高强度聚焦超声对大鼠卵巢癌的治疗效果及对免疫功能的影响
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摘要
目的探讨高强度聚焦超声(HIFU)对大鼠卵巢癌的治疗效果及对免疫功能的影响。方法选取40只SD大鼠构建原位卵巢癌模型,随机分为对照组、HIFU组,每组20只。HIFU组行局部HIFU治疗,对照组给予假照射处理。观察两组大鼠的生存状态,检测肿瘤生长情况;HE染色观察肿瘤组织形态学变化;利用流式细胞仪检测血浆中CD4+、CD8+水平。结果 HIFU组大鼠经HIFU治疗7 d生活状态无变化或有好转,对照组大鼠逐渐衰竭。HIFU组大鼠的中位生存时间为(48.0±4.5)d,明显长于对照组的(40.0±3.6)d(P<0.05)。HIFU组大鼠瘤体平均重量为(1.37±0.92)g,显著低于对照组的(2.86±0.73)g(P<0.05)。HIFU组大鼠卵巢组织发生显著形态学变化,血浆中CD4+较对照组显著增高,CD8+较对照组显著降低,CD4+/CD8+较对照组提高,差异均有统计学意义(P<0.05)。结论HIFU具有抑制肿瘤生长的作用,可能与增强机体免疫功能有关。
Objective To investigate the treatment effect of high intensity focused ultrasound on ovarian cancer and its influence on immune function of rats. Methods A total of 40 SD rats were selected to construct ovarian cancer in situ models and were randomly divided into control group and HIFU group. HIFU group was treated with local HIFU, while the control group was treated with sham irradiation. The survival state of rats in the two groups were observed, the growth of the tumor were detected, the histomorphological changes of the tumor were observed by HE staining, and the level of CD4+and CD8+in the plasma were detected by flow cytometry. Results In HIFU group, the living conditions of the rats were not changed or improved treated by HIFU for 7 days, while the rats in the control group gradually failed.The median survival time of rats in HIFU group was(48.0±4.5) d, which was significantly longer than that in the control group([40.0±3.6] d)(P<0.05). The average weight of tumor in HIFU group was(1.37±0.92) g, which was significantly lower than that in control group([2.86±0.73] g)(P<0.05). There were significant morphological changes in the ovarian tissue of rats in HIFU group, and the CD4+in the plasma was significantly higher than that in the control group,while the CD8+was significantly lower than the control group, and the CD4+/CD8+was higher than the control group,and the differences were statistically significant(P<0.05). Conclusion HIFU may inhibit tumor growth, which may be related to enhancing immune function of the body.
Objective To investigate the treatment effect of high intensity focused ultrasound on ovarian cancer and its influence on immune function of rats. Methods A total of 40 SD rats were selected to construct ovarian cancer in situ models and were randomly divided into control group and HIFU group. HIFU group was treated with local HIFU, while the control group was treated with sham irradiation. The survival state of rats in the two groups were observed, the growth of the tumor were detected, the histomorphological changes of the tumor were observed by HE staining, and the level of CD4+and CD8+in the plasma were detected by flow cytometry. Results In HIFU group, the living conditions of the rats were not changed or improved treated by HIFU for 7 days, while the rats in the control group gradually failed.The median survival time of rats in HIFU group was(48.0±4.5) d, which was significantly longer than that in the control group([40.0±3.6] d)(P<0.05). The average weight of tumor in HIFU group was(1.37±0.92) g, which was significantly lower than that in control group([2.86±0.73] g)(P<0.05). There were significant morphological changes in the ovarian tissue of rats in HIFU group, and the CD4+in the plasma was significantly higher than that in the control group,while the CD8+was significantly lower than the control group, and the CD4+/CD8+was higher than the control group,and the differences were statistically significant(P<0.05). Conclusion HIFU may inhibit tumor growth, which may be related to enhancing immune function of the body.
引文
[1]李秀琴,王欣彦.上皮性卵巢癌精准治疗研究进展[J].中国实用妇科与产科杂志,2016,33(8):810-813.
[2]张爽爽,夏庆民,郑荣寿,等.中国2010年卵巢癌发病与死亡分析[J].中国肿瘤,2016,25(3):169-173.
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[5]Gelet A,Crouzet S,Rouviere O,et al.High-intensity focused ultrasound(HIFU)for prostate cancer[J].Cancer Control,2007,14(3):244-249.
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[8]肖菊花,刘小红,白书华,等.大鼠Walker-256移植性卵巢癌模型的建立[J].中国妇幼保健,2015,30(34):6122-6124.
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[12]唐家伟,严飞,李叶阔.高强度聚焦超声联合纳米颗粒在肿瘤治疗中的研究进展[J].中国医学影像学杂志,2016,24(5):388-391.
