玉米低磷胁迫诱导型启动子P_(1502)-ZmPHR1在玉米中的瞬时表达
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摘要
用农杆菌介导法将玉米低磷胁迫诱导型启动子P_(1502)-ZmPHR1与GUS报告基因的融合基因分别转入玉米478未成熟幼胚以及萌动的成熟胚中,通过检测GUS基因的瞬时表达情况来分析该启动子在玉米中的表达活性。结果表明,P_(1502)-ZmPHR1在玉米中低磷条件下被诱导表达,具有启动活性。
The fusion gene of strong inducible promoter under phosphrous stress P_(1502)-ZmPHR1 in maize and GUS reporter gene was transferred into maize inbreed line 478 immature embryos and mature embryos by Agrobacterium tumefaciens mediated method.Then the transient expression of GUS gene was detected to analyze the expression activity of the promoter in maize. The results showed that P_(1502)-ZmPHR1 could be induced and expressed under phosphrous stress conditions in maize.
The fusion gene of strong inducible promoter under phosphrous stress P_(1502)-ZmPHR1 in maize and GUS reporter gene was transferred into maize inbreed line 478 immature embryos and mature embryos by Agrobacterium tumefaciens mediated method.Then the transient expression of GUS gene was detected to analyze the expression activity of the promoter in maize. The results showed that P_(1502)-ZmPHR1 could be induced and expressed under phosphrous stress conditions in maize.
引文
[1]HELLENS R P,ALLAN A C,FRIEL E N,et al.Transient expression vectors for functional genomics,quantification of promoter activity and RNA silencing in plants[J].Plant Methods,2005,1(1):13.
[2]LEE MW,YANG Y.Transient expression vectors for functional genomics,quantification of promoter activity and RNA silencing in plan[J].Methods in Molecular Biology,2006,323:225-229.
[3]KEARNS A,HAWES C,SPARKES I A,et al.Rapid,transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants[J].Nature Protocols,2006,1(4):2019-2025.
[4]邱礽,陶刚,李奇科,等.农杆菌渗入法介导的基因瞬时表达技术及应用[J].分子植物育种,2009(5):1032-1039.
[5]BA譙UELOS MA,GARCIADEBLAS B,CUBERO B,et al.Inventory and functional characterization of the HAK potassium transporters of rice[J].Plant Physiology,2002,130(2):784-795.
[6]刘雪梅,王蕾,文添龙,等.农杆菌介导的棉花子叶瞬时表达系统的建立[J].植物学报,2014(5):587-594.
[7]JENNINGS D H.Mineral nutrition in higher plants(book)[J].Plant Cell&Environment,1988,11(2):147.
[8]RAGHOTHAMA K G,KARTHIKEYAN A S,LAMBERS H,et al.Phosphate acquisition[J].Plant&Soil,2005,274(1/2):37-49.
[9]SMITH F W,MUDGE S R,RAE A L,et al.Phosphate transport in plants[J].Plant&Soil,2003,248(1/2):71-83.
[10]WANG X,BAI J,LIU H,et al.Overexpression of a maize transcription factor Zm PHR1,improves shoot inorganic phosphate content and growth of Arabidopsis,under low-phosphate conditions[J].Plant Molecular Biology Reporter,2013,31(3):665-677.
[11]杨瑞娟,白建荣,闫蕾,等.玉米低磷胁迫诱导型强启动子P1502-Zm PHR1的克隆与表达分析[J].作物学报,2018,44(7):1000-1009.
[12]王秀红,史向远,白建荣,等.抗草甘膦基因的玉米转化及后代遗传分析[J].华北农学报,2011,26(1):117-121.
[13]王守云,韦正乙,林春晶,等.利用玉米萌动胚转化体系获得抗除草剂转基因新材料[J].玉米科学,2013,21(2):52-57.
[14]孙毅,王景雪,刘少翔,等.农杆菌介导植物萌发种子基因转化方法:中国,CN102212553A[P].2011-10-12.
[15]JEFFERSON R A,KAVANAGH T A,BEVAN M W.GUS fusions:beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants[J].Embo Journal,1987,6(13):3901-3907.
[16]KARABA A,DIXIT S,GRECO R,et al.Improvement of water use efficiency in rice by expression of HARDY,an Arabidopsis drought and salt tolerance gene[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(39):15270-15275.
[17]KASUGA M,LIU Q,MIURA S,et al.Improving plant drought,salt,and freezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nature Biotechnology,1999,17(3):287-291.
[18]RAI M,HE C,WU R.Comparative functional analysis of three abiotic stress-inducible promoters in transgenic rice[J].Transgenic Research,2009,18(5):787-799.
[2]LEE MW,YANG Y.Transient expression vectors for functional genomics,quantification of promoter activity and RNA silencing in plan[J].Methods in Molecular Biology,2006,323:225-229.
[3]KEARNS A,HAWES C,SPARKES I A,et al.Rapid,transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants[J].Nature Protocols,2006,1(4):2019-2025.
[4]邱礽,陶刚,李奇科,等.农杆菌渗入法介导的基因瞬时表达技术及应用[J].分子植物育种,2009(5):1032-1039.
[5]BA譙UELOS MA,GARCIADEBLAS B,CUBERO B,et al.Inventory and functional characterization of the HAK potassium transporters of rice[J].Plant Physiology,2002,130(2):784-795.
[6]刘雪梅,王蕾,文添龙,等.农杆菌介导的棉花子叶瞬时表达系统的建立[J].植物学报,2014(5):587-594.
[7]JENNINGS D H.Mineral nutrition in higher plants(book)[J].Plant Cell&Environment,1988,11(2):147.
[8]RAGHOTHAMA K G,KARTHIKEYAN A S,LAMBERS H,et al.Phosphate acquisition[J].Plant&Soil,2005,274(1/2):37-49.
[9]SMITH F W,MUDGE S R,RAE A L,et al.Phosphate transport in plants[J].Plant&Soil,2003,248(1/2):71-83.
[10]WANG X,BAI J,LIU H,et al.Overexpression of a maize transcription factor Zm PHR1,improves shoot inorganic phosphate content and growth of Arabidopsis,under low-phosphate conditions[J].Plant Molecular Biology Reporter,2013,31(3):665-677.
[11]杨瑞娟,白建荣,闫蕾,等.玉米低磷胁迫诱导型强启动子P1502-Zm PHR1的克隆与表达分析[J].作物学报,2018,44(7):1000-1009.
[12]王秀红,史向远,白建荣,等.抗草甘膦基因的玉米转化及后代遗传分析[J].华北农学报,2011,26(1):117-121.
[13]王守云,韦正乙,林春晶,等.利用玉米萌动胚转化体系获得抗除草剂转基因新材料[J].玉米科学,2013,21(2):52-57.
[14]孙毅,王景雪,刘少翔,等.农杆菌介导植物萌发种子基因转化方法:中国,CN102212553A[P].2011-10-12.
[15]JEFFERSON R A,KAVANAGH T A,BEVAN M W.GUS fusions:beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants[J].Embo Journal,1987,6(13):3901-3907.
[16]KARABA A,DIXIT S,GRECO R,et al.Improvement of water use efficiency in rice by expression of HARDY,an Arabidopsis drought and salt tolerance gene[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(39):15270-15275.
[17]KASUGA M,LIU Q,MIURA S,et al.Improving plant drought,salt,and freezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nature Biotechnology,1999,17(3):287-291.
[18]RAI M,HE C,WU R.Comparative functional analysis of three abiotic stress-inducible promoters in transgenic rice[J].Transgenic Research,2009,18(5):787-799.