生姜组培快繁技术研究
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摘要
为解决海南生姜产量低、抗病能力弱等问题,本研究以四川乐山白种生姜为试材,采用不同激素配比随机区组试验,对生姜组培快繁技术进行研究。结果表明:(1)6-BA对姜芽分化最为有效,培养基为MS+6-BA2.0 mg/L+NAA 0.1 mg/L最有利于芽的分化;(2)在培养基MS+6-BA (1.0～4.0 mg/L)+NAA(0.1～0.5 mg/L)上,可以实现生姜增殖与生根诱导同步进行,培养30 d后,繁殖系数5.0以上,生根诱导率达100%;(3)沙、土混体积比6∶4作育苗基质的移栽成活率最高达100%。本研究筛选出适合生姜组培的培养基组合及育苗基质,为生姜在海南大面积种植提供理论依据。
In order to solve th e problems of low yield and low disease resistance of ginger in Hainan,ginger variety Sichuan Leshan White was tissue cultured for rapid propagation in a randomized block experiment with different combinations of hormones.The results showed that 6-BA was most effective in ginger differentiation and that the medium MS +6-BA 2.0 mg/L + NAA 0.1 mg/L had the best effect on the differentiation of ginger shoot.The shoot proliferation and root induction were synchronized in the culture medium MS + 6-BA(1.0～4.0 mg/L) + NAA(0.1～0.5 mg/L).The proliferation rate was higher than 5.0,and the rooting induction rate was 100% after 30 days of culture.The transplanting survival rate of the tissue cultured plants cultured in the nursery substrate(sand∶soil=6∶4) was up to 100%.The medium combination and the substrate selected for ginger tissue culture will provide possible theoretical basis for ginger planting in Hainan.
In order to solve th e problems of low yield and low disease resistance of ginger in Hainan,ginger variety Sichuan Leshan White was tissue cultured for rapid propagation in a randomized block experiment with different combinations of hormones.The results showed that 6-BA was most effective in ginger differentiation and that the medium MS +6-BA 2.0 mg/L + NAA 0.1 mg/L had the best effect on the differentiation of ginger shoot.The shoot proliferation and root induction were synchronized in the culture medium MS + 6-BA(1.0～4.0 mg/L) + NAA(0.1～0.5 mg/L).The proliferation rate was higher than 5.0,and the rooting induction rate was 100% after 30 days of culture.The transplanting survival rate of the tissue cultured plants cultured in the nursery substrate(sand∶soil=6∶4) was up to 100%.The medium combination and the substrate selected for ginger tissue culture will provide possible theoretical basis for ginger planting in Hainan.
引文
[1]尚小红,周生茂,文俊丽,等.生姜组织培养的影响因子[J].北方园艺, 2012(1):173-176.
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[3]孙晓杨.姜青枯腐败病发生规律和系统控制技术[J].云南农业大报, 1998, 13(1):122-125.
[4]罗怀海.我国姜瘟研究现状[J].植物保护, 1995, 21(1):38-40.
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[7]高山林,卞云云,陈柏君.生姜组织培养脱病毒、快繁和高产栽培[J].中国蔬菜, 1999(3):40-41.
[8]徐美玲,葛胜娟,费伟英,等.生姜快繁及移栽技术[J].浙江农业科学, 2005(6):444-445.
[9]葛胜娟.生姜组培苗的培育及其生产应用[J].中国农学通报, 2007, 23(5):75-78.
[10]静一,雷开荣,陈旭,等.生姜组培快繁技术优化研究[J].南方农业, 2009(5):62-64.
[11]杭玲,黄卓忠,等.生姜组织培养快繁技术研究与应用[J].江苏农业科学, 2006(5):125-127.
[12]黄皓,周生茂,尚小红,等.西林火姜健康组培苗快繁体系的建立[J].长江蔬菜, 2016(20):25-29.
[13]吴家丽,赵佐敏,等.白水生姜组培脱毒及离体快繁体系建立研究[J].长江蔬菜, 2011(4):17-20.
[2]葛胜娟,平培元,徐美玲,等.不同苗质及移栽条件对新丰生姜组培苗成活率的影响[J].中国农学通报,2003(4):54-56.
[3]孙晓杨.姜青枯腐败病发生规律和系统控制技术[J].云南农业大报, 1998, 13(1):122-125.
[4]罗怀海.我国姜瘟研究现状[J].植物保护, 1995, 21(1):38-40.
[5]林永寿.姜青枯腐败病的防治研究[J].四川农业科技, 1988(2):14-15.
[6]齐兰,朱红林,陈健晓,等.海南小黄姜脱毒快繁技术研究[J].热带农业科学, 2013, 33(9):37-40.
[7]高山林,卞云云,陈柏君.生姜组织培养脱病毒、快繁和高产栽培[J].中国蔬菜, 1999(3):40-41.
[8]徐美玲,葛胜娟,费伟英,等.生姜快繁及移栽技术[J].浙江农业科学, 2005(6):444-445.
[9]葛胜娟.生姜组培苗的培育及其生产应用[J].中国农学通报, 2007, 23(5):75-78.
[10]静一,雷开荣,陈旭,等.生姜组培快繁技术优化研究[J].南方农业, 2009(5):62-64.
[11]杭玲,黄卓忠,等.生姜组织培养快繁技术研究与应用[J].江苏农业科学, 2006(5):125-127.
[12]黄皓,周生茂,尚小红,等.西林火姜健康组培苗快繁体系的建立[J].长江蔬菜, 2016(20):25-29.
[13]吴家丽,赵佐敏,等.白水生姜组培脱毒及离体快繁体系建立研究[J].长江蔬菜, 2011(4):17-20.