球孢白僵菌粉剂保存条件的研究
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摘要
本试验研究以延长球孢白僵菌粉剂储存条件为目标,拟从温度和化学因子展开,对粉剂进行孢子萌发率、玉米螟幼虫毒力和Pr1蛋白酶活性三方面检测。结果表明球孢白僵菌粉剂最佳配方是5号:95%白僵菌高孢粉+2%麦芽糖+3%蛋白胨,最佳储存温度是-20℃>4℃>室温。麦芽糖和蛋白胨是球孢白僵菌生长的必要营养成分,对球孢白僵菌孢子具有保护作用。
This research is aim to prolong the storage time of powder formulation of Beauveria bassiana. The im-pact factors include the temperature and the reagent. The germination rate, the virulence to Ostrinia furnacalis. andthe activity of Pr1 of conidia in the Beauveria bassiana powder formulation were detected. The results showed thatthe best Beauveria bassiana powder formulation was number 5: the 95% higher concentration spores powder of Beauveria bassiana. plus 2% maltose and 3% peptone. The optimum storage temperature was-20℃. The maltose andpeptone is the important carbon and nitrogen source to Beauveria bassiana, which have the protective function to pre-serve the spore activity of Beauveria bassiana.
This research is aim to prolong the storage time of powder formulation of Beauveria bassiana. The im-pact factors include the temperature and the reagent. The germination rate, the virulence to Ostrinia furnacalis. andthe activity of Pr1 of conidia in the Beauveria bassiana powder formulation were detected. The results showed thatthe best Beauveria bassiana powder formulation was number 5: the 95% higher concentration spores powder of Beauveria bassiana. plus 2% maltose and 3% peptone. The optimum storage temperature was-20℃. The maltose andpeptone is the important carbon and nitrogen source to Beauveria bassiana, which have the protective function to pre-serve the spore activity of Beauveria bassiana.
引文
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[2]陶淑霞,李玉,刘家富,等.球孢白僵菌对亚洲玉米螟幼虫血细胞数量和孢囊作用的影响[J].植物保护学报,2011,38(6):527-531.
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[4]汤坚,黄长春,丁珊,等.球孢白僵菌制剂载体的筛选[J].安徽农业大学学报,1996(3):351-354.
[5]田甜.飞蝗优良绿僵菌、白僵菌菌株的筛选及应用性研究[D].保定:河北农业大学,2009.
[6]刘健,陈洪章,李佐虎.白僵菌杀虫剂生产工艺研究状况与展望[J].中国生物防治,2003,19(2):86-90.
[7]朱昌雄,丁振华,蒋细良,等.微生物农药剂型研究发展趋势[J].现代化工,2003,23(3):4-8.
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[9]Feng M G,T J Poprawski.Robustness of the time-dose-mortality model in bioassay data analysis of microbial control agents and chemical agents for insect control[J].Subtropical Plant Science,1999(51):36-38.
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[11]彭国雄,张永军,杨星勇,等.球孢白僵菌不同世代菌株胞外蛋白酶与毒力的关系[J].中国生物防治,2000,16(2):61-64.
[12]程国华,舒静,丁克坚.球孢白僵菌营养需求及培养条件研究[J].中国农学通报,2006(5):365-368.
[13]张航,崔阳,董晓宇,等.球孢白僵菌孢子的耐逆性研究[J].哈尔滨师范大学自然科学学报,2014,30(5):104-107.
[14]应盛华,冯明光.真空干燥球孢白僵菌纯孢粉的活孢率、毒力与贮存期[J].微生物学通报,2002,29(5):42-47.
[15]王义生,朱晓敏,张荣宝,等.5种除草剂对白僵菌孢子萌发的影响[J].吉林农业科学,2015,40(2):62-63,91.
[2]陶淑霞,李玉,刘家富,等.球孢白僵菌对亚洲玉米螟幼虫血细胞数量和孢囊作用的影响[J].植物保护学报,2011,38(6):527-531.
[3]魏灵燕.不同白僵菌菌株的生物学特性比较及HFW-05菌株田间种群动态[D].保定:河北农业大学,2012.
[4]汤坚,黄长春,丁珊,等.球孢白僵菌制剂载体的筛选[J].安徽农业大学学报,1996(3):351-354.
[5]田甜.飞蝗优良绿僵菌、白僵菌菌株的筛选及应用性研究[D].保定:河北农业大学,2009.
[6]刘健,陈洪章,李佐虎.白僵菌杀虫剂生产工艺研究状况与展望[J].中国生物防治,2003,19(2):86-90.
[7]朱昌雄,丁振华,蒋细良,等.微生物农药剂型研究发展趋势[J].现代化工,2003,23(3):4-8.
[8]殷凤鸣,潘务耀,李增智.白僵菌生产企业标准[J].安徽农业大学学报,1996(23):321-3251.
[9]Feng M G,T J Poprawski.Robustness of the time-dose-mortality model in bioassay data analysis of microbial control agents and chemical agents for insect control[J].Subtropical Plant Science,1999(51):36-38.
[10]刘兴磊.球孢白僵菌的生物学特性及对蚜虫的致病力研究[D].哈尔滨:东北农业大学,2011.
[11]彭国雄,张永军,杨星勇,等.球孢白僵菌不同世代菌株胞外蛋白酶与毒力的关系[J].中国生物防治,2000,16(2):61-64.
[12]程国华,舒静,丁克坚.球孢白僵菌营养需求及培养条件研究[J].中国农学通报,2006(5):365-368.
[13]张航,崔阳,董晓宇,等.球孢白僵菌孢子的耐逆性研究[J].哈尔滨师范大学自然科学学报,2014,30(5):104-107.
[14]应盛华,冯明光.真空干燥球孢白僵菌纯孢粉的活孢率、毒力与贮存期[J].微生物学通报,2002,29(5):42-47.
[15]王义生,朱晓敏,张荣宝,等.5种除草剂对白僵菌孢子萌发的影响[J].吉林农业科学,2015,40(2):62-63,91.