β-伴大豆球蛋白对仔猪抗营养阈值的研究进展
|
摘要
伴大豆球蛋白等抗原蛋白热稳定性较强,其抗原活性难以完全去除,可导致仔猪等幼龄动物产生过敏反应,从而影响其生理健康、日粮养分消化利用和生产性能。目前针对不同大豆制品在畜禽生产中的应用已有大量研究,但大豆抗原蛋白在幼龄畜禽日粮中的安全剂量尚不明确。本文对β-伴大豆球蛋白的理化性质及对仔猪的致敏机制、大豆制品中β-伴大豆球蛋白含量及大豆制品在仔猪生产中的应用进行综述,并对β-伴大豆球蛋白在仔猪日粮中的安全剂量进行探讨。
Soybean and its processed products are indispensably high-quality protein sources in piglet production. Antinutritional factors in soybean, especially β-conglycinin are generally difficult to be removed completely due to their strong thermal stability and antigenic activity. They may cause allergic reactions in young animals, such as piglets, thus affecting their physiological health, nutrient digestion and utilization, and production performance. Therefore, the anti-nutritional threshold of β-conglycinin for piglets is an important reference standard for determining the safe and efficient application of soybean and its products in piglet diets. The anti-nutritional threshold of β-conglycinin in young livestock and poultry such as piglets is still unclear. In this paper, the detection method and content of antigenic protein in soybean products and the application effect of soybean products in piglet diet were discussed. The safe dosage of soybean antigen β-conglycinin in piglet diets which provide an important basis for the safe and efficient utilization of soybean and its products in piglets was proposed.
Soybean and its processed products are indispensably high-quality protein sources in piglet production. Antinutritional factors in soybean, especially β-conglycinin are generally difficult to be removed completely due to their strong thermal stability and antigenic activity. They may cause allergic reactions in young animals, such as piglets, thus affecting their physiological health, nutrient digestion and utilization, and production performance. Therefore, the anti-nutritional threshold of β-conglycinin for piglets is an important reference standard for determining the safe and efficient application of soybean and its products in piglet diets. The anti-nutritional threshold of β-conglycinin in young livestock and poultry such as piglets is still unclear. In this paper, the detection method and content of antigenic protein in soybean products and the application effect of soybean products in piglet diet were discussed. The safe dosage of soybean antigen β-conglycinin in piglet diets which provide an important basis for the safe and efficient utilization of soybean and its products in piglets was proposed.
引文
[1]刘欣,冯杰.大豆抗原蛋白的研究进展[J].中国饲料,2004(20):14-15.
[2]Wu J J,Zhang Y,Dong J H,et al.Allergens and intestinal damage induced by soybean antigen proteins in weaned piglets[J].Ital J Anim Sci,2016,15(3):1-9.
[3]Zheng S,Qin G,Tian H,et al.Role of soybeanβ-conglycinin subunits as potential dietary allergens in piglets[J].Vet J,2014199(3):434-438.
[4]Thanh V H,Shibasaki K.Heterogeneity of betaconglycinin[J].Biochim Biophys Acta,1976,439(2):326-338.
[5]Taliercio E,Loveless T M,Turano M J,et al.Identification of epitopes of theβsubunit of soybeanβ-conglycinin that are antigenic in pigs,dogs,rabbits and fish[J].J Sci Food Agric,2014,94(11):2289-2294.
[6]Sun X,Shan X,Yan Z,et al.Prediction and characterization of the linear IgE epitopes for the major soybean allergenβ-conglycinin using immunoinformatics tools[J].Food Chem Toxicol,2013,56(Complete):254-260.
[7]Guo P,Piao X,Cao Y,et al.Recombinant Soybean Proteinβ-Conglycininα'-Subunit Expression and Induced Hypersensitivity Reaction in Rats[J].Int Arch Allergy Immunol,2008,145(2):102-110.
[8]Krishnan H B,Kim W S,Jang S,et al.All Three Subunits of Soybeanβ-conglycinin are potential food allergens[J].J Agric Food Chem,2009,57(3):938-943.
[9]Ladics G S.Workshop overview:approaches to the assessment of the allergenic potential of food from genetically modified crops[J].Toxicol Sci,2003,73(1):8-16.
[10]韩鹏飞,姜学,马曦.大豆抗原蛋白研究进展[J].中国畜牧杂志,2009,45(19):69-72.
[11]Sun P,Li D,Li Z,et al.Effects of glycinin on IgE-mediated increase of mast cell numbers and histamine release in the small intestine[J].J Nutr Biochem,2008,19(9):627-633.
[12]游金明,王自蕊,瞿明仁,等.大豆抗原蛋白的生物学特性及其对仔猪的过敏反应[J].饲料工业,2007(21):53-53.
