低温胁迫对设施小青菜叶片抗衰老特性的影响
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摘要
以小青菜品种"新场青一号"为试材,利用人工环境控制试验研究的方法,研究了低温胁迫对设施小青菜的叶绿素含量、保护酶活性、细胞膜透性及抗衰老特性的影响,以期为小青菜设施种植的低温灾害防控提供指导。结果表明:在-6~0℃范围内,小青菜叶片的叶绿素含量随着低温胁迫强度的加剧和持续时间的延长而明显下降;叶片组织的超氧化物歧化酶(SOD)活性随着胁迫温度的降低而不断升高,过氧化物酶(POD)活性和过氧化氢酶(CAT)活性随着处理时间的延长,均表现出先升高后降低的趋势;丙二醛(MDA)含量则表现出了先升高后降低再升高的特点;叶片的相对电导率随着胁迫温度的降低而升高,而温度越低,增长幅度越大。研究认为,小青菜叶片的抗衰老特性随气温降低而降低,细胞膜透性变得越差,-4℃持续9h时,细胞活性受到不可恢复的伤害,因此,该温度为小青菜低温胁迫产生结构性伤害的临界温度。
Brassica campestris L.'Xinchangqing 1'was used as test material,the effects of low temperature stress on the chlorophyll,protective enzyme activity,and cell membrane permeability of Brassica chinensis were studied by using artificial environment control experiment.The results showed that the chlorophyll content of Brassicachinensis leaves decreased with the increasing of low temperature stress intensity and prolonged duration in the range from -6 ℃ to 0 ℃.SOD activity of leaf tissue increased with the decrease of stress temperature,the activities CAT and POD increased first and then decreased with the prolonging of treatment time.The content of MDA increased first,then decreased and increased again.The relative electrical conductivity of leaves increased with the decrease of temperature,while the lower the temperature,the greater the growth rate.The results showed that the anti-aging properties of the leaves of Brassica chinensis decreased with the decrease of temperature and the cell membrane permeability became worse.When the temperature was -4 ℃for 9 hours,the cell viability was irreversibly damaged.Therefore,it was the critical temperature of structural damage of Brassica chinensis under low temperature stress.
Brassica campestris L.'Xinchangqing 1'was used as test material,the effects of low temperature stress on the chlorophyll,protective enzyme activity,and cell membrane permeability of Brassica chinensis were studied by using artificial environment control experiment