两种大米品系组方白虎汤对酵母致热大鼠解热作用的影响
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摘要
目的:探究粳米组方白虎汤与糯米组方白虎汤对酵母致热大鼠解热作用的影响及其机制。方法:将40只SD大鼠随机分为5组,分别为正常组、模型组、布洛芬组(0.1 mg·kg-1)、粳米组(20 mg·kg-1)和糯米组(20 mg·kg-1)。除正常组外,其他4组皮下注射干酵母复制大鼠发热模型。每间隔1 h测量大鼠体温情况,共12 h,计算体温变化值△T、最大发热净增值△Tmax和6 h体温反应指数(TRI6);造模后6 h开始给药,给药6 h后处死大鼠,取血清检测血清中IL-10、IL-12、TNF-α的表达水平。结果:与正常组比较,模型组大鼠体温明显上升,7 h达到高峰期(P<0.05);粳米组给药4 h后开始起效(P<0.05),能明显降低发热大鼠ΔTmax、TRI6(P<0.01或P<0.05),血清中IL-12、TNF-α含量明显低于模型组(P<0.05);糯米组给药5 h后开始起效(P<0.05),能明显降低TRI6(P<0.05),血清中TNF-α含量明显低于模型组(P<0.01)。结论:粳米和糯米组方白虎汤均能降低酵母致热大鼠体温,其作用可能与调节发热炎性因子有关,且粳米组方白虎汤的作用更佳。
Objective: To explore the antipyretic effect and its mechanism of Baihu decoction composed of Jingmi(japonica rice) or Nuomi(glutinous rice) in yeast-induced rats. Methods: A total of 40 SD rats were randomly divided into 5 groups: normal group, model group, ibuprofen group(0.1 mg·kg-1), Jingmi(japonica rice)group(20 mg·kg-1) and Nuomi(glutinous rice) group(20 mg·kg-1). Except the normal group, the other 4 groups were induced by injection of yeast suspension subcutaneously. The temperature of rats was measured at 1 h intervals, with a total of 12 h. The temperature change value(△T), the max of temperature net added value(△Tmax)and thermal response index in 6 h(TRI6) were calculated. After 6 h of model, the rats were respectively administered. After 6 h of intragastric gavage, the rats were killed, and the serum IL-10, IL-12 and TNF-α were detected. Results: Compared with the normal group, the temperature of model group obviously rose and reached peak after 7 h(P<0.05). Compared with the model group, after 4 h of administered, Jingmi(japonica rice) group began to take antipyretic effect(P<0.05), significantly reduced △Tmaxand TRI6(P<0.01 or P<0.05) and significantly decreased IL-12 and TNF-α(P<0.05). After 5 h of administered, Nuomi(glutinous rice) group began to take antipyretic effect(P <0.05), just reduced TRI6(P <0.05) and decreased TNF-α(P <0.05). Conclusion: Baihu decoction with Jingmi(japonica rice) or Nuomi(glutinous rice) could reduce the temperature of yeast-induced rats, which may be related to regulate inflammatory cytokines. Baihu decoction with Jingmi(japonica rice) had a better antipyretic effect.
Objective: To explore the antipyretic effect and its mechanism of Baihu decoction composed of Jingmi(japonica rice) or Nuomi(glutinous rice) in yeast-induced rats. Methods: A total of 40 SD rats were randomly divided into 5 groups: normal group, model group, ibuprofen group(0.1 mg·kg-1), Jingmi(japonica rice)group(20 mg·kg-1) and Nuomi(glutinous rice) group(20 mg·kg-1). Except the normal group, the other 4 groups were induced by injection of yeast suspension subcutaneously. The temperature of rats was measured at 1 h intervals, with a total of 12 h. The temperature change value(△T), the max of temperature net added value(△Tmax)and thermal response index in 6 h(TRI6) were calculated. After 6 h of model, the rats were respectively administered. After 6 h of intragastric gavage, the rats were killed, and the serum IL-10, IL-12 and TNF-α were detected. Results: Compared with the normal group, the temperature of model group obviously rose and reached peak after 7 h(P<0.05). Compared with the model group, after 4 h of administered, Jingmi(japonica rice) group began to take antipyretic effect(P<0.05), significantly reduced △Tmaxand TRI6(P<0.01 or P<0.05) and significantly decreased IL-12 and TNF-α(P<0.05). After 5 h of administered, Nuomi(glutinous rice) group began to take antipyretic effect(P <0.05), just reduced TRI6(P <0.05) and decreased TNF-α(P <0.05). Conclusion: Baihu decoction with Jingmi(japonica rice) or Nuomi(glutinous rice) could reduce the temperature of yeast-induced rats, which may be related to regulate inflammatory cytokines. Baihu decoction with Jingmi(japonica rice) had a better antipyretic effect.
引文
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[2]中国社会科学院语言研究所词典编辑室.现代汉语词典[Z].5版.北京:商务印书馆,2005.
[3]杜双奎,杨红丹,于修烛,等.商品粳米、籼米、糯米品质特性和糊化特性比较研究[J].食品科学,2010,31(5):78-81.
[4]农业部行业标准食用粳米NY/T594-2002[J].中国稻米,2003,9(4):41-42.
[5]仝小林,穆兰澄,姬航宇,等.《伤寒论》药物剂量考[J].中医杂志,2009,50(4):368-372.
[6]万红叶,孔祥英,李晓敏,等.不同寒热药性中药对酵母致热大鼠体温调节及温度敏感瞬时感受器电位离子通道(TRPs)蛋白的影响[J].中国中药杂志,2014,39(19):3813-3818.
[7]左泽平,王志斌,郭玉东,等.常用大鼠发热模型研究[J].中国比较医学杂志,2012,22(2):52-57.
[8]黄启福.病理学[M].北京:科学出版社,2007:127-131.
[9]Conti B,Tabarean I,Andrei C,et al.Cytokines and fever[J].Front Biosci,2004,9(12):1433-1449.
[10]Dinarello CA.Infection,fever,and exogenous and endogenous pyrogens:some concepts have changed[J].J Endotoxin Res,2004,10(4):201-222.
[11]赵志超,岳屹囡.细胞因子与临床疾病[M].海口:海南出版社,2008:7.
[12]Wilke C M,Wei S,Wang L,et al.Dual biological effects of the cytokines interleukin-10 and interferon-γ[J].Cancer Immunology,Immunotherapy,2011,60(11):1529-1541.
[13]顾秋萍,白爱平.白介素-10与炎症性肠病[J].世界华人消化杂志,2011,19(1):57-61.