姜黄素对糖尿病大鼠子宫内膜整合素β3、 LIF表达水平的影响
|
摘要
目的观察姜黄素在治疗糖尿病及其并发症过程中对整合素β3、白细胞抑制因子(leukemia inhibitory factor,LIF)表达水平的影响。方法将雌性SD大鼠30只随机分为3组进行灌胃治疗,分别为:正常组10只,选用食用玉米油灌胃10周2.5 mL/kg;糖尿病模型组10只,选用食用玉米油灌胃10周2.5 mL/kg;姜黄素治疗组10只,选用姜黄素化合物混悬液灌胃10周每日250 mg/kg。10周后,按雌雄比例3∶2合笼,次日晨查雌鼠阴道,发现阴栓者计为妊娠第1天,雌性大鼠均于妊娠第4天取材。分别检测血糖、胰岛素,采用HE染色观察子宫内膜上皮的形态结构,运用免疫组织化学和免疫印迹实验法分析各组大鼠子宫内膜整合素β3、 LIF表达水平。结果糖尿病组与正常组比较,其血糖和胰岛素明显升高,子宫内膜整合素β3和LIF表达量明显减少,姜黄素治疗组与糖尿病组比较,其血糖和胰岛素明显降低,子宫内膜整合素β3和LIF表达量明显升高。结论姜黄素可降低血糖和胰岛素,增加子宫内膜整合素β3和LIF蛋白的表达,最终改善子宫内膜容受性。
Objective To investigate the effect of curcumin on the expression of integrin β3 and leukemia inhibitory factor(LIF) in endometrium of diabetic rats. Methods Thirty female Sprague-Dawley rats were divided into control group,Diabetes model group and curcumin compound group randomly. The normal controls and diabetes model group served with edible corn oil 2.5 mL/kg for 10 weeks, while the curcumin compound group were treated with 250 mg·kg~(-1)·d~(-1) curcumin compound suspension for the same time. After 10 weeks, the ratio of male to female was 3∶2 for mating. The vagina of the female was checked next day. The fetus was found to be the first day of pregnancy and were sacrificed in the fourth day. Blood glucose and insulin were measured separately. The morphology of endometrial epithelium were observed by HE staining and immunohistochemistry. Western blot analysis were used to analyze the changing of the endometrial integrin β3 and LIF after treatment with curcumin for 10 weeks. Results Compared with the normal group, the blood glucose and insulin were significantly increased in the diabetic group(P<0.05), and the expression of integrin β3 and LIF in the endometrium were significantly decreased(P<0.05). Compared with the diabetes group, the blood glucose and insulin were significantly lower(P<0.05), and the expression of integrin β3 and LIF in the endometrium were significantly increased(P<0.05) in the curcumin compound treatment group. Conclusion Curcumin can lower blood sugar and insulin, increase the expression of endometrial integrin β3 and LIF protein, and finally improve endometrial receptivity.
Objective To investigate the effect of curcumin on the expression of integrin β3 and leukemia inhibitory factor(LIF) in endometrium of diabetic rats. Methods Thirty female Sprague-Dawley rats were divided into control group,Diabetes model group and curcumin compound group randomly. The normal controls and diabetes model group served with edible corn oil 2.5 mL/kg for 10 weeks, while the curcumin compound group were treated with 250 mg·kg~(-1)·d~(-1) curcumin compound suspension for the same time. After 10 weeks, the ratio of male to female was 3∶2 for mating. The vagina of the female was checked next day. The fetus was found to be the first day of pregnancy and were sacrificed in the fourth day. Blood glucose and insulin were measured separately. The morphology of endometrial epithelium were observed by HE staining and immunohistochemistry. Western blot analysis were used to analyze the changing of the endometrial integrin β3 and LIF after treatment with curcumin for 10 weeks. Results Compared with the normal group, the blood glucose and insulin were significantly increased in the diabetic group(P<0.05), and the expression of integrin β3 and LIF in the endometrium were significantly decreased(P<0.05). Compared with the diabetes group, the blood glucose and insulin were significantly lower(P<0.05), and the expression of integrin β3 and LIF in the endometrium were significantly increased(P<0.05) in the curcumin compound treatment group. Conclusion Curcumin can lower blood sugar and insulin, increase the expression of endometrial integrin β3 and LIF protein, and finally improve endometrial receptivity.
引文
[1] 魏瑞瑞,安力彬,李晶,等.女性不孕症患者生活质量现状调查及影响因素分析[J].中国妇幼保健,2017,32(6):1262-1265.
[2] 姜海英,李静,杨丽丽,等.妊娠期糖尿病早期对血清Nesfatin-1、IL-6、TNF-α及APN表达水平的影响[J].河北医科大学学报,2018,39(8):934-937.
[3] 耿会转,周忠明,邓亚丽,等.解郁育胞方对慢性应激致大鼠子宫内膜低容受性的机制研究[J].中药药理与临床,2016,32(1):152-156.
[4] Lyu BY,Li D,Zhang HL,et al.Effect and regulation mechanism of Chinese Bushen Huoxue prescriptions on endometrial receptivity[J].Zhongguo Zhong Yao Za Zhi,2018,43(10):2014-2019.