[13]Guzmán P,Salda A B,Cámara L,et al.Influence of soybean protein source on growth performance and nutrient digestibility of piglets from 21 to 57days of age[J].Anim Feed Sci Tech,2016,222(1):75-86.
[14]赵元,秦贵信,孙泽威,等.Glycinin和β-Conglycinin对不同生长阶段猪生长性能和粗蛋白消化率的影响[J].中国畜牧杂志,2011,47(19):35-40.
[15]王希春,徐述亮,王勇,等.大豆蛋白抗原对断奶仔猪生长性能、小肠消化酶活性及组胺水平的影响[J].中国兽医学报,2012,32(4):619-623,636.
[16]Yi D,Hou Y,Mei H,et al.β-Conglycinin enhances autophagy in porcine enterocytes[J].Amino Acids,2017,49(1):203-207.
[17]Zhao Y,Qin G,Han R,et al.β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2[J].Int J Mol Sci,2014,15(2):1915-1926.
[18]Mujoo R,Trinh D T,Ng P K W.Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture[J].Food Chem,2003,82(2):265-273.
[19]Meyer R,Chardonnens F,Hübner P,et al.Polymerase chain reaction(PCR)in the quality and safety assurance of food:detection of soya in processed meat products[J].Z Lebensm Untersu Forsch,1996,203(4):339-344.
[20]Concepción G M,Heras J M,Marina M L.Simple and rapid characterization of soybean cultivars by perfusion reversedphase HPLC:application to the estimation of the 11S and 7Sglobulin contents[J].J Sep Sci,2007,30(4):475.
[21]Ishikawa G,Takada Y,Nakamura T.A PCR-based method to test for the presence or absence ofβ-conglycininα'-andα-subunits in soybean[J].Mol Breeding,2006,17(4):365-374.
[22]Hei W,Li Z,Ma X,et al.Determination of beta-conglycinin in soybean and soybean products using a sandwich enzyme-linked immunosorbent assay[J].Anal Chim Acta,2012,734(none):62-68.
[23]You J,Li D,Qiao S,et al.Development of a monoclonal antibodybased competitive ELISA for detection ofβ-conglycinin,an allergen from soybean[J].Food Chem,2008,106(1):352-360.
[24]杨玉娟,姚怡莎,秦玉昌,等.豆粕与发酵豆粕中主要抗营养因子调查分析[J].中国农业科学,2016,49(3):573-580.
[25]姚怡莎,谷旭,商方方,等.大豆和膨化大豆主要抗营养因子分析[J].中国农业科学,2016,49(11):2174-2182.
[26]周天骄,谯仕彦,马曦,等.大豆饲料产品中主要抗营养因子含量的检测与分析[J].动物营养学报,2015,27(1):221-229.
[27]彭楠,刘建峰,梁运祥,等.间接竞争ELISA检测大豆11S球蛋白和7S伴球蛋白[J].华中农业大学学报,2007,26(5):661-664.
[28]Song Y S,Pérez V G,Pettigrew J E,et al.Fermentation of soybean meal and its inclusion in diets for newly weaned pigs reduced diarrhea and measures of immunoreactivity in the plasma[J].Anim Feed Sci Tech,2010,159(1-2):41-49.
[29]章世元,张杰,全丽萍,等.新型发酵豆粕在断奶仔猪日粮中应用效果[J].西北农业学报,2009,18(2):33-37.
[30]刘欣,刘树全.微生物发酵豆粕对仔猪生长性能及免疫功能的影响[J].粮食与饲料工业,2007(4):39-40.
[31]刘海燕,秦贵信,于维,等.发酵豆粕对仔猪生长性能、血液生化和抗氧化指标的影响[J].中国饲料,2010(17):19-21.
[32]Feng J,Liu X,Xu Z R,et al.Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets[J].Digest Dis Sci,2007,52(8):1845-1850.
[33]Feng J,Liu X,Xu Z R,et al.The effect of Aspergillus oryzae fermented soybean meal on growth performance,digestibility of dietary components and activities of intestinal enzymes in weaned piglets[J].Anim Feed Sci Tech,2007,134(3):295-303.
[34]Jeong J S,Park J W,Lee S I,et al.Apparent ileal digestibility of nutrients and amino acids in soybean meal,fish meal,spraydried plasma protein and fermented soybean meal to weaned pigs[J].Anim Sci J,2016,87(5):697-702.
[35]穆晓峰,雷华.日粮中添加乳清粉和膨化豆粕对断奶仔猪生产性能影响[J].畜牧与兽医,2007,39(8):30-31.
[36]Qiao S,Li D,Jiang J,et al.Effects of moist extruded full-fat soybeans on gut morphology and mucosal cell turnover time of weanling pigs[J].Asian-Australs J Anim Sci,2003,16(1):63-69.
