摘要
目的对结核肺组织与正常肺组织双向电泳图谱进行比较分析,筛选差异表达蛋白质。方法利用双向电泳分离结核组及正常组总蛋白质,并通过计算机图像分析软件分析电泳图谱。结果在结核肺组织中检测有效蛋白点数为(190±11)个,正常肺组织中检测有效蛋白点数为(179±5)个。发现两者间共有15个差异蛋白点,蛋白点分子量(Mr)主要分布于15kD~80kD,PI为3~10。4个点在病灶组织中表达上调,11个点在病灶组织中表达下调。结论通过双向凝胶电泳和图像分析技术对结核肺组织及正常肺组织蛋白质组进行研究,获得了结核组与正常组的差异蛋白点,为进一步利用质谱分析技术做差异蛋白质鉴定奠定了基础,并为肺结核的早期诊断、发病机制探索、病情监测提供依据。
Objective To analyze the two dimentional electrophoresis( 2-DE) maps of lung tissue from pulmonary tuberculosis and normal lesions and screen differently expressed proteins between them. Methods 2-DE( two dimentional electrophoresis) was used to sever the general protein from lung tissues in pulmonary tuberculosis and normal lesions,then the results were analyzed by computer-graph analysis software. Results 190 ± 11 protein spots were verified in pulmonary tuberculosis tissue and 179 ± 5 ones in normal lung tissue. There were 15 discrepancy protein spots. The protein point molecular weight( Mr) is mainly distributed in 15 kD ~ 80 kD,isoelectric point( PI) is 3 ~ 10. Meanwhile,4 spots frequently emerged and 11 ones were less expressed in pulmonary tuberculosis tissue. Conclusion The discrepancy protein spots were identified,which laid a solid foundation for further research of discrepancy protein,and provide basis for early diagnosis,pathogenesis and monitoring of pulmonary tuberculosis.
引文
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