[5] 龚淼,李莉,包宪霞,等.姜黄素对体外培养的人食管癌Ec-109细胞增殖和凋亡的影响[J].河北医科大学学报,2012,33(2):125-128.
[6] Aghajanova L,Altm?e S,Bjuresten K,et al.Disturbances in the LIF pathway in the endometrium among women with unexplained infertility[J].Fertil Steril,2009,91(6):2602-2610.
[7] Lessey BA,Ilesanmi AO,Lessey MA,et al.Luminal and glandular endometrial epithelium express integrins differentially throughout the menstrual cycle:implications for implantation,contraception,and infertility[J].Am J Reprod Immunol,1996,35(3):195-204.
[8] Catalano PM,Shankar K.Obesity and pregnancy:mechanisms of short term and long term adverse consequences for mother and child[J].BMJ,2017,356:j1.
[9] 余军辉,应柳青,王珺.抑抗汤对子宫内膜容受性作用的影响[J].现代实用医学,2018,30(8):994-996.
[10] 肖美玲,张汉青.右归丸对肾阳虚型不孕症患者卵泡发育及子宫内膜容受性的影响[J].现代中西医结合杂志,2018,27(26):2902-2904.
[11] 黄紫微.疏肝补肾法对慢性应激模型小鼠子宫内膜容受性的调节及作用研究[D].郑州:河南中医药大学,2017.
[12] Gutierres VO,Assis RP,Arcaro CA,et al.Curcumin improves the effect of a reduced insulin dose on glycemic control and oxidative stress in streptozotocin-diabetic rats[J].Phytother Res,2019,33(4):976-988.
[13] 李向东,王秀丽.胚胎干细胞自我更新与诱导分化[J].大连医科大学学报,2017,39(5):417-422,432.
[14] Zhao L,Xu Y,Duo X,et al A probe into effect of Shoutai pill on endometrial receptivity[J].Exp Ther Med,2018,15(4):3765-3768.
[15] 李秀然.助孕增膜方对肾虚胚胎着床障碍小鼠子宫内膜容受性及相关因子时序表达的影响[J].广州中医药大学学报,2018,35(6):1058-1065.
[16] 马彩辉,古少君.体外受精-胚胎移植失败者子宫内膜整合素β3及细胞间隙连接蛋白43的表达研究[J].中国医刊,2018,53(6):623-625.
[2] 姜海英,李静,杨丽丽,等.妊娠期糖尿病早期对血清Nesfatin-1、IL-6、TNF-α及APN表达水平的影响[J].河北医科大学学报,2018,39(8):934-937.
[3] 耿会转,周忠明,邓亚丽,等.解郁育胞方对慢性应激致大鼠子宫内膜低容受性的机制研究[J].中药药理与临床,2016,32(1):152-156.
[4] Lyu BY,Li D,Zhang HL,et al.Effect and regulation mechanism of Chinese Bushen Huoxue prescriptions on endometrial receptivity[J].Zhongguo Zhong Yao Za Zhi,2018,43(10):2014-2019.
[5] 龚淼,李莉,包宪霞,等.姜黄素对体外培养的人食管癌Ec-109细胞增殖和凋亡的影响[J].河北医科大学学报,2012,33(2):125-128.
[6] Aghajanova L,Altm?e S,Bjuresten K,et al.Disturbances in the LIF pathway in the endometrium among women with unexplained infertility[J].Fertil Steril,2009,91(6):2602-2610.
[7] Lessey BA,Ilesanmi AO,Lessey MA,et al.Luminal and glandular endometrial epithelium express integrins differentially throughout the menstrual cycle:implications for implantation,contraception,and infertility[J].Am J Reprod Immunol,1996,35(3):195-204.
[8] Catalano PM,Shankar K.Obesity and pregnancy:mechanisms of short term and long term adverse consequences for mother and child[J].BMJ,2017,356:j1.
[9] 余军辉,应柳青,王珺.抑抗汤对子宫内膜容受性作用的影响[J].现代实用医学,2018,30(8):994-996.
[10] 肖美玲,张汉青.右归丸对肾阳虚型不孕症患者卵泡发育及子宫内膜容受性的影响[J].现代中西医结合杂志,2018,27(26):2902-2904.
[11] 黄紫微.疏肝补肾法对慢性应激模型小鼠子宫内膜容受性的调节及作用研究[D].郑州:河南中医药大学,2017.
[12] Gutierres VO,Assis RP,Arcaro CA,et al.Curcumin improves the effect of a reduced insulin dose on glycemic control and oxidative stress in streptozotocin-diabetic rats[J].Phytother Res,2019,33(4):976-988.
[13] 李向东,王秀丽.胚胎干细胞自我更新与诱导分化[J].大连医科大学学报,2017,39(5):417-422,432.
[14] Zhao L,Xu Y,Duo X,et al A probe into effect of Shoutai pill on endometrial receptivity[J].Exp Ther Med,2018,15(4):3765-3768.
[15] 李秀然.助孕增膜方对肾虚胚胎着床障碍小鼠子宫内膜容受性及相关因子时序表达的影响[J].广州中医药大学学报,2018,35(6):1058-1065.
[16] 马彩辉,古少君.体外受精-胚胎移植失败者子宫内膜整合素β3及细胞间隙连接蛋白43的表达研究[J].中国医刊,2018,53(6):623-625.