HPLC测定四川梓潼产葛根和粉葛中四种异黄酮含量的分析
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摘要
采用YMC-Pack ODS-A C18色谱柱(250 mm×4.6 mm,5μm)测定四川梓潼产葛根和粉葛中4种异黄酮(葛根素、3′-甲氧基葛根素、大豆苷和大豆苷元)含量。结果测得葛根素、3′-甲氧基葛根素、大豆苷和大豆苷元的线性范围分别为0.050～0.750、0.010～0.150、0.010～0.150和0.012～0.180μg,线性关系良好(r>0.999),平均加样回收率为96.52%～100.22%,RSD为0.75%～1.84%。该方法简便、准确、重现性好,适用于葛根中异黄酮含量的测定。梓潼产葛根中葛根素、3′-甲氧基葛根素、大豆苷和大豆苷元的含量明显高于粉葛,且梓潼产葛根和粉葛中大豆苷元含量明显高于其他产地的葛根和粉葛。
To determine the contents of four isoflavones of Pueraria lobata and Pueraria thomsonii in Sichuan Zitong by HPLC. The chromatographic conditions were YMC-Pack ODS-A C18 column(250 mm ×4.6 mm,5 μm). The linear range of puerarin, 3′-methoxy puerarin, daidzin and daidzein were 0.050～0.750, 0.010～0.150, 0.010～0.150 and 0.012～0.180 μg, respectively, and it showed a good linear relationship(r>0.999), the average recovery rate was between 96.52%～100.22%, and RSD was between 0.75% ～1.84%(n =6). This method is simple, accurate and reproducible. It is suitable for the determination of isoflavones in Puerarin. The contents of 4 isoflavones of P. lobata are obviously higher than P. thomsonii from Zitong, Sichuan Province. And the content of daidzein in P. lobata and P. thomsonii from Zitong is obviously higher than other habitat origins reported.
To determine the contents of four isoflavones of Pueraria lobata and Pueraria thomsonii in Sichuan Zitong by HPLC. The chromatographic conditions were YMC-Pack ODS-A C18 column(250 mm ×4.6 mm,5 μm). The linear range of puerarin, 3′-methoxy puerarin, daidzin and daidzein were 0.050～0.750, 0.010～0.150, 0.010～0.150 and 0.012～0.180 μg, respectively, and it showed a good linear relationship(r>0.999), the average recovery rate was between 96.52%～100.22%, and RSD was between 0.75% ～1.84%(n =6). This method is simple, accurate and reproducible. It is suitable for the determination of isoflavones in Puerarin. The contents of 4 isoflavones of P. lobata are obviously higher than P. thomsonii from Zitong, Sichuan Province. And the content of daidzein in P. lobata and P. thomsonii from Zitong is obviously higher than other habitat origins reported.
引文
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[3]王臻,张丽,王炎.葛根研究进展[J].上海医药,2007,28(10):464.
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[11]王治平,李卫民,高英,等.HPLC同时测定不同产地葛根中6种主要异黄酮类成分的含量[J].中国实验方剂学杂志,2013,19(8):125-128.
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