胶体金修饰电极测定鱼样中组胺的方法研究
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摘要
基于胶体金修饰的玻碳电极,利用电流～时间曲线法建立了一种简便、灵敏的组胺检测方法。优化了底液的pH值和组胺的电化学测试方法及条件,考察了修饰电极的电化学性能。结果表明,组胺在胶体金修饰电极上的响应电流(-I,μA)与其浓度(c,μmol/L)在0. 1～64μmol/L范围内呈良好的线性关系,检出限为0. 033μmol/L,在带鱼和黄花鱼样品中的加标回收率分别为94. 4%～106%、91. 8%～106%,相对标准偏差分别为3. 2%、2. 5%。该法操作简单、检测速度快、成本低,适用于带鱼和黄花鱼等鱼样中组胺的测定。
A simple and sensitive method was established for the detection of histamine by using the current-time curve based on a colloidal gold modified glass carbon electrode( AuCS/GCE). The electrochemical properties of the modified electrode were investigated. The pH value of the base solution,and the electrochemical detection methods and conditions for histamine were optimized. Results showed that there was a good linear relationship for the response current(-I,μA) of histamine on the Au CS/GCE in the concentration( c,μmol/L) range of 0. 1-64 μmol/L,with the detection limit of 0. 033 μmol/L. The recoveries for histamine in the hairtail and yellow croaker samples were in the ranges of 94. 4%-106% and 91. 8%-106%,respectively. The method was simple,rapid and low cost,and was suitable for the determination of histamine in food samples such as hairtail and yellow croaker.
A simple and sensitive method was established for the detection of histamine by using the current-time curve based on a colloidal gold modified glass carbon electrode( AuCS/GCE). The electrochemical properties of the modified electrode were investigated. The pH value of the base solution,and the electrochemical detection methods and conditions for histamine were optimized. Results showed that there was a good linear relationship for the response current(-I,μA) of histamine on the Au CS/GCE in the concentration( c,μmol/L) range of 0. 1-64 μmol/L,with the detection limit of 0. 033 μmol/L. The recoveries for histamine in the hairtail and yellow croaker samples were in the ranges of 94. 4%-106% and 91. 8%-106%,respectively. The method was simple,rapid and low cost,and was suitable for the determination of histamine in food samples such as hairtail and yellow croaker.
引文
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[19] Guo H. Biogenic Amines Profile and Characteristic Amines Analysis at Different Storage Temperature in Marine Fishes.Hangzhou:Zhejiang University(郭慧.不同贮藏温度条件下海洋鱼类生物胺变化规律研究及特征生物胺分析.杭州:浙江大学),2015.
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[21] Hu Y. Determination of Biogenic Amines and Isolation of Histamine-producing Bacteria in Aquatic Foods. Xiamen:JimeiUniversity(胡月.水产品中生物胺的测定及产组胺菌的分离与鉴定.厦门:集美大学),2013.
[22] Guo H,Liu X Q,Chao L P,Jiang Y H. J. Chin. Inst. Food Sci. Technol.(郭慧,刘晓琴,晁鲁平,蒋益虹.中国食品学报),2017,17(5):194-199.
[2] Apetrei I M,Apetrei C. Sens. Actuators B,2013,178:40-46.
[3] Spano G,Russo P,Lonvaud-Funel A,Lucas P,Alexandre H,Grandvalet C,Coton E,Coton M,Barnavon L,BachB,Rattray F,Bunte A,Magni C,Ladero V,Alvarez M,Fernández M,Lopez P,Palencia P F,Corbi A,Trip H,Lolkema J S. Eur. J. Clin. Nutr.,2010,64:95-100.
[4] Preti R,Antonelli M L,Bernacchia R,Vinci G. Food Chem.,2015,187:555-562.
[5] de Borba B M,Rohrer J S. J. Chromatogr. A,2007,1155(1):22-30.
[6] Al Bulushi I,Poole S,Deeth H C,Dykes G A. Crit. Rev. Food Sci.,2009,49:369-377.
[7] Apetrei I M,Apetrei C. J. Food Eng.,2015,149:1-8.
[8] Lieberman P. Ann. Allerg. Asthma Immunol.,2011,106(2):2-5.
[9] Takagi K,Shikata S. Anal. Chim. Acta,2004,505(2):189-193.
[10] Panula P,Sundvik M,Karlstedt K. Trends Neurosci.,2014,37(3):195-168.
[11] Feng C,Teuber S,Gershwin M E. Clin. Rev. Allerg. Immunol.,2016,50(1):64-69.
[12] Veseli A,Vasjari M,Arbneshi T,Hajrizi A,Svorc L,Samphao A,Kalcher K. Sens. Actuators B,2016,228:774-781.
[13] Yan P P. Fabrication and Application of Electrochemiluminescent Immunosensor Based on Quantum Dots and Gold Nanopar-ticles. Suzhou:Soochow University(闫盼盼.基于量子点和胶体金电化学发光免疫传感器的构建及应用.苏州:苏州大学),2014.
[14] Wang Y,Jiao T F,Xie D Y,Wang F Y. Inorg. Anal. Chem. Chin.(王莹,焦体峰,谢丹阳,王风彦.中国无机分析化学),2012,2(4):15-21.
[15] Bai W Q,Huang H Y,Li Y,Zhang H Y,Liang B,Guo R,Du L L,Zhang Z W. Electrochim. Acta,2014,(117):322-328.
[16] Wang Y S. Design and Applications Research of Au Nanoparticles Composites. Changchun:Jilin University(王英帅.金纳米粒子复合材料的设计及应用研究.长春:吉林大学),2017.
[17] Yi L J. Research and Application on the Electrochemical Detection Methods of Tyramine and Tryptamine in FermentedFoods. Guangzhou:Zhongkai University of Agriculture and Engineering(易丽娟.发酵食品中酪胺和色胺的电化学检测方法研究与应用.广州:仲恺农业工程学院),2017.
[18] Zhang Y. Ultra-high Performance Liquid Chromatography Detection Technology on the Variation of Biogenic Amines in A-quatic Products. Nanjing:Nanjing Agricultural University(张阳.水产品中生物胺含量检测方法的建立及其变化规律的研究.南京:南京农业大学),2012.
[19] Guo H. Biogenic Amines Profile and Characteristic Amines Analysis at Different Storage Temperature in Marine Fishes.Hangzhou:Zhejiang University(郭慧.不同贮藏温度条件下海洋鱼类生物胺变化规律研究及特征生物胺分析.杭州:浙江大学),2015.
[20] Wu Y Y,Chen Y F,Li L H,Yang X Q,Lin W L,Yang S L,Wang J X. J. Fish. Chin.(吴燕燕,陈玉峰,李来好,杨贤庆,林婉玲,杨少玲,王锦旭.水产学报),2015,39(10):1577-1586.
[21] Hu Y. Determination of Biogenic Amines and Isolation of Histamine-producing Bacteria in Aquatic Foods. Xiamen:JimeiUniversity(胡月.水产品中生物胺的测定及产组胺菌的分离与鉴定.厦门:集美大学),2013.
[22] Guo H,Liu X Q,Chao L P,Jiang Y H. J. Chin. Inst. Food Sci. Technol.(郭慧,刘晓琴,晁鲁平,蒋益虹.中国食品学报),2017,17(5):194-199.