‘降糖稻1号’穗部不同部位籽粒抗性淀粉含量的差异分析
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摘要
以上海市农业科学院作物育种栽培研究所培育的高抗性淀粉(Resistant starch,RS)水稻品系‘降糖稻1号’为研究材料,对其不同部位籽粒RS含量进行了分析。结果表明:‘降糖稻1号’穗部RS含量与粒重因部位和粒位的不同而存在差异。具体表现为:同一部位RS含量和粒重一次枝梗高于二次枝梗,且均表现为穗上部较高、穗下部较低;同一枝梗上不同粒位间RS含量和粒重也均呈现顶部粒位相对较高、基部粒位相对较低这一基本趋势,并且相同粒位上一次枝梗的RS含量和粒重均高于二次枝梗;有胚侧半粒米RS含量比无胚侧半粒米RS含量高;随着碾磨时间的延长,RS含量有逐渐提高的趋势,即米粒胚乳表层的RS含量最低,心部的RS含量最高。该研究为高RS新品种的选育以及RS含量的正确测定提供了依据。
The rice line 'Jiangtangdao 1' bred by this institute was used as a test material,and its resistantstarch(RS)contents of grains in different parts of panicles were analyzed. The results showed that the RS contentand grain weight of ‘ Jiangtangdao 1' were various in different parts of a rice panicle or grains. In the samespikelet position both RS content and grain weight of the primary rachis-branch were higher than those of thesecondary rachis-branch,and both were higher in the upper panicle than in the lower panicle; Of the samerachis-branch both RS content and grain weight were also higher at the top than at the bottom,and in the samegrain position both were higher in the primary rachis-branch than in the secondary rachis-branch; The half grainwith embryo had a higher RS content than that without embryo; The RS content increased gradually as themilling time prolonged,indicating that the RS content was the lowest in the grains' skin endosperm layer and thehighest in the grains' endosperm center. The above results provided a theoretical basis for breeding a new high-RS rice variety and accurately determining the RS content.
The rice line 'Jiangtangdao 1' bred by this institute was used as a test material,and its resistantstarch(RS)contents of grains in different parts of panicles were analyzed. The results showed that the RS contentand grain weight of ‘ Jiangtangdao 1' were various in different parts of a rice panicle or grains. In the samespikelet position both RS content and grain weight of the primary rachis-branch were higher than those of thesecondary rachis-branch,and both were higher in the upper panicle than in the lower panicle; Of the samerachis-branch both RS content and grain weight were also higher at the top than at the bottom,and in the samegrain position both were higher in the primary rachis-branch than in the secondary rachis-branch; The half grainwith embryo had a higher RS content than that without embryo; The RS content increased gradually as themilling time prolonged,indicating that the RS content was the lowest in the grains' skin endosperm layer and thehighest in the grains' endosperm center. The above results provided a theoretical basis for breeding a new high-RS rice variety and accurately determining the RS content.
引文
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[12]庞欢,李卫华,张宏斌,等.小麦籽粒抗性淀粉含量的分析[J].遗传,2010,32(2):170-176.
[13]赵凯,张守文,方桂珍.抗性淀粉的生理功能及在食品工业中的应用[J].哈尔滨商业大学学报(自然科学版),2009(6):661-663.
[14]杨瑞芳,白建江,方军,等.分子标记辅助选择选育高抗性淀粉水稻新品种[J].核农学报,2015,29(12):2259-2267.
[15]MAKI K C,PELKMAN C L,FINOCCHIARO E T,et al.Resistant starch from high-amylose maize increases insulin sensitivity in overweight and obese men[J].Journal of Nutrition,2012,142(4):717-723.
[16]张宁,孙健,熊海铮,等.高抗性淀粉含量糖尿病专用粳稻的选育及其特征特性[J].中国稻米,2011,17(6):63-65.
[17]罗曦,黄锦峰,朱永生,等.水稻功米3号高抗性淀粉性状的遗传分析[J].农业生物技术学报,2014,22(1):10-16.
[18]牟方贵,闫宗武,冉瑞林,等.水稻抗性淀粉相关SSR标记的初步研究[J].分子植物育种,2008,6(3):432-438.
[19]YANG R F,SUN C L,BAI J J,et al.A putative gene sbe3-rs for resistant starch mutated from SBE3 for starch branching enzyme in rice(Oryza sativaL.)[J].Plo S one,2012,7(8):e43026.
[20]SHU X L,SUN J,WU D X.Effects of grain development on formation of resistant starch in rice[J].Food Chemistry,2014,164(3):89-97.
[21]董明辉,桑大志,王朋,等.水稻穗上不同部位籽粒垩白性状的差异[J].作物学报,2006,32(1):103-111.
[22]孟维韧,王伯伦,吕军,等.水稻着粒密度对产量和品质形成的影响[J].华中农业大学学报,2009,28(3):262-267.
[23]杨树明,夏小环,谢勇武,等.散穗与密穗型水稻穗上不同部位籽粒的粒重及抗性淀粉含量[J].湖南农业大学学报(自然科学版),2011,37(2):115-119.
