超高效色谱柱分析测定中草药中低聚果糖的方法研究
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摘要
采用超高效色谱柱建立高效液相色谱法测定中草药中低聚果糖的方法。通过小颗粒色谱柱的高塔板数分离能力,有效排除中草药基质干扰,并准确定量中草药中低聚果糖的含量。结果表明,建立的测试方法下,低聚果糖中蔗果三糖、蔗果四糖、蔗果五塘的标曲线性关系良好,相关系数均在0. 999以上,检出限均为0. 3 g/100 g、回收率在95%～105%之间,相对偏差均小于4%。该测定方法操作简单、高效,适合中草药中低聚果糖含量的检测分析。
HPLC method was established for the determination of fructo-oligofructose in Chinese medicinal herb.The content of fructo-oligofructose in Chinese medicinal herb was accurately quantified by HPLC with small particle chromatographic column that could eliminate the interferences of matrix. The results showed that the standard curves of 1-kestose,nistose and 1 F-fructofuranosylnystose had good linear relation,the correlation coefficients were all above 0. 999,the limit of quantitation were all 0. 3 g/100 g,the recoveries were between 95% ～ 105%,and RSD were all less than 4%.The method was simple and efficient,and suitable for the determination of fructo-oligofructose in Chinese medicinal herb.
HPLC method was established for the determination of fructo-oligofructose in Chinese medicinal herb.The content of fructo-oligofructose in Chinese medicinal herb was accurately quantified by HPLC with small particle chromatographic column that could eliminate the interferences of matrix. The results showed that the standard curves of 1-kestose,nistose and 1 F-fructofuranosylnystose had good linear relation,the correlation coefficients were all above 0. 999,the limit of quantitation were all 0. 3 g/100 g,the recoveries were between 95% ～ 105%,and RSD were all less than 4%.The method was simple and efficient,and suitable for the determination of fructo-oligofructose in Chinese medicinal herb.
引文
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[7]刘芸,丁涛,徐素丽,等.高效液相色谱-四级杆/静电场轨道阱高分辨质谱测定奶粉中的低聚果糖[J].色谱,2015,33(10):1040-1045.
[8]魏远安,郑惠玲,吴少辉,等.高效离子色谱法分离、检测低聚果糖[J].食品科学,2015,36(14):151-155.
[9]丁洪流,李灿,金萍,等.高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇[J].色谱,2013,31(8):804-808.
[10]耿丽娟,黄峻榕,冯峰,等.高效阴离子交换色谱-脉冲安培检测法测定乳粉中的低聚果糖[J].色谱,2014,32(12):1380-1384.
[11]张丽君.低聚果糖检测分析方法对比及优劣性分析[J].食品工业科技,2015,36(23):395-399.
[2]国家质量监督检验检疫总局,国家标准化管理委员会. GB/T23528-2009低聚果糖[S].
[3] Dominguez A L,Rodrigues L R,Lima N M,et al. An Overview of the Recent Developments on Fructo-oligosaccharide Production and Applications[J]. Food Bioprocess Technol,2013,6(12):1-14.
[4] Costa T G, Abreu G C, Guimaraes A B B, et al. Fructooligosaccharide effects on serum cholesterol levels. An overview[J].Acta Cirurgica Brasileira,2015,30(5):366-370.
[5] Erdos B,Grachten M,Czermak P,et al. Artificial Neural NetworkAssisted Spectrophotometric method for Monitoring Fructooligosaccharides Production[J]. Food Bioprocess Technol,2018,11(2):1-9.
[6] Matusek A,Meresz P,Le T K D,et al. Effect of temperature and p H on the degradation of fructo-oligosaccharides[J]. European Food Research and Technology,2009,228(3):355-365.
[7]刘芸,丁涛,徐素丽,等.高效液相色谱-四级杆/静电场轨道阱高分辨质谱测定奶粉中的低聚果糖[J].色谱,2015,33(10):1040-1045.
[8]魏远安,郑惠玲,吴少辉,等.高效离子色谱法分离、检测低聚果糖[J].食品科学,2015,36(14):151-155.
[9]丁洪流,李灿,金萍,等.高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇[J].色谱,2013,31(8):804-808.
[10]耿丽娟,黄峻榕,冯峰,等.高效阴离子交换色谱-脉冲安培检测法测定乳粉中的低聚果糖[J].色谱,2014,32(12):1380-1384.
[11]张丽君.低聚果糖检测分析方法对比及优劣性分析[J].食品工业科技,2015,36(23):395-399.