TLR4基因在三黄鸡不同器官中的表达规律分析
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摘要
为探究TLR4基因在三黄鸡不同器官中的表达规律,试验通过运用real-time PCR方法检测TLR4基因在100日龄三黄鸡公、母鸡不同器官中的表达情况。结果显示:三黄鸡母鸡的法氏囊、肌肉、肺、心、肝、下丘脑中TLR4的表达水平最高,脾脏、十二指肠中TLR4的表达水平较高,腺胃、胸腺、卵巢、回肠、肌胃、垂体、肾、盲肠、空肠和大脑最低。三黄鸡公鸡的心、胸腺、下丘脑、肺、法氏囊、垂体、肌肉、肝、脾中TLR4的表达水平显著高于其他器官。而公、母鸡对比,公鸡胸腺中TLR4的表达水平显著低于母鸡,但是法氏囊中TLR4的表达水平显著高于母鸡。研究结果为TLR4的深入研究提供了理论基础,同时为三黄鸡的抗病育种提供新思路。
In order to explore the expression of TLR4 gene in different organs of Sanhuang chicken, real-time PCR was used to detect the expression of TLR4 gene in different organs of 100-day-old Sanhuang rooster and hen. The results showed that expression levels of TLR4 gene of Sanhuang hen in bursa, muscle, lung, heart, liver and hypothalamus were higher than that in other organs, while the expression levels of TLR4 gene in spleen and duodenum were higher than that in glandular stomach, thymus, ovary, ileum, muscle stomach, pituitary, kidney, cecum, jejunum and brain.The expression levels of TLR4 of Sanhuang rooster in the heart, thymus, hypothalamus, lung, bursa, pituitary, muscle,liver and spleen were significantly higher than that in other organs. Compared with roosters and hens, the expression level of TLR4 in rooster′s thymus was significantly lower than that in hens, but the expression level of TLR4 in rooster′s bursa was significantly higher than that in hens. These results provide a theoretical basis for the in-depth study of TLR4, and provide new ideas for disease resistance breeding of Sanhuang chicken.
In order to explore the expression of TLR4 gene in different organs of Sanhuang chicken, real-time PCR was used to detect the expression of TLR4 gene in different organs of 100-day-old Sanhuang rooster and hen. The results showed that expression levels of TLR4 gene of Sanhuang hen in bursa, muscle, lung, heart, liver and hypothalamus were higher than that in other organs, while the expression levels of TLR4 gene in spleen and duodenum were higher than that in glandular stomach, thymus, ovary, ileum, muscle stomach, pituitary, kidney, cecum, jejunum and brain.The expression levels of TLR4 of Sanhuang rooster in the heart, thymus, hypothalamus, lung, bursa, pituitary, muscle,liver and spleen were significantly higher than that in other organs. Compared with roosters and hens, the expression level of TLR4 in rooster′s thymus was significantly lower than that in hens, but the expression level of TLR4 in rooster′s bursa was significantly higher than that in hens. These results provide a theoretical basis for the in-depth study of TLR4, and provide new ideas for disease resistance breeding of Sanhuang chicken.
引文
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[23] NIHASHI Y, ONO T, KAGAMI H, et al. Toll-like receptor ligand-dependent inflammatory responses in chick skeletal muscle myoblasts[J]. Dev Comp Immunol, 2019(91):115-122.
[2] YANG R, SONG Z, WU S, et al. Toll-like receptor 4contributes to a myofibroblast phenotype in cardiac fibroblasts and is associated with autophagy after myocardial infarction in a mouse model[J]. Atherosclerosis, 2018(279):23-31
[3] BACHAR O, ADNER M, UDDMAN R, et al. Toll-like receptor stimulation induces airway hyper-responsiveness to bradykinin, an effect mediated by JNK and NF-kappa B signaling pathways[J]. Eur J Immunol, 2004, 34(4):1196-1207.
[4] PAN Q, ZHANG Q, CHU J, et al. Pmp18.1 Induces IL-1βSecretion by TLR4 Activation through the MyD88, NF-κB, and Caspase-1 Signaling Pathways[J]. Front Cell Infect Microbiol, 2017(7):514.
