黑龙江省建三江地区主栽粳稻DNA指纹图谱构建研究
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摘要
利用农业行业标准(NY/T1433-2007)推荐的12对引物,采用简单重复系列(Simple Sequence Repeats,SSR)标记和毛细管电泳方法,对黑龙江省建三江地区的12个主栽粳稻品种进行检测,构建DNA指纹图谱和品种的分子身份证。12对引物在12份品种中共检测到30个等位基因,每对引物检测到的等位基因数平均值为2.5个。根据引物在水稻品种中的扩增和毛细管电泳测定的结果,采用0/1矩阵构建由30位数字组成的DNA指纹图谱。分子身份证包括品种本身信息及SSR指纹信息,通过软件生成水稻品种身份证条形码和二维码,为水稻品种的鉴定及溯源管理提供技术支撑。
To construct DNA fingerprints and the molecular identity cards of 12 major varieties japonica rice in Jiansanjiang area of Heilongjiang province,12 primers recommended by the national standard(NY/T 1433-2007)were detected by using the simple sequence repeat(SSR)technique and capillary electrophoresis.The results showed that:30 alleles were detected in the 12 varieties using the 12 primer pairs.The number of alleles detected by each primer pair was an average of 2.5.According to the amplification results obtained by using the rice primers and capillary electrophoresis in rice varieties,a 0/1 matrix was used to construct the DNA fingerprints consisting of 30 digits.The molecular ID cards included variety information and SSR fingerprint information,formed barcode and QR code of rice variety ID card through software,which provided technical support for the identification and traceability management of rice varieties.
To construct DNA fingerprints and the molecular identity cards of 12 major varieties japonica rice in Jiansanjiang area of Heilongjiang province,12 primers recommended by the national standard(NY/T 1433-2007)were detected by using the simple sequence repeat(SSR)technique and capillary electrophoresis.The results showed that:30 alleles were detected in the 12 varieties using the 12 primer pairs.The number of alleles detected by each primer pair was an average of 2.5.According to the amplification results obtained by using the rice primers and capillary electrophoresis in rice varieties,a 0/1 matrix was used to construct the DNA fingerprints consisting of 30 digits.The molecular ID cards included variety information and SSR fingerprint information,formed barcode and QR code of rice variety ID card through software,which provided technical support for the identification and traceability management of rice varieties.
引文
[1]王滨.中国绿色米都建三江[J].黑龙江粮食,2014(12):44-46.
[2]宋雪健,冷侯喜,张爱武,等.黑龙江省不同产地大米中矿物元素含量分析与比较[J].黑龙江八一农垦大学学报,2016,28(3):66-70.
[3]陆徐忠,从夕汉,刘海珍,等.杂交水稻亲本分子身份证及SSR指纹数据库的建立[J].核农学报,2012,26(6):853-861.
[4]李茂柏,王慧,白建江,等.利用SSR分子标记构建水稻品种DNA指纹图谱的研究进展[J].中国稻米,2011,17(1):4-6.
[5]李莉,王俊峰,颜廷进,等.基于SSR标记的山东省小麦DNA指纹图谱的构建[J].植物遗传资源学报,2013,14(3):537-541.
[6]王黎明,焦少杰,姜艳喜,等.142份甜高粱品种的分子身份证构建[J].作物学报,2011,37(11):1975-1983.
[7]高运来,姚丙晨,刘春燕,等.黑龙江省主栽大豆品种遗传多样性的SSR分析[J].植物学报,2009,44(5):556-561.
[8]王凤格,杨扬,易红梅,等.中国玉米审定品种标准SSR指纹库的构建[J].中国农业科学,2017,50(1):1-14.
[9]肖小余,王玉平,张建勇,等.四川省主要杂交稻亲本的SSR多态性分析和指纹图谱的构建与应用[J].中国水稻科学,2006,20(1):1-7.
[10]应杰政,施勇烽,庄杰云,等.用微卫星标记评估中国水稻主栽品种的遗传多样性[J].中国农业科学,2007,40(4):649-654.
[11]李小华,叶胜海,赵小燕,等.浙江省46个粳稻新品种SSR指纹图谱构建及遗传多样性分析[J].浙江农业学报,2014,26(5):1158-1163.
[12]Chakravarthi B K,Naravaneni R.SSR marker based DNAfingerprinting and diversity study in rice(Oryza sativa.L)[J].African Journal of Biotechnology,2006(9):684-688.
[13]Sarao N K,Vikal Y,Singh K,et al.SSR marker-based DNA fingerprinting and cultivar identification of rice(Oryza sativa L.)in Punjab state of India[J].Plant Genetic Resources,2010,8(1):42-44.
[14]NYT 1433-2007.水稻品种鉴定DNA指纹方法[S].北京:中华人民共和国农业部,2007.
[15]程本义,夏俊辉,龚俊义,等.SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用[J].中国水稻科学,2011,25(6):672-676.