[13]Dubinsky TJ,Cuevas C,Dighe MK,et al.High-intensity focused ultrasound:current potential and oncologic applications[J].AJR Am J Roentgenol,2008,190(1):191-199.
[14]王兴玲,于明新.苦参碱联合胸腺五肽对卵巢癌腹腔热化疗大鼠免疫功能的影响[J].实用药物与临床,2017,20(1):4-8.
[15]周颦,苏立,傅敏,等.高强度聚焦超声制备肿瘤疫苗对小鼠抗肿瘤免疫增强作用的研究[J].中国超声医学杂志,2012,28(7):591-594.
[16]潘振芳,任岚,王仁香,等.高强度聚焦超声治疗子宫肌瘤方法探讨及疗效评价[J].中国当代医药,2009,16(25):71-72.
[17]曾林静,邹建中.高强度聚焦超声在肝癌治疗中的临床应用[J].现代医药卫生,2016,32(10):1490-1492.
[18]胡泊.高强度聚焦超声在子宫肌瘤消融中的临床效果及影响因素研究[J].中国当代医药,2017,24(2):70-72.
[19]Kennedy JE.High-intensity focused ultrasound in the treatment of solid tumours[J].Nat Rev Cancer,2005,5(4):321-327.
[20]王智彪,郎景和.高强度聚焦超声消融与子宫腺肌病[J].中华妇产科杂志,2016,51(9):708-709.
[2]张爽爽,夏庆民,郑荣寿,等.中国2010年卵巢癌发病与死亡分析[J].中国肿瘤,2016,25(3):169-173.
[3]张玉霞,王晶.复发性卵巢癌的治疗进展[J].现代肿瘤医学,2016,10(11):1837-1840.
[4]王素萍,郑平燕.肿瘤标志物联合检测对早期卵巢癌的诊断价值研究[J].中国妇幼保健,2016,31(21):4427-4429.
[5]Gelet A,Crouzet S,Rouviere O,et al.High-intensity focused ultrasound(HIFU)for prostate cancer[J].Cancer Control,2007,14(3):244-249.
[6]王维红.高强度聚焦超声在肿瘤治疗中的应用研究[J].中国医学装备,2016,13(8):133-135.
[7]弓瑜,王璐.高强度聚焦超声对子宫肌瘤患者免疫功能的影响[J].实用癌症杂志,2017,32(12):2067-2069.
[8]肖菊花,刘小红,白书华,等.大鼠Walker-256移植性卵巢癌模型的建立[J].中国妇幼保健,2015,30(34):6122-6124.
[9]石一复,李娟清.高强度聚焦超声在妇产科的应用和设想[J].中国计划生育和妇产科,2017,9(12):1-4.
[10]Colen RR,Sahnoune I,Weinberg JS.Neurosurgical applications of high-intensity focused ultrasound with magnetic resonance thermometry[J].Neurosurg Clin N Am,2017,28(4):559-567.
[11]吴菲,曹兵生,张宏.高强度聚焦超声技术临床应用进展[J].武警医学,2010,21(8):701-703.
[12]唐家伟,严飞,李叶阔.高强度聚焦超声联合纳米颗粒在肿瘤治疗中的研究进展[J].中国医学影像学杂志,2016,24(5):388-391.
[13]Dubinsky TJ,Cuevas C,Dighe MK,et al.High-intensity focused ultrasound:current potential and oncologic applications[J].AJR Am J Roentgenol,2008,190(1):191-199.
[14]王兴玲,于明新.苦参碱联合胸腺五肽对卵巢癌腹腔热化疗大鼠免疫功能的影响[J].实用药物与临床,2017,20(1):4-8.
[15]周颦,苏立,傅敏,等.高强度聚焦超声制备肿瘤疫苗对小鼠抗肿瘤免疫增强作用的研究[J].中国超声医学杂志,2012,28(7):591-594.
[16]潘振芳,任岚,王仁香,等.高强度聚焦超声治疗子宫肌瘤方法探讨及疗效评价[J].中国当代医药,2009,16(25):71-72.
[17]曾林静,邹建中.高强度聚焦超声在肝癌治疗中的临床应用[J].现代医药卫生,2016,32(10):1490-1492.
[18]胡泊.高强度聚焦超声在子宫肌瘤消融中的临床效果及影响因素研究[J].中国当代医药,2017,24(2):70-72.
[19]Kennedy JE.High-intensity focused ultrasound in the treatment of solid tumours[J].Nat Rev Cancer,2005,5(4):321-327.
[20]王智彪,郎景和.高强度聚焦超声消融与子宫腺肌病[J].中华妇产科杂志,2016,51(9):708-709.