双参口服液含量测定方法的研究
|
摘要
目的:建立双参口服液中丹参酮Ⅱ_A的含量测定方法。方法:流动相:甲醇-水(75∶25);检测波长:268nm;柱度:30℃;流速:1m L/min。结果:丹参酮Ⅱ_A在0.081~0.504mg/m L线性关系良好(R=0.9997);平均回收率为98.25%,RSD为3.39%(n=6)。结论:本研究建立的HPLC法专属性强、准确度高、重复性好,可用于双参口服液中丹参酮ⅡA的含量测定。
Objective:To determine The Content Of tanshinone Ⅱ_A In Shuangshen Oral liquid By HPLC. Methods:mobile phase:methanol-water(75 ∶25),detection wavelength:268 nm,column temperature :30℃,flow rate: 1.0 m L/min. Results:tanshinone Ⅱ_A was detected in the range from 20 ~100μg0.081 ~0.504 mg/m L indicating good linear relationship,R2=0.9997,the mean sample recovery rate was 98.25% and the RSD was 3.39%(n =6). Conclusion:The HPLC method is accurate in operation,high precision and good in reproducibility,and can be taken as the determination of tanshinone Ⅱ_A In Shuangshen Oral liquid.
Objective:To determine The Content Of tanshinone Ⅱ_A In Shuangshen Oral liquid By HPLC. Methods:mobile phase:methanol-water(75 ∶25),detection wavelength:268 nm,column temperature :30℃,flow rate: 1.0 m L/min. Results:tanshinone Ⅱ_A was detected in the range from 20 ~100μg0.081 ~0.504 mg/m L indicating good linear relationship,R2=0.9997,the mean sample recovery rate was 98.25% and the RSD was 3.39%(n =6). Conclusion:The HPLC method is accurate in operation,high precision and good in reproducibility,and can be taken as the determination of tanshinone Ⅱ_A In Shuangshen Oral liquid.
引文
[1]国家药典委员会.中华人民共和国药典2010年版[S].北京化学工业出版社,2010.
[2]杨武军,刘雪峰.反相高效液相色谱法测定筋骨痛消丸中丹参酮ⅡA的含量[J].现代医药卫生,2009,25(12):1787.
[3]王朝宇,白秀云,白海玉,等.丹芪祛瘀止痛颗粒质量标准的研究[J].中国实验方剂学杂志,2011,17(4):76-79.
[2]杨武军,刘雪峰.反相高效液相色谱法测定筋骨痛消丸中丹参酮ⅡA的含量[J].现代医药卫生,2009,25(12):1787.
[3]王朝宇,白秀云,白海玉,等.丹芪祛瘀止痛颗粒质量标准的研究[J].中国实验方剂学杂志,2011,17(4):76-79.