[37]廖珂,王自蕊,游金明,等.不同处理豆粕及嗜酸乳杆菌培养物对断奶仔猪生长性能和肠道形态结构的影响[J].动物营养学报,2018,30(5):1872-1879.
[38]Xie Z,Hu L,Li Y,et al.Changes of gut microbiota structure and morphology in weaned piglets treated with fresh fermented soybean meal[J].J Microbiol Biotechn,2017,33(12):213.
[39]Cheng S S,Li Y,Geng S J,et al.Effects of dietary fresh fermented soybean meal on growth performance,ammonia and particulate matter emissions,and nitrogen excretion in nursery piglets[J].JZhejiang Univ Sci B,2017,18(12):1083-1092.
[40]黄琼.五指山小型猪在体消化模型和食物致敏模型的建立及其机制研究[D].北京:中国疾病预防控制中心,2010.
[41]曹程鸣,李宝,张瑜,等.日粮中添加大豆抗原蛋白对断奶仔猪血清中IgG和IgE水平以及肠道ZO-1表达的影响[J].中国兽医科学,2017(3):389-396.
[42]马良友.大豆抗原蛋白对断奶仔猪血清中细胞因子的影响及肠道致敏作用[D].合肥:安徽农业大学,2014.
[2]Wu J J,Zhang Y,Dong J H,et al.Allergens and intestinal damage induced by soybean antigen proteins in weaned piglets[J].Ital J Anim Sci,2016,15(3):1-9.
[3]Zheng S,Qin G,Tian H,et al.Role of soybeanβ-conglycinin subunits as potential dietary allergens in piglets[J].Vet J,2014199(3):434-438.
[4]Thanh V H,Shibasaki K.Heterogeneity of betaconglycinin[J].Biochim Biophys Acta,1976,439(2):326-338.
[5]Taliercio E,Loveless T M,Turano M J,et al.Identification of epitopes of theβsubunit of soybeanβ-conglycinin that are antigenic in pigs,dogs,rabbits and fish[J].J Sci Food Agric,2014,94(11):2289-2294.
[6]Sun X,Shan X,Yan Z,et al.Prediction and characterization of the linear IgE epitopes for the major soybean allergenβ-conglycinin using immunoinformatics tools[J].Food Chem Toxicol,2013,56(Complete):254-260.
[7]Guo P,Piao X,Cao Y,et al.Recombinant Soybean Proteinβ-Conglycininα'-Subunit Expression and Induced Hypersensitivity Reaction in Rats[J].Int Arch Allergy Immunol,2008,145(2):102-110.
[8]Krishnan H B,Kim W S,Jang S,et al.All Three Subunits of Soybeanβ-conglycinin are potential food allergens[J].J Agric Food Chem,2009,57(3):938-943.
[9]Ladics G S.Workshop overview:approaches to the assessment of the allergenic potential of food from genetically modified crops[J].Toxicol Sci,2003,73(1):8-16.
[10]韩鹏飞,姜学,马曦.大豆抗原蛋白研究进展[J].中国畜牧杂志,2009,45(19):69-72.
[11]Sun P,Li D,Li Z,et al.Effects of glycinin on IgE-mediated increase of mast cell numbers and histamine release in the small intestine[J].J Nutr Biochem,2008,19(9):627-633.
[12]游金明,王自蕊,瞿明仁,等.大豆抗原蛋白的生物学特性及其对仔猪的过敏反应[J].饲料工业,2007(21):53-53.
[13]Guzmán P,Salda A B,Cámara L,et al.Influence of soybean protein source on growth performance and nutrient digestibility of piglets from 21 to 57days of age[J].Anim Feed Sci Tech,2016,222(1):75-86.
[14]赵元,秦贵信,孙泽威,等.Glycinin和β-Conglycinin对不同生长阶段猪生长性能和粗蛋白消化率的影响[J].中国畜牧杂志,2011,47(19):35-40.
[15]王希春,徐述亮,王勇,等.大豆蛋白抗原对断奶仔猪生长性能、小肠消化酶活性及组胺水平的影响[J].中国兽医学报,2012,32(4):619-623,636.
[16]Yi D,Hou Y,Mei H,et al.β-Conglycinin enhances autophagy in porcine enterocytes[J].Amino Acids,2017,49(1):203-207.
[17]Zhao Y,Qin G,Han R,et al.β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2[J].Int J Mol Sci,2014,15(2):1915-1926.
[18]Mujoo R,Trinh D T,Ng P K W.Characterization of storage proteins in different soybean varieties and their relationship to tofu yield and texture[J].Food Chem,2003,82(2):265-273.
[19]Meyer R,Chardonnens F,Hübner P,et al.Polymerase chain reaction(PCR)in the quality and safety assurance of food:detection of soya in processed meat products[J].Z Lebensm Untersu Forsch,1996,203(4):339-344.