[24]朱海江,程方民,王丰,等.两种穗型粳稻穗内粒间直链淀粉含量变异与粒位分布特征[J].中国水稻科学,2004,18(4):321-325.
[25]陆艳婷,张小明,叶胜海,等.密穗型和散穗型粳稻穗部不同部位直链淀粉含量的差异[J].中国水稻科学,2007,21(4):391-395.
[26]金峰,陈书强,徐正进,等.直立与弯曲穗型水稻穗上不同部位籽粒碾磨品质的比较[J].中国水稻科学,2008,22(2):167-174.
[2]KESTELL P,ZHU S,FERGUSON L R.Mechanisms by which resistant starches and non-starch polysaccharide sources affect the metabolism and disposition of the food carcinogen,2-amino-3-methylimidazo[J].Journal of Chromatography B,2004,802(1):201-210.
[3]DE DECKERE E A,KLOOTS W J,VAN AMELSVCORT J M.Resistant starch decreases serums total cholesterol and triacylglycerol concentration in rats[J].American Institute of Nutrition,1993,7:2142-2151.
[4]RANHOTRA G S,GELROTH J A,GLASER B K.Energy value of resistant starch[J].Journal of Food Science,2010,61(2):453-455.
[5]KIM W K,CHUNG M K,KANG N E,et al.Effect of resistant starch from corn or rice on glucose control,colonic events,and blood lipid concentrations in streptozotocin-induced diabetic rats[J].Journal of Nutritional Biochemistry,2003,14(3):166-172.
[6]ROSIN P M,LAJOLO F M,MENEZES E W.Measurement and characterization of dietary starches[J].Journal of Food Composition&Analysis,2002,15(4):367-377.
[7]CROSBY G A.Resistant starch makes better carbs[J].Funct Foods&Nutrace,2003,6:34-36.
[8]朱辉明,白建江,王慧,等.抗生素对抗性淀粉含量测定值的影响[J].上海农业学报,2010,26(2):58-60.
[9]朱辉明,白建江,王慧,等.高抗性淀粉粳稻新品系稻米淀粉特性[J].中国农学通报,2010,26(14):108-112.
[10]张淑梅,张建明,李丁鲁,等.高抗性淀粉粳稻品系稻米淀粉链长分布与主要品质特征差异[J].中国农业科学,2009,42(6):2237-2243.
[11]焦桂爱,唐绍清,罗炬,等.水稻抗性淀粉突变体抗性淀粉结构的比较研究[J].中国水稻科学,2006,20(6):645-648.
[12]庞欢,李卫华,张宏斌,等.小麦籽粒抗性淀粉含量的分析[J].遗传,2010,32(2):170-176.
[13]赵凯,张守文,方桂珍.抗性淀粉的生理功能及在食品工业中的应用[J].哈尔滨商业大学学报(自然科学版),2009(6):661-663.
[14]杨瑞芳,白建江,方军,等.分子标记辅助选择选育高抗性淀粉水稻新品种[J].核农学报,2015,29(12):2259-2267.
[15]MAKI K C,PELKMAN C L,FINOCCHIARO E T,et al.Resistant starch from high-amylose maize increases insulin sensitivity in overweight and obese men[J].Journal of Nutrition,2012,142(4):717-723.
[16]张宁,孙健,熊海铮,等.高抗性淀粉含量糖尿病专用粳稻的选育及其特征特性[J].中国稻米,2011,17(6):63-65.
[17]罗曦,黄锦峰,朱永生,等.水稻功米3号高抗性淀粉性状的遗传分析[J].农业生物技术学报,2014,22(1):10-16.
[18]牟方贵,闫宗武,冉瑞林,等.水稻抗性淀粉相关SSR标记的初步研究[J].分子植物育种,2008,6(3):432-438.
[19]YANG R F,SUN C L,BAI J J,et al.A putative gene sbe3-rs for resistant starch mutated from SBE3 for starch branching enzyme in rice(Oryza sativaL.)[J].Plo S one,2012,7(8):e43026.
[20]SHU X L,SUN J,WU D X.Effects of grain development on formation of resistant starch in rice[J].Food Chemistry,2014,164(3):89-97.
[21]董明辉,桑大志,王朋,等.水稻穗上不同部位籽粒垩白性状的差异[J].作物学报,2006,32(1):103-111.
[22]孟维韧,王伯伦,吕军,等.水稻着粒密度对产量和品质形成的影响[J].华中农业大学学报,2009,28(3):262-267.
[23]杨树明,夏小环,谢勇武,等.散穗与密穗型水稻穗上不同部位籽粒的粒重及抗性淀粉含量[J].湖南农业大学学报(自然科学版),2011,37(2):115-119.
[24]朱海江,程方民,王丰,等.两种穗型粳稻穗内粒间直链淀粉含量变异与粒位分布特征[J].中国水稻科学,2004,18(4):321-325.
[25]陆艳婷,张小明,叶胜海,等.密穗型和散穗型粳稻穗部不同部位直链淀粉含量的差异[J].中国水稻科学,2007,21(4):391-395.
[26]金峰,陈书强,徐正进,等.直立与弯曲穗型水稻穗上不同部位籽粒碾磨品质的比较[J].中国水稻科学,2008,22(2):167-174.