[5] WANG F X, YANG X L, MA Y S, et al. TRIF contributes to epileptogenesis in temporal lobe epilepsy during TLR4 activation[J]. Brain Behav Immun, 2018(67):65-76.
[6] LAI M Y, YAO H, SHAH S Z A, et al. The NLRP3-Caspase 1 Inflammasome Negatively Regulates Autophagy via TLR4-TRIF in Prion Peptide-Infected Microglia[J]. Front Aging Neurosci, 2018(10):116.
[7] CHENG P, WANG T, LI W, et al. Baicalin Alleviates Lipopolysaccharide-Induced Liver Inflammation in Chicken by Suppressing TLR4-Mediated NF-κB Pathway[J]. Front Pharmacol, 2017(8):547.
[8] MENG Y Y, LIU Y H, ZHE F, et al. Sanguinarine Attenuates Lipopolysaccharide-induced Inflammation and Apoptosis by Inhibiting the TLR4/NF-κB Pathway in H9c2Cardiomyocytes[J]. Curr Med, 2018, 38(2):204-211.
[9] WANG T, OUYANG H, ZHOU H, et al. Pro atherogenic activation of A7r5 cells induced by the oxLDL/β2GPI/antiβ2GPI complex[J].Int J Mol Med, 2018, 42(4):1955-1966.
[10] WU K L, ZHANG H H, FU Y J, et al. TLR4/MyD88 signaling determines the metastatic potential of breast cancer cells[J]. Mol Med Rep, 2018, 18(3):3411-3420.
[11] WANG Q W, SU Y, SHENG J T, et al. Anti-influenza A virus activity of rhein through regulating oxidative stress,TLR4, Akt, MAPK, and NF-κB signal pathways[J]. PLoS One, 2018, 13(1):e0191793.
[12]徐文娟,陶志云,朱春红,等.金定鸭不同组织和血液中TLR1、TLR2、TLR4、TLR5的表达差异分析[J].中国畜牧兽医,2017,44(12):3459-3465.
[13]林宝山,兰道亮,黄偲,等.牦牛TLR2和TLR4基因mRNA在不同组织中的表达分布研究[J].中国预防兽医学报,2014,36(11):901-903.
[14]刘艳.鸡TLR4和Mx基因遗传变异及其表达规律分析[D].扬州:扬州大学,2011.
[15]杜晓辉,李荣,姚咏明,等.性别差异对脓毒症大鼠心肌Toll样受体4和髓样分化蛋白-2基因表达的影响[J].军医进修学院学报,2006(3):196-198.
[16]聂明权.鸡传染性法氏囊病的诊治[J].畜牧兽医科技信息,2017(7):97-98.
[17]王云.鸡胸腺发育形态学研究[D].武汉:华中农业大学,2010.
[18] EREN U K S, NAZLIGUL A. The several elements of intestinal innate immune system at the beginning of the life of broiler chicks[J]. Microsc Res, Tech, 2016(79):604-614.
[19] XU B, ZANG S, LI S, et al. HMGB1-mediated differential response on hippocampal neurotransmitter disorder and neuroinflammation in adolescent male and female mice following cold exposure[J]. Brain Behav Immun,2018.
[20]康喜龙,唐娟,焦扬,等.家禽沙门菌免疫学研究进展[J].中国家禽,2018,40(21):1-6.
[21] MANN D L. The Emerging Role of Innate Immunity in the Heart and Vascular System:For Whom the Cell Tolls[J].Circulation Research, 2011, 108(9):1133-1145.
[22] POLLACK A, KONTOROVICH A R, FUSTER V, et al.Viral myocarditis—diagnosis, treatment options, and current controversies[J]. Nature Reviews Cardiology, 2015, 12(11):670-80.
[23] NIHASHI Y, ONO T, KAGAMI H, et al. Toll-like receptor ligand-dependent inflammatory responses in chick skeletal muscle myoblasts[J]. Dev Comp Immunol, 2019(91):115-122.