohen JC,Horton JD,Hobbs HH.Human fatty liver disease:old questions,and new insights[J].Science,2011,332(6037):1519-1523.
[6] Nakazeki F,Nishiqa M,Horie T,et al.Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo[J].Sci Rep,2018,8(1):8553.
[7] Graja A,Garcia-Carrizo F,Jank AM,et al.Loss of periostin occurs in aging adipose tissue of mice and its genetic ablation impairs adipose tissue lipid metabolism[J].Aqing Cell,2018,17(5):e12810.
[8] Takeshita S,Kikuno R,Tezuka K,et al.Osteoblast-secific factor2:cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I[J].Biochem J,1993,294 (Pt 1):271-278.
[9] Conway SJ,Izuhara K,Kudo Y,et al.The role of periostin in tissue remodeling across health and disease[J].Cell Mol Life Sci,2017,71(7):1279-1288.
[10] Liu AY,Zheng H,Ouyang G.Periostin a multifunctional matricellular protein in inflammatory and tumor microenvironments[J].Matrix Biol,2014,37:150-156.
[11] Norris RA,Damon B,Mirouov V,et al.Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues[J].J Cell Biochem,2007,101(3):695-711.
[12] Yang Z,Zhang H,Niu Y,et al.Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects[J].Sci Rep,2016,25(6):37886.
[13] Polyzos SA,Kountouras J,Anastasilakis AD,et al.Circulating periostin in patients with nonalcoholic fatty liver disease[J].Endocrine,2017,56(2):438-441.
[14] Luo Y,Qu H,Wang H,et al.Plasma periostin levels are increased in chinese subjects with obesity and type 2 diabetes and are positively correlated with glucose and lipid parameters[J].Mediators Inflamm,2016,2016:6423637.
[15] Bolton,K Seqal,McMillan J,et al.Identification of secreted proteins associated with obesity and type 2 diabetes in Psammomys obesus[J].Int J Obes(Lond),2009,33(10):1153-1165.
[16] Ali Khan R,Kapur P,Jain A,et al.Effect of orlistat on periostin,adiponectin,inflammatory markers and ultrasound grades of fatty liver in obese NAFLD patients[J].Ther Clin Risk Manag,2017,13:139-149.