[16]董亚蕾,陈晓姣,胡敬,等.高效毛细管电泳在食品安全检测中的应用进展[J].色谱,2012,30(11):1117-1126.
[17]Doyle J J A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochem Bull,1987(19):11-15.
[18]徐世龙,严天泽,钟日超,等.基于SSR标记的中籼型两系杂交水稻亲本遗传差异分析[J].杂交水稻,2017,32(3):79-84.
[19]吴帅,雷声,杨锡洪,等.指纹分析技术及其在食品中的应用[J].食品与机械,2015(1):249-252.
[20]Iudith I,Bogdan A,János S,et al.Genetic Fingerprint-Innovative Method for Animal Products Traceability in the Context of Bio-economy[J].Procedia Economics&Finance,2013(8):414-419.
[21]宋丰顺,倪大虎,倪金龙,等.杂交水稻种子纯度检测方法综述[J].江苏农业科学,2016,44(6):6-11.
[22]陆徐忠,倪金龙,李莉,等.利用SSR分子指纹和商品信息构建水稻品种身份证[J].作物学报,2014,40(5):823-829.
[23]王黎明,焦少杰,姜艳喜,等.142份甜高粱品种的分子身份证构建[J].作物学报,2011,37(11):1975-1983.
[2]宋雪健,冷侯喜,张爱武,等.黑龙江省不同产地大米中矿物元素含量分析与比较[J].黑龙江八一农垦大学学报,2016,28(3):66-70.
[3]陆徐忠,从夕汉,刘海珍,等.杂交水稻亲本分子身份证及SSR指纹数据库的建立[J].核农学报,2012,26(6):853-861.
[4]李茂柏,王慧,白建江,等.利用SSR分子标记构建水稻品种DNA指纹图谱的研究进展[J].中国稻米,2011,17(1):4-6.
[5]李莉,王俊峰,颜廷进,等.基于SSR标记的山东省小麦DNA指纹图谱的构建[J].植物遗传资源学报,2013,14(3):537-541.
[6]王黎明,焦少杰,姜艳喜,等.142份甜高粱品种的分子身份证构建[J].作物学报,2011,37(11):1975-1983.
[7]高运来,姚丙晨,刘春燕,等.黑龙江省主栽大豆品种遗传多样性的SSR分析[J].植物学报,2009,44(5):556-561.
[8]王凤格,杨扬,易红梅,等.中国玉米审定品种标准SSR指纹库的构建[J].中国农业科学,2017,50(1):1-14.
[9]肖小余,王玉平,张建勇,等.四川省主要杂交稻亲本的SSR多态性分析和指纹图谱的构建与应用[J].中国水稻科学,2006,20(1):1-7.
[10]应杰政,施勇烽,庄杰云,等.用微卫星标记评估中国水稻主栽品种的遗传多样性[J].中国农业科学,2007,40(4):649-654.
[11]李小华,叶胜海,赵小燕,等.浙江省46个粳稻新品种SSR指纹图谱构建及遗传多样性分析[J].浙江农业学报,2014,26(5):1158-1163.
[12]Chakravarthi B K,Naravaneni R.SSR marker based DNAfingerprinting and diversity study in rice(Oryza sativa.L)[J].African Journal of Biotechnology,2006(9):684-688.
[13]Sarao N K,Vikal Y,Singh K,et al.SSR marker-based DNA fingerprinting and cultivar identification of rice(Oryza sativa L.)in Punjab state of India[J].Plant Genetic Resources,2010,8(1):42-44.
[14]NYT 1433-2007.水稻品种鉴定DNA指纹方法[S].北京:中华人民共和国农业部,2007.
[15]程本义,夏俊辉,龚俊义,等.SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用[J].中国水稻科学,2011,25(6):672-676.
[16]董亚蕾,陈晓姣,胡敬,等.高效毛细管电泳在食品安全检测中的应用进展[J].色谱,2012,30(11):1117-1126.
[17]Doyle J J A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochem Bull,1987(19):11-15.
[18]徐世龙,严天泽,钟日超,等.基于SSR标记的中籼型两系杂交水稻亲本遗传差异分析[J].杂交水稻,2017,32(3):79-84.
[19]吴帅,雷声,杨锡洪,等.指纹分析技术及其在食品中的应用[J].食品与机械,2015(1):249-252.
[20]Iudith I,Bogdan A,János S,et al.Genetic Fingerprint-Innovative Method for Animal Products Traceability in the Context of Bio-economy[J].Procedia Economics&Finance,2013(8):414-419.
[21]宋丰顺,倪大虎,倪金龙,等.杂交水稻种子纯度检测方法综述[J].江苏农业科学,2016,44(6):6-11.
[22]陆徐忠,倪金龙,李莉,等.利用SSR分子指纹和商品信息构建水稻品种身份证[J].作物学报,2014,40(5):823-829.
[23]王黎明,焦少杰,姜艳喜,等.142份甜高粱品种的分子身份证构建[J].作物学报,2011,37(11):1975-1983.