不同基因型燕麦苗期耐盐碱性分析及其鉴定指标的筛选
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摘要
为探讨不同基因型燕麦苗期的耐盐碱性,筛选适宜松嫩平原种植的燕麦品种,本研究采用Hoagland营养液水培法,以250 mmol·L~(-1)高浓度NaHCO_3胁迫模拟松嫩平原盐碱环境,对49份来自国内外不同地区的主栽燕麦品种进行研究。试验测定了250 mmol·L~(-1) NaHCO_3胁迫下燕麦的株高(X_1)、根长(X_2)、地上部鲜重(X_3)、地下部鲜重(X_4)、地上部干重(X_5)、地下部干重(X_6)、地上部含水量(X_7)、地下部含水量(X_8)、根冠比(X_9)9个指标,以各单项指标的耐盐碱系数(SATC)作为衡量耐盐碱性的依据,利用多元分析方法对不同燕麦品种耐盐碱能力进行了综合评价。结果表明:通过对各指标的耐盐碱系数进行主成分分析,得到X_4、X_7、X_8 3个综合指标,涵盖了全部数据86.155%的信息量;通过隶属函数分析和聚类分析将49份燕麦品种分为3类,其中草莜1号、张燕7、T7等3个品种为耐盐碱品种,HLJ 1、白燕2号等41个品种为中度耐盐碱品种,三分三、坝莜13号等5个品种为盐碱敏感品种。
In order to explore the salt alkaline bearing capacity of different oat cultivars in seedling stage and screen the oat cultivars, which are well suited for Songnen Plain to grow. This research used Hoagland hydroponics with high concentrations of 250 mmol·L~(-1) NaHCO_3 stress to simulate the saline conditions of Songnen Plain, and carried out a study on 49 major oat cultivars, which came from China and abroad. Nine indexes including plant height(X_1), root length(X_2), aboveground fresh weight(X_3), underground fresh weight(X_4), aboveground dry weight(X_5), underground dry weight(X_6), aboveground water content(X_7), underground water content(X_8), and root shoot ratio(X_9) were measured under 250 mmol·L~(-1) NaHCO_3 stress. Based on the saline-alkaline tolerance coefficient(SATC) of each individual indicator, different oat cultivars were evaluated by principal components analysis, subordinate function analysis, and clustering analysis. The results showed that three comprehensive indexes including X_4, X_7 and X_8 were obtained by principle component analysis of saline-alkaline tolerance coefficients of all indexes. The comprehensive indexes described 86.155% information of all data. The 49 oat cultivars were divided into three groups through subordinate function analysis and clustering analysis. Among them, Caoyou 1, Zhangyan 7 and T7 were salt-tolerant cultivars, 41 cultivars such as HLJ 1, Baiyan 2 were moderate tolerant to saline-alkaline, 5 cultivars such as Sanfensan, Bayou 13 were sensitive to saline-alkaline tolerance.
In order to explore the salt alkaline bearing capacity of different oat cultivars in seedling stage and screen the oat cultivars, which are well suited for Songnen Plain to grow. This research used Hoagland hydroponics with high concentrations of 250 mmol·L~(-1) NaHCO_3 stress to simulate the saline conditions of Songnen Plain, and carried out a study on 49 major oat cultivars, which came from China and abroad. Nine indexes including plant height(X_1), root length(X_2), aboveground fresh weight(X_3), underground fresh weight(X_4), aboveground dry weight(X_5), underground dry weight(X_6), aboveground water content(X_7), underground water content(X_8), and root shoot ratio(X_9) were measured under 250 mmol·L~(-1) NaHCO_3 stress. Based on the saline-alkaline tolerance coefficient(SATC) of each individual indicator, different oat cultivars were evaluated by principal components analysis, subordinate function analysis, and clustering analysis. The results showed that three comprehensive indexes including X_4, X_7 and X_8 were obtained by principle component analysis of saline-alkaline tolerance coefficients of all indexes. The comprehensive indexes described 86.155% information of all data. The 49 oat cultivars were divided into three groups through subordinate function analysis and clustering analysis. Among them, Caoyou 1, Zhangyan 7 and T7 were salt-tolerant cultivars, 41 cultivars such as HLJ 1, Baiyan 2 were moderate tolerant to saline-alkaline, 5 cultivars such as Sanfensan, Bayou 13 were sensitive to saline-alkaline tolerance.
引文
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[16] 高建明,夏卜贤,袁庆华,等.高粱种质材料幼苗期耐盐碱性评价[J].应用生态学报,2012,23(5):1303-1310.
[17] 马晓军,金峰学,杨姗,等.作物耐盐碱数量性状基因座(QTL)定位[J].分子植物育种,2015,13(1):221-227.
[18] 武俊英.燕麦耐盐生理特性及农艺措施调控研究[D].呼和浩特:内蒙古农业大学,2009:34-35.
[19] 王宝山,赵可夫,邹琦.作物耐盐机理研究进展及提高作物抗盐性的对策[J].植物学通报,1997,14(Z1):26-31.
[20] 刘妍妍,吴纪中,许璋阳,等.人工海水胁迫下小麦芽期和苗期的耐盐性鉴定方法[J].植物生理学报,2014,50(2):214-222.
[21] Koyama M L,Levesley A,Koebner R M.Quantitative trait loci for component physiological traits determining salt tolerance in rice [J].Plant Physiology,2001,125(1):406-422.
[22] Falster D S,Westoby M.Plant height and evolutionary games [J].Trends in Ecology and Evolution,2003,18(7):337-343.