[20]Concepción G M,Heras J M,Marina M L.Simple and rapid characterization of soybean cultivars by perfusion reversedphase HPLC:application to the estimation of the 11S and 7Sglobulin contents[J].J Sep Sci,2007,30(4):475.
[21]Ishikawa G,Takada Y,Nakamura T.A PCR-based method to test for the presence or absence ofβ-conglycininα'-andα-subunits in soybean[J].Mol Breeding,2006,17(4):365-374.
[22]Hei W,Li Z,Ma X,et al.Determination of beta-conglycinin in soybean and soybean products using a sandwich enzyme-linked immunosorbent assay[J].Anal Chim Acta,2012,734(none):62-68.
[23]You J,Li D,Qiao S,et al.Development of a monoclonal antibodybased competitive ELISA for detection ofβ-conglycinin,an allergen from soybean[J].Food Chem,2008,106(1):352-360.
[24]杨玉娟,姚怡莎,秦玉昌,等.豆粕与发酵豆粕中主要抗营养因子调查分析[J].中国农业科学,2016,49(3):573-580.
[25]姚怡莎,谷旭,商方方,等.大豆和膨化大豆主要抗营养因子分析[J].中国农业科学,2016,49(11):2174-2182.
[26]周天骄,谯仕彦,马曦,等.大豆饲料产品中主要抗营养因子含量的检测与分析[J].动物营养学报,2015,27(1):221-229.
[27]彭楠,刘建峰,梁运祥,等.间接竞争ELISA检测大豆11S球蛋白和7S伴球蛋白[J].华中农业大学学报,2007,26(5):661-664.
[28]Song Y S,Pérez V G,Pettigrew J E,et al.Fermentation of soybean meal and its inclusion in diets for newly weaned pigs reduced diarrhea and measures of immunoreactivity in the plasma[J].Anim Feed Sci Tech,2010,159(1-2):41-49.
[29]章世元,张杰,全丽萍,等.新型发酵豆粕在断奶仔猪日粮中应用效果[J].西北农业学报,2009,18(2):33-37.
[30]刘欣,刘树全.微生物发酵豆粕对仔猪生长性能及免疫功能的影响[J].粮食与饲料工业,2007(4):39-40.
[31]刘海燕,秦贵信,于维,等.发酵豆粕对仔猪生长性能、血液生化和抗氧化指标的影响[J].中国饲料,2010(17):19-21.
[32]Feng J,Liu X,Xu Z R,et al.Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets[J].Digest Dis Sci,2007,52(8):1845-1850.
[33]Feng J,Liu X,Xu Z R,et al.The effect of Aspergillus oryzae fermented soybean meal on growth performance,digestibility of dietary components and activities of intestinal enzymes in weaned piglets[J].Anim Feed Sci Tech,2007,134(3):295-303.
[34]Jeong J S,Park J W,Lee S I,et al.Apparent ileal digestibility of nutrients and amino acids in soybean meal,fish meal,spraydried plasma protein and fermented soybean meal to weaned pigs[J].Anim Sci J,2016,87(5):697-702.
[35]穆晓峰,雷华.日粮中添加乳清粉和膨化豆粕对断奶仔猪生产性能影响[J].畜牧与兽医,2007,39(8):30-31.
[36]Qiao S,Li D,Jiang J,et al.Effects of moist extruded full-fat soybeans on gut morphology and mucosal cell turnover time of weanling pigs[J].Asian-Australs J Anim Sci,2003,16(1):63-69.
[37]廖珂,王自蕊,游金明,等.不同处理豆粕及嗜酸乳杆菌培养物对断奶仔猪生长性能和肠道形态结构的影响[J].动物营养学报,2018,30(5):1872-1879.
[38]Xie Z,Hu L,Li Y,et al.Changes of gut microbiota structure and morphology in weaned piglets treated with fresh fermented soybean meal[J].J Microbiol Biotechn,2017,33(12):213.
[39]Cheng S S,Li Y,Geng S J,et al.Effects of dietary fresh fermented soybean meal on growth performance,ammonia and particulate matter emissions,and nitrogen excretion in nursery piglets[J].JZhejiang Univ Sci B,2017,18(12):1083-1092.
[40]黄琼.五指山小型猪在体消化模型和食物致敏模型的建立及其机制研究[D].北京:中国疾病预防控制中心,2010.
[41]曹程鸣,李宝,张瑜,等.日粮中添加大豆抗原蛋白对断奶仔猪血清中IgG和IgE水平以及肠道ZO-1表达的影响[J].中国兽医科学,2017(3):389-396.
[42]马良友.大豆抗原蛋白对断奶仔猪血清中细胞因子的影响及肠道致敏作用[D].合肥:安徽农业大学,2014.