老虎须组培实生苗快繁技术及其生根炼苗的移栽效果
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摘要
为快速繁殖老虎须实生苗应用于实际,采用老虎须种子进行消毒,接种获得无菌实生苗,用叶片和叶柄诱导愈伤组织,进行增殖培养、生根培养和炼苗移栽。结果表明:2%次氯酸钠2 mL和0.1%氯化汞8mL组成的消毒液对老虎须种子消毒灭菌效果最好;温度对老虎须种子发芽率的影响不显著;最好的愈伤诱导培养基为MS+BA 0.2mg/L+NAA 0.1mg/L;MS+BA 3.0mg/L+NAA 0.1mg/L是最适宜的增殖培养基,MS+BA 0.5mg/L+IBA 0.5mg/L是最适宜的生根培养基。草炭∶珍珠岩为1∶1的基质移植练苗较好。
In order to rapidly propagate T.chantrieri seedlings and apply in actual production,sterile seedlings were gained after seed disinfection and inoculation,calli were induced with leaves and petioles,then enrichment culture,rooting culture and hardening-seedling transplantation were conducted.Results:2% sodium hypochlorite 2 mL plus 0.1% mercuric chloride 8 mL have the best sterilization effect on T.chantrieri seeds;temperature has no significant influence on seed germination rate;the best callus induction medium is MS+BA 0.2 mg/L+NAA 0.1 mg/L;the optimal enrichment medium is MS+BA3.0mg/L+NAA 0.1mg/L,and the optimal rooting medium is MS+BA 0.5mg/L+IBA 0.5mg/L,1∶1of peat and perlite is suitable for hardening-seedling transplantation.
In order to rapidly propagate T.chantrieri seedlings and apply in actual production,sterile seedlings were gained after seed disinfection and inoculation,calli were induced with leaves and petioles,then enrichment culture,rooting culture and hardening-seedling transplantation were conducted.Results:2% sodium hypochlorite 2 mL plus 0.1% mercuric chloride 8 mL have the best sterilization effect on T.chantrieri seeds;temperature has no significant influence on seed germination rate;the best callus induction medium is MS+BA 0.2 mg/L+NAA 0.1 mg/L;the optimal enrichment medium is MS+BA3.0mg/L+NAA 0.1mg/L,and the optimal rooting medium is MS+BA 0.5mg/L+IBA 0.5mg/L,1∶1of peat and perlite is suitable for hardening-seedling transplantation.
引文
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[7]高福洪,刘科伟,杨军,等.光照强度对老虎须生长的影响[J].安徽农业科学,2015,43(16):65-66.
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[9]何惠英,兰芹英.翦根薯的试管繁殖[J].广西农业生物科学,2002,21(2):108-110.
[2]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1985.
[3]何惠英,文彬,殷寿华.箭根薯种子发芽试验[J].中南林学院学报,2003,23(4):120-122.
[4]唐德英,何明荣.珍稀濒危药用植物箭根薯的研究进展[J].时珍国医国药,2009,20(7):1831-1833.
[5]云南省药物研究所.云南天然药物图鉴[M].昆明:云南科技出版社,2003.
[6]国家环境保护局,中国科学院植物研究所.中国珍稀濒危植物[M].上海:上海教育出版社,1989.
[7]高福洪,刘科伟,杨军,等.光照强度对老虎须生长的影响[J].安徽农业科学,2015,43(16):65-66.
[8]刘颂颂,叶永昌.药翦薯的组织培养及植株再生[J].植物生理学通讯,2002,38(3):254.
[9]何惠英,兰芹英.翦根薯的试管繁殖[J].广西农业生物科学,2002,21(2):108-110.