摘要
以新疆南山、库尔德宁等地区感染白腐病的雪岭云杉为材料,从中分离得到4株具有木质素降解能力的真菌,并确定其分类学地位,初步研究菌株对木质素的降解能力。通过含有0.04%愈创木酚的马铃薯培养基进行初筛,含有1%的苯胺蓝的马铃薯培养基进行复筛,扫描电镜观察法及称重法对木质素降解能力进行初步测定,得到2-A、1-G、2-B、3-E四种菌,对木质素降解率分别为5.82%、6.23%、3.80%、5.14%。18S r DNA序列分析确定2-A在分类学上属于葡萄座腔菌目,葡萄座腔菌科,微壳色单隔孢属(Microdiplodia sp.); 1-G为三隔镰孢菌(Fusarium tricinctum),镰孢菌属(Fusarium)。
Collecting rotting wood of spruce in Xinjiang,such as Nam Shan,Aletai Kuerdening,for research meteaial. Lignin degrading fungi from infected spruce were extracted and separated,its taxonomic status were determined,and the lignin degradation rate were tested. Using potato medium containing 0.04% guaiacol for screening,containing 1% aniline blue for rescreening,then four lignin degrading ability of the fungi were dicovered by electronic microscopy and weighing method. The resulting lignin degradation rate of 2-A,1-G,2-B,3-E were 5. 82%,6. 23%,3. 80%,5. 14%,respectively. Using the measured 18 S r DNA sequence characterization,2-A in the taxonomy belongs to Botryosphaeriales,Botryosphaeriaceae,Microdiplodia sp.; 1-G belongs to the Fusarium sp.
引文
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