敲除FGF5基因对陕北白绒山羊血液生理生化指标的影响
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摘要
为探究敲除FGF5基因对陕北白绒山羊血液生理生化指标的影响,本实验以敲除FGF5基因的陕北白绒山羊(公、母羊各8只,阳性)、同龄普通陕北白绒山羊(公、母羊各5只,阴性)为研究对象,分别在10月龄、1岁与1.5岁时测定8项血常规指标和14项血液生化指标。结果表明:血常规指标中,10月龄、1岁时,阳性公羊的红细胞压积(HCT)、平均红细胞血红蛋白含量(MCH)显著低于阴性公羊(P<0.05),阳性母羊的红细胞分布宽度变异系数(RDW)、血红蛋白(HGB)、HCT极显著高于阴性母羊(P<0.01);1岁时,阳性公羊和母羊的红细胞数目(RBC)均显著高于阴性公羊和母羊(P<0.05);1.5岁时,阳性母羊的白细胞数目(WBC)显著高于阴性母羊(P<0.05)。血液生化指标中,10月龄时,阳性母羊的球蛋白极显著低于阴性母羊(P<0.01);1岁、1.5岁时,阳性母羊的白蛋白极显著高于阴性母羊(P<0.01);1.5岁时,阳性母羊的尿素极显著低于阴性母羊(P<0.01);其余指标均差异不显著(P>0.05)。因所有指标均在参考范围内,说明敲除FGF5基因的陕北白绒山羊的蛋白质代谢、糖代谢、脂类代谢、肝胆功能、钙磷代谢均无显著影响。表明采用CRISPR/Cas9技术敲除FGF5基因并未对陕北白绒山羊的健康产生影响,但部分存在显著差异的血液生理生化指标有待进一步研究。
The experiment was conducted to study the effect of FGF5(Fibroblast Growth Factor-5) gene knockout on the physiological and bio-chemical parameters of the blood of Shanbei cashmere goats. The experimental animals were 8 FGF5-knockout female Shanbei cashmere goats(knockout female group), 8 FGF5-knockout male Shanbei White cashmere goats(knockout male group), 5 female Shanbei White cashmere goats(control female group) and 5 male Shanbei cashmere goats(control male group).The levels of WBC, RBC, HGB, HCT, MCV, MCH, MCHC and RDW in the blood of Shanbei White cashmere goats at the age of 10, 12 or 18 month old were determined, meanwhile the activities of AMY, ALT, ALP and CK, the levels of TP, ALB, GLO, Ca, GLU, Urea, P, TC, TBIL and CREA in the blood were measured too.Results: The results showed that the blood routine parameters were not significant(P>0.05) except for RDW(P<0.05) of the knockout and the control groups, while all the parameters were within the normal range. The blood routine parameters differed within the normal value among Shanbei cashmere goats of different ages and genders. The blood biochemical indexes were different of Shanbei cashmere goats of different ages and genders but they were within the normal range. The protein metabolism, glucose and lipid metabolism, liver and gallbladder function, metabolism of calcium and phosphorus of the knockout FGF5 Shanbei White cashmere goats had no significant difference.Conclusion: The blood biochemical indexes were different of Shanbei white cashmere goats of different ages and genders but they were within the normal range. There were no significant difference between the knockout group and the control group. The results demonstrated that CRISPR/Cas9-edited FGF5 gene had no significant difference on the blood biochemical indexes of Shanbei cashmere goats.
The experiment was conducted to study the effect of FGF5(Fibroblast Growth Factor-5) gene knockout on the physiological and bio-chemical parameters of the blood of Shanbei cashmere goats. The experimental animals were 8 FGF5-knockout female Shanbei cashmere goats(knockout female group), 8 FGF5-knockout male Shanbei White cashmere goats(knockout male group), 5 female Shanbei White cashmere goats(control female group) and 5 male Shanbei cashmere goats(control male group).The levels of WBC, RBC, HGB, HCT, MCV, MCH, MCHC and RDW in the blood of Shanbei White cashmere goats at the age of 10, 12 or 18 month old were determined, meanwhile the activities of AMY, ALT, ALP and CK, the levels of TP, ALB, GLO, Ca, GLU, Urea, P, TC, TBIL and CREA in the blood were measured too.Results: The results showed that the blood routine parameters were not significant(P>0.05) except for RDW(P<0.05) of the knockout and the control groups, while all the parameters were within the normal range. The blood routine parameters differed within the normal value among Shanbei cashmere goats of different ages and genders. The blood biochemical indexes were different of Shanbei cashmere goats of different ages and genders but they were within the normal range. The protein metabolism, glucose and lipid metabolism, liver and gallbladder function, metabolism of calcium and phosphorus of the knockout FGF5 Shanbei White cashmere goats had no significant difference.Conclusion: The blood biochemical indexes were different of Shanbei white cashmere goats of different ages and genders but they were within the normal range. There were no significant difference between the knockout group and the control group. The results demonstrated that CRISPR/Cas9-edited FGF5 gene had no significant difference on the blood biochemical indexes of Shanbei cashmere goats.
