抗性和非抗性哈萨克羊人工感染包虫虫卵早期小肠免疫指标的差异分析
摘要
包虫病又称棘球蚴病,是由细粒棘球绦虫引起的一种呈全球性的人畜共患病。本研究就包虫病抗性和非抗性哈萨克羊人工口服感染了细粒棘球绦虫虫卵后,对小肠组织中的免疫球蛋白和主要的细胞因子和趋化因子进行了研究。研究发现人工感染后6h、8h、10h屠宰绵羊,采集绵羊各段小肠组织并匀浆取上清液,ELISA试剂盒检测小肠组织中抗体(IgM、IgG、IgE)、细胞因子(IL-2、TNF-α、IFN-γ、IL-4、sIgA)和趋化因子(CCL8)的水平。结果表明,IgE(P<0.05)在空肠后段、IgM(P<0.01)在回肠、SIgA(P<0.05)在十二指肠和空肠以及IFN-γ(P<0.05)在十二指肠和空肠前段,抗性组显著高于非抗性组,IgG(P<0.05)在空肠前段、IL-2(P<0.05)在回肠抗性组显著低于非抗性组。而TNF-α、IL-4和CCL8在两组间没有显著差异。这说明携带MHC抗性基因型的绵羊在小肠组织表现出较强的包虫病抵抗力可能与其早期IFN-γ、SIgA和IgM分泌较高有关。
引文
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[2]李仁燕.中国美利奴羊和哈萨克羊MHC-DRB1外显子2多态性与包虫病抗性研究[D].石河子大学,2011,博士论文.
[3]Rigano R E,Profumo A,Teggi,et al.Production of IL-5and IL-6by peripheral blood mononuclear cells(PBMC)from patients with Echinococcus granulosus infection[J].Clin Exp Immunol,1996,105:456-459.
[4]何维.医学免疫学,第2版[M].人民卫生出版社.
[5]郑宏,徐志新,汪师贞,等.宿主感染细粒棘球蚴免疫反应的研究进展[J].中国病原生物学杂志,2002,15(1):55-58.
[6]Wenqiao H,Song J,Jishun T,et al.An Immediate Innate Immune Response Occurred In the Early Stage of E.granulosus Eggs Infection in Sheep:Evidence from Microarray Analysis.[J].Plos One,2015,10(8).
[7]Zhang W,Allen G R,Donald P M.Mechanisms of Immunity in Hydatid Development Disease:Implications for Vaccine[J].The journal of immunology,2008,181:6679-6685.
[8]Thompson R C A,A J Lymbery.Echinococcus and Hydatid Disease[M],CABI,1995:496.
[9]Pater C,V Müller,M Harraga,et al.Intestinal and systemic humoral immunological events in the susceptible Balb/C mouse strain after oral administration of Echinococcus multilocularis eggs[J].Parasite Immunol,1998,20:623-629.
[10]Jinguo W,Wicker L S,Pere S.IL-2and its high-affinity receptor:genetic control of immunoregulation and autoimmunity.[J].Seminars in Immunology,2009,21(6):363-371.
[11]Li R,Qiang P,Bin J.Antibody and Cytokine Responses to Hydatid in Experimentally Infected Kazakh Sheep with Hydatidosis Resistance Haplotype[J].Parasitology Research.2011,108(5):1131-1137.
[12]Sandborn W J.Strategies for targeting tumour necrosis factor in IBD[J].Best Practice&Research Clinical Gastroenterology,2003,17(1):105-117.
[13]Benjamin N,Farrell C P,Maura B,et al.Tumor necrosis factor blockade for treatment of inflammatory bowel disease:efficacy and safety.[J].Current Molecular Pharmacology,2010,3(3):145-152.
[14]Baz A,Ettlin G M,Dematteis S.Complexity and function of cytokine responses in experimental infection by Echinococcus granulosus[J].Immunobiology,2006,211(1-2):3-9.
[15]惠文巧.MHC不同基因型哈萨克羊人工感染细粒棘球绦虫虫卵后小肠、肝脏差异表达基因的筛选[D].石河子大学,2013.
[16]Godot V,S Harraga,I Beurton,Resistance/susceptibility to echinococcus multilocularis infection and cytokine profile in humans.II.Influence of the HLA B8,DR3,DQ2haplotype[J].Clin Exp Immunol,2000,121:491-498.
[17]Emery I,Liance M,Leclerc C.Secondary Echinococcus multilocularis infection in A/J mice:delayed metacestode development is associated with Th1cytokine production.[J].Parasite Immunology,1997,19(11):493-503.
[18]Zhang W,H You,J Li,Z Zhang,et al.Immunoglobulin profiles in a murine intermediate host model of resistance for Echinococcus granulosus infection[J].Parasite Immunol,2003,25:161-168.
[19]West G A,Matsuura T,Levine A D,et al.Interleukin 4in inflammatory bowel disease and mucosal immune reactivity[J].Gastroenterology,1996,110(6):1683-1695.
[20]马彦博,白东英,李引乾,等.动物肠道黏膜免疫研究新进展[J].Livestock and Poultry Industry,2007(10):79-81.
[21]罗洪林,黄维义.动物黏膜免疫及其细胞因子[J].吉林畜牧兽医,2004(5):19-21.
[22]Dragoserrano M E,Godínezvictoria M,Larapadilla E,et al.Moderate exercise enhances expression of SIgA in mouse ileum.[J].International Journal of Sports Medicine,2012,33(12):1020-1025.
[23]Zhang WB,H Wen,J Li,et al.Immunology and Immunodiagnosis of Cystic Echinococcosis:An Update[J].Clin Dev Immunol,2012,2012:1-10.
[24]Asano K,Takahashi N,Ushiki M,et al.Intestinal CD169+macrophages initiate mucosal inflammation by secreting CCL8that recruits inflammatory monocytes[J].Nature Communications,2015,6.