[23] 张翯,顾万荣,王泳超,等.DCPTA对盐胁迫下玉米苗期根系生长、渗透调节及膜透性的影响[J].生态学杂志,2015,34(9):2474-2481.
[24] 白健慧.燕麦对盐碱胁迫的生理响应机制研究[D].呼和浩特:内蒙古农业大学,2016.
[25] Meloni D A,Oliva M A,Ruiz H A,et al.Contribution of proline and inorganic solutes to osmotic adjustment in cotton under salt stress [J].Journal of Plant Nutrition,2001,24(3):599-612.
[26] Winicov I.New molecular approaches to improving salt tolerance in crop plants [J].Annals of Botany,1998,82(6):703 -710.
[27] 李擎天,赵宇,周绮云,等.珠眉海棠水通道蛋白MzPIP1;1基因的克隆与表达分析[J].中国农业大学学报,2012,17(3):63-68.
[28] 张国伟,路海玲,张雷,等.棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J].应用生态学报,2011,22(8):2045-2053.
[2] Boyer J S.Plant productivity and environment[J].Science,1982,218(4571):443-448.
[3] Zhu J K.Plant salt tolerance [J].Trends in plant science,2001,6(2):66-71.
[4] 李文昊,王振华,郑旭荣,等.新疆绿洲盐碱地滴灌条件下地下水局部动态对荒地盐分的影响[J].干旱区研究,2016,33(5):1110-1118.
[5] 冯玉杰,张巍,陈桥,等.松嫩平原盐碱化草原土壤理化特性及微生物结构分析[J].土壤,2007,39(2):301-305.
[6] Wang L X,Fang C,Wang K.Physiological responses of Leymus Chinensis to long-term salt,alkali and mixed salt-alkali stresses [J].Journal of Plant Nutrition,2015,38(4):526-540.
[7] 张婷婷,于崧,于立河,等.松嫩平原春小麦耐盐碱性鉴定及品种(系)筛选[J].麦类作物学报,2016,36(8):1008-1019.
[8] 李琳,于崧,蒋永超,等.芸豆苗期耐盐碱性鉴定及品种筛选研究[J].植物生理学报,2016,52(1):62-72.
[9] 张春宵,袁英,刘文国,等.玉米杂交种苗期耐盐碱筛选与大田鉴定的比较分析[J].玉米科学,2010,18(5):14-18.
[10] 马艳明,刘敏,冯魁,等.新疆冬小麦选育品种苗期耐盐性鉴定[J].新疆农业科学,2017,54(8):1394-1401.
[11] 刘雅辉,王秀萍,张国新,等.棉花苗期耐盐形态指标的筛选及综合评价[J].安徽农业科学,2012,40(9):5119-5120,5721.
[12] 杨科,张保军,胡银岗,等.混合盐碱胁迫对燕麦种子萌发及幼苗生理生化特性的影响[J].干旱地区农业研究,2009,27(3):188-192.
[13] 范远,任长忠,李品芳,等.盐碱胁迫下燕麦生长及阳离子吸收特征[J].应用生态学报,2011,22(11):2875-2882.
[14] 李英丽,方正,毛明艳.不同燕麦品种耐碱性筛选和鉴定[J].河北农业大学学报,2014,37(6):13-17.
[15] 葛军勇,臧华栋,钱欣,等.白城地区皮燕麦肥料效应与推荐施肥研究[J].作物杂志,2015,(3):98-103.
[16] 高建明,夏卜贤,袁庆华,等.高粱种质材料幼苗期耐盐碱性评价[J].应用生态学报,2012,23(5):1303-1310.
[17] 马晓军,金峰学,杨姗,等.作物耐盐碱数量性状基因座(QTL)定位[J].分子植物育种,2015,13(1):221-227.
[18] 武俊英.燕麦耐盐生理特性及农艺措施调控研究[D].呼和浩特:内蒙古农业大学,2009:34-35.
[19] 王宝山,赵可夫,邹琦.作物耐盐机理研究进展及提高作物抗盐性的对策[J].植物学通报,1997,14(Z1):26-31.
[20] 刘妍妍,吴纪中,许璋阳,等.人工海水胁迫下小麦芽期和苗期的耐盐性鉴定方法[J].植物生理学报,2014,50(2):214-222.
[21] Koyama M L,Levesley A,Koebner R M.Quantitative trait loci for component physiological traits determining salt tolerance in rice [J].Plant Physiology,2001,125(1):406-422.
[22] Falster D S,Westoby M.Plant height and evolutionary games [J].Trends in Ecology and Evolution,2003,18(7):337-343.
[23] 张翯,顾万荣,王泳超,等.DCPTA对盐胁迫下玉米苗期根系生长、渗透调节及膜透性的影响[J].生态学杂志,2015,34(9):2474-2481.
[24] 白健慧.燕麦对盐碱胁迫的生理响应机制研究[D].呼和浩特:内蒙古农业大学,2016.
[25] Meloni D A,Oliva M A,Ruiz H A,et al.Contribution of proline and inorganic solutes to osmotic adjustment in cotton under salt stress [J].Journal of Plant Nutrition,2001,24(3):599-612.
[26] Winicov I.New molecular approaches to improving salt tolerance in crop plants [J].Annals of Botany,1998,82(6):703 -710.
[27] 李擎天,赵宇,周绮云,等.珠眉海棠水通道蛋白MzPIP1;1基因的克隆与表达分析[J].中国农业大学学报,2012,17(3):63-68.
[28] 张国伟,路海玲,张雷,等.棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J].应用生态学报,2011,22(8):2045-2053.