引文
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[10]朱蓉慧,陈玉林,屈雷,等.陕北白绒山羊10月龄母羊发情周期血清激素分泌规律的研究[J].中国畜牧杂志,2017,53(9):61-65.
[11]乔娜,干驰,朱佳琪,等.转hLF基因山羊血液生理生化指标检测分析[J].南京农业大学学报,2016,5(3):483-487.
[12]郭辉.山羊在不同日粮结构下尿中嘌呤衍生物排出规律的研究[D].南宁:广西大学,2008.
[13]焦永成,王玉琴,达赖,等.引入河南省杜泊羊血液生理生化指标测定分析[J].黑龙江畜牧兽医,2016,(19):91-94.
[14]左之才,邓俊良,王哲,等.不同能量摄入水平对围产期健康奶牛血清总胆红素、蛋白及转氨酶的影响[J].中国兽医学报,2007,27(6):865-869.
[15]张文丽.会理黑山羊血液生化指标的测定[J].黑龙江畜牧兽医,2014(14):40-42.
[16]吴荣华,封定国,施云冈,等.高原应激对山羊血液一些生理生化指标的影响[J].动物医学进展,2014,35(2):63-65.
[2]沈彦花,赵永聚.CRISPR/Cas9技术及其在绵、山羊遗传研究上的应用潜力[A].全国养羊生产与学术研讨会论文集[C].郑州:中国畜牧兽医学会羊羊学分会,2015.
[3]何晓琳.陕北白绒山羊FGF5基因及其转基因羊的研究[D].杨凌:西北农林科技大学,2015.
[4]呼锐.利用CRISPR/Cas9技术一步生产FGF5基因敲除绵羊的研究[D].北京:中国农业大学,2017.
[5]陈勇,王泰,苏元君,等.不同年龄的贾洛羊生理生化指标测定[J].西南民族大学学报:自然科学版,2015,41(6):667-671.
[6]闫昱,屈雷.陕北白绒山羊产业发展现状和策略研究[J].榆林学院学报,2008,18(2):10-15
[7]王小龙,朱海鲸,周建奎,等.通过CRISPR/Cas9介导的基因打靶技术获得基因修饰的绒山羊[A].遗传多样性:前言与挑战——中国的遗传学研究(2013~2015)——2015中国遗传学会大会论文摘要汇编[C].昆明:中国遗传学会,2015.
[8]刘萍,白云峰,刘建,等.大豆皮替代饲粮中稻草对波杂山羊生长性能、营养成分消化率和血液生化指标的影响[J].中国畜牧杂志,2017,53(10):84-88.
[9]罗洪明,陈代文.不同蛋白水平对早期断奶仔猪生产性能、血液生化指标的影响[J].饲料研究,2008(8):3-8.
[10]朱蓉慧,陈玉林,屈雷,等.陕北白绒山羊10月龄母羊发情周期血清激素分泌规律的研究[J].中国畜牧杂志,2017,53(9):61-65.
[11]乔娜,干驰,朱佳琪,等.转hLF基因山羊血液生理生化指标检测分析[J].南京农业大学学报,2016,5(3):483-487.
[12]郭辉.山羊在不同日粮结构下尿中嘌呤衍生物排出规律的研究[D].南宁:广西大学,2008.
[13]焦永成,王玉琴,达赖,等.引入河南省杜泊羊血液生理生化指标测定分析[J].黑龙江畜牧兽医,2016,(19):91-94.
[14]左之才,邓俊良,王哲,等.不同能量摄入水平对围产期健康奶牛血清总胆红素、蛋白及转氨酶的影响[J].中国兽医学报,2007,27(6):865-869.
[15]张文丽.会理黑山羊血液生化指标的测定[J].黑龙江畜牧兽医,2014(14):40-42.
[16]吴荣华,封定国,施云冈,等.高原应激对山羊血液一些生理生化指标的影响[J].动物医学进展,2014,35(2):63-65.