摘要
为解决海南生姜产量低、抗病能力弱等问题,本研究以四川乐山白种生姜为试材,采用不同激素配比随机区组试验,对生姜组培快繁技术进行研究。结果表明:(1)6-BA对姜芽分化最为有效,培养基为MS+6-BA2.0 mg/L+NAA 0.1 mg/L最有利于芽的分化;(2)在培养基MS+6-BA (1.0~4.0 mg/L)+NAA(0.1~0.5 mg/L)上,可以实现生姜增殖与生根诱导同步进行,培养30 d后,繁殖系数5.0以上,生根诱导率达100%;(3)沙、土混体积比6∶4作育苗基质的移栽成活率最高达100%。本研究筛选出适合生姜组培的培养基组合及育苗基质,为生姜在海南大面积种植提供理论依据。
In order to solve th e problems of low yield and low disease resistance of ginger in Hainan,ginger variety Sichuan Leshan White was tissue cultured for rapid propagation in a randomized block experiment with different combinations of hormones.The results showed that 6-BA was most effective in ginger differentiation and that the medium MS +6-BA 2.0 mg/L + NAA 0.1 mg/L had the best effect on the differentiation of ginger shoot.The shoot proliferation and root induction were synchronized in the culture medium MS + 6-BA(1.0~4.0 mg/L) + NAA(0.1~0.5 mg/L).The proliferation rate was higher than 5.0,and the rooting induction rate was 100% after 30 days of culture.The transplanting survival rate of the tissue cultured plants cultured in the nursery substrate(sand∶soil=6∶4) was up to 100%.The medium combination and the substrate selected for ginger tissue culture will provide possible theoretical basis for ginger planting in Hainan.
引文
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