青藏高原裸大麦种质资源形态多样性分析
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摘要
为了解青藏高原裸大麦区种质资源的遗传多样性,提高该地区裸大麦种质资源利用效率,对86份青藏裸大麦种质资源的形态多样性进行鉴定。结果表明:该地区裸大麦种质资源遗传多样性丰富,主穗长的形态多样性指数最高,为2.05;株型的遗传多样性指数最低,为0.47。总体表现为数量性状形态多样性指数大于质量性状。基于形态性状的聚类分析把86份材料聚为4类,第Ⅰ类是二棱种质,为高秆大穗大粒型,第Ⅱ类种质为矮秆多粒长穗型,第Ⅲ类种质为短穗型,第Ⅳ类种质为高秆多粒型。采用主成分分析进行种质评价,提取前7个主成分即可概括全部性状的大部分生物学信息,筛选出适合江苏地区生长的优良种质资源,为裸大麦种质资源的收集和利用提供理论依据。
To study the genetic diversity of hulless barley accessions from Qinghai-Tibetan Plateau and improve their utilization efficiency, the morphological diversity of 86 hulless barley accessions from Qinghai-Tibetan Plateau was identified in this study. The results showed that genetic diversity of hulless barley accessions was ample, morphological diversity index of main panicle length was the highest with the value of 2.05. Plant type had the lowest genetic diversity index with the value of 0.47. In general, genetic morphological index of quantitative traits were greater than those of the quality traits. The tested materials were divided into four groups by cluster analysis. Barley accessions in group Ⅰ were two-rowed, high plant height, big spike and big grain. Barley accessions in group Ⅱwere short plant height, multi grain and long spike. Barley accessions in group Ⅲ were short panicle. Barley accessions in group Ⅳ were high plant height and multi grain. Based on the principal components, comprehensive evaluation of various materials was carried out, seven principal components were extracted to summarize most of the biological information of all traits. Excellent germplasm resources suitable for growth in Jiangsu Province were screened out. This study provided a theoretical basis for the collection and utilization of hulless barley accessions.
To study the genetic diversity of hulless barley accessions from Qinghai-Tibetan Plateau and improve their utilization efficiency, the morphological diversity of 86 hulless barley accessions from Qinghai-Tibetan Plateau was identified in this study. The results showed that genetic diversity of hulless barley accessions was ample, morphological diversity index of main panicle length was the highest with the value of 2.05. Plant type had the lowest genetic diversity index with the value of 0.47. In general, genetic morphological index of quantitative traits were greater than those of the quality traits. The tested materials were divided into four groups by cluster analysis. Barley accessions in group Ⅰ were two-rowed, high plant height, big spike and big grain. Barley accessions in group Ⅱwere short plant height, multi grain and long spike. Barley accessions in group Ⅲ were short panicle. Barley accessions in group Ⅳ were high plant height and multi grain. Based on the principal components, comprehensive evaluation of various materials was carried out, seven principal components were extracted to summarize most of the biological information of all traits. Excellent germplasm resources suitable for growth in Jiangsu Province were screened out. This study provided a theoretical basis for the collection and utilization of hulless barley accessions.
引文
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[13] 潘志芬,邹弈星,邓光兵,等.青藏高原栽培青稞SSR标记遗传多样性研究[J].中山大学学报(自然科学版),2007,46(2):82-86.PAN Z F,ZOU Y X,DENG G B,et al.Genetic diversity of SSR markers in cultivated hulless barley from Qinghai-Tibet Plateau in China[J].Acta ScientiarumNaturalium Universitatis Sunyatseni,2007,46(2):82-86.(in Chinese with English abstract)
[14] 吴昆仑.青稞种质资源的SSR标记遗传多样性分析[J].麦类作物学报,2011,31(6):1030-1034.WU K L.Genetic diversity analysis of hulless barley germplasm by SSR markers[J].Journal of Triticeae Crops,2011,31(6):1030-1034.(in Chinese with English abstract)
[15] 原红军,曾兴权,徐其君,等.青稞种质资源遗传多样性分析与核心种质群体的构建[J].麦类作物学报,2018,38(8):922-928.YUAN H J,ZENG X Q,XU Q J,et al.Genetic diversity of germplasm resource and core collection development in hulless barley[J].Journal of Triticeae Crops,2018,38(8):922-928.(in Chinese with English abstract)
[16] 夏腾飞,王蕾,徐金青,等.267份青藏高原青稞种质材料的表型多样性分析[J].西北农业学报,2018,27(2):182-193.XIA T F,WANG L,XU J Q,et al.The genotypic diversity analysis of 267 six-rowed hulless barley accessions from the Qinghai-Tibetan Plateau[J].Acta Agriculturae Boreali-Occidentalis Sinica,2018,27(2):182-193.(in Chinese with English abstract)
[17] 刘亚楠,朱娟,吕超,等.二棱大麦种质的综合评价[J].麦类作物学报,2018,38(4):430-439.LIU Y,ZHU J,LYU C,et al.Comprehensive evaluation of two-rowed barley germplasm[J].Journal of Triticeae Crops,2018,38(4):430-439.(in Chinese with English abstract)
[18] 方彦杰,潘永东,包奇军,等.47份美国大麦种质资源农艺及品质性状分析[J].中国种业,2013(8):63-66.FANG Y J,PAN Y D,BAO Q J,et al.Analysis of agronomic and quality traits of 47 American barley germplasm resources[J].China Seed Industry,2013(8):63-66.(in Chinese)
[19] 尚毅,华为,朱靖环,等.浙江省裸大麦地方品种遗传多样性分析[J].麦类作物学报,2014,34(7):922-928.SHANG Y,HUA W,ZHU J H,et al.Genetic diversity of hulless barley landraces in Zhejiang[J].Journal of Triticeae Crops,2014,34(7):922-928.(in Chinese with English abstract)
[20] 贾巧君,朱靖环,汪军妹,等.浙江赤霉病抗性不同的大麦地方品种遗传多样性分析[J].植物遗传资源学报,2013,14(3):472-478.JIA Q J,ZHU J H,WANG J M,et al.Genetic diversity of Zhejiang barley landraces with different levels of fusarium head blight resistance[J].Journal of Plant Genetic Resources,2013,14(3):472-478.(in Chinese with English abstract)
[21] CHEN Z W,LU R J,ZOU L,et al.Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China[J].Genetics and Molecular Research,2012,11(1):644-650.
[22] 刘遵春,张春雨,张艳敏,等.利用数量性状构建新疆野苹果核心种质的方法[J].中国农业科学,2010,43(2):358-370.LIU Z C,ZHANG C Y,ZHANG Y M,et al.Study on method of constructing core collection of Malus sieversii based on quantitative traits[J].Scientia Agricultura Sinica,2010,43(2):358-370.(in Chinese with English abstract)
[2] 任又成.青海特色作物裸大麦(青稞)的开发及利用[J].青海大学学报(自然科学版),2015,33(4):89-94.REN Y C.Development and utilization of Qinghai featured crop-naked barley[J].Journal of Qinghai University(Natural Science Edition),2015,33(4):89-94.(in Chinese with English abstract)
[3] 张峰,杨勇,赵国华,等.青稞β-葡聚糖研究进展[J].粮食与油脂,2003,16(12):3-5.ZHANG F,YANG Y,ZHAO G H,et al.Advance on beta-glucan from hull-less barley[J].Journal of Cereals & Oils,2003,16(12):3-5.(in Chinese with English abstract)
[4] ZENG X Q,GUO Y,XU Q J,et al.Origin and evolution of qingke barley in Tibet[J].Nature Communications,2018,9:5433.
[5] ZENG X Q,LONG H,WANG Z,et al.The draft genome of Tibetan hulless barley reveals adaptive patterns to the high stressful Tibetan Plateau[J].Proceedings of the National Academy of Sciences,2015,112(4):1095-1100.
[6] 胥婷婷.青稞种质资源遗传多样性分析和核心种质的构建[D].金华:浙江师范大学,2012.XU T T.The genetic diversity and establishment of core collection in hulless barley germplasm resources[D].Jinhua:Zhejiang Normal University,2012.(in Chinese with English abstract)
[7] 董玉琛,曹永生,张学勇,等.中国普通小麦初选核心种质的产生[J].植物遗传资源学报,2003,4(1):1-8.DONG Y S,CAO Y S,ZHANG X Y,et al.Establishment of candidate core collections in Chinese common wheat germplasm[J].Journal of Plant Genetic Resources,2003,4(1):1-8.(in Chinese with English abstract)
[8] 王述民,李立会,黎裕,等.中国粮食和农业植物遗传资源状况报告(Ⅰ)[J].植物遗传资源学报,2011,12(1):1-12.WANG S M,LI L H,LI Y,et al.Status of plant genetic resources for food and agricultural in China(Ⅰ)[J].Journal of Plant Genetic Resources,2011,12(1):1-12.(in Chinese with English abstract)
[9] 王述民,李立会,黎裕,等.中国粮食和农业植物遗传资源状况报告(Ⅱ)[J].植物遗传资源学报,2011,12(2):167-177.WANG S M,LI L H,LI Y,et al.Status of plant genetic resources for food and agricultural in China(Ⅱ)[J].Journal of Plant Genetic Resources,2011,12(2):167-177.(in Chinese with English abstract)
[10] 胡标林,万勇,李霞,等.水稻核心种质表型性状遗传多样性分析及综合评价[J].作物学报,2012,38(5):829-839.HU B L,WAN Y,LI X,et al.Analysis on genetic diversity of phenotypic traits in rice (Oryza sativa) core collection and its comprehensive assessment[J].Acta Agronomica Sinica,2012,38(5):829-839.(in Chinese with English abstract)
[11] 代攀虹,孙君灵,何守朴,等.陆地棉核心种质表型性状遗传多样性分析及综合评价[J].中国农业科学,2016,49(19):3694-3708.DAI P H,SUN J L,HE S P,et al.Comprehensive evaluation and genetic diversity analysis of phenotypic traits of core collection in upland cotton[J].Scientia Agricultura Sinica,2016,49(19):3694-3708.(in Chinese with English abstract)
[12] 王海岗,贾冠清,智慧,等.谷子核心种质表型遗传多样性分析及综合评价[J].作物学报,2016,42(1):19-30.WANG H G,JIA G Q,ZHI H,et al.Phenotypic diversity evaluations of foxtail millet core collections[J].Acta Agronomica Sinica,2016,42(1):19-30.(in Chinese with English abstract)
[13] 潘志芬,邹弈星,邓光兵,等.青藏高原栽培青稞SSR标记遗传多样性研究[J].中山大学学报(自然科学版),2007,46(2):82-86.PAN Z F,ZOU Y X,DENG G B,et al.Genetic diversity of SSR markers in cultivated hulless barley from Qinghai-Tibet Plateau in China[J].Acta ScientiarumNaturalium Universitatis Sunyatseni,2007,46(2):82-86.(in Chinese with English abstract)
[14] 吴昆仑.青稞种质资源的SSR标记遗传多样性分析[J].麦类作物学报,2011,31(6):1030-1034.WU K L.Genetic diversity analysis of hulless barley germplasm by SSR markers[J].Journal of Triticeae Crops,2011,31(6):1030-1034.(in Chinese with English abstract)
[15] 原红军,曾兴权,徐其君,等.青稞种质资源遗传多样性分析与核心种质群体的构建[J].麦类作物学报,2018,38(8):922-928.YUAN H J,ZENG X Q,XU Q J,et al.Genetic diversity of germplasm resource and core collection development in hulless barley[J].Journal of Triticeae Crops,2018,38(8):922-928.(in Chinese with English abstract)
[16] 夏腾飞,王蕾,徐金青,等.267份青藏高原青稞种质材料的表型多样性分析[J].西北农业学报,2018,27(2):182-193.XIA T F,WANG L,XU J Q,et al.The genotypic diversity analysis of 267 six-rowed hulless barley accessions from the Qinghai-Tibetan Plateau[J].Acta Agriculturae Boreali-Occidentalis Sinica,2018,27(2):182-193.(in Chinese with English abstract)
[17] 刘亚楠,朱娟,吕超,等.二棱大麦种质的综合评价[J].麦类作物学报,2018,38(4):430-439.LIU Y,ZHU J,LYU C,et al.Comprehensive evaluation of two-rowed barley germplasm[J].Journal of Triticeae Crops,2018,38(4):430-439.(in Chinese with English abstract)
[18] 方彦杰,潘永东,包奇军,等.47份美国大麦种质资源农艺及品质性状分析[J].中国种业,2013(8):63-66.FANG Y J,PAN Y D,BAO Q J,et al.Analysis of agronomic and quality traits of 47 American barley germplasm resources[J].China Seed Industry,2013(8):63-66.(in Chinese)
[19] 尚毅,华为,朱靖环,等.浙江省裸大麦地方品种遗传多样性分析[J].麦类作物学报,2014,34(7):922-928.SHANG Y,HUA W,ZHU J H,et al.Genetic diversity of hulless barley landraces in Zhejiang[J].Journal of Triticeae Crops,2014,34(7):922-928.(in Chinese with English abstract)
[20] 贾巧君,朱靖环,汪军妹,等.浙江赤霉病抗性不同的大麦地方品种遗传多样性分析[J].植物遗传资源学报,2013,14(3):472-478.JIA Q J,ZHU J H,WANG J M,et al.Genetic diversity of Zhejiang barley landraces with different levels of fusarium head blight resistance[J].Journal of Plant Genetic Resources,2013,14(3):472-478.(in Chinese with English abstract)
[21] CHEN Z W,LU R J,ZOU L,et al.Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China[J].Genetics and Molecular Research,2012,11(1):644-650.
[22] 刘遵春,张春雨,张艳敏,等.利用数量性状构建新疆野苹果核心种质的方法[J].中国农业科学,2010,43(2):358-370.LIU Z C,ZHANG C Y,ZHANG Y M,et al.Study on method of constructing core collection of Malus sieversii based on quantitative traits[J].Scientia Agricultura Sinica,2010,43(2):358-370.(in Chinese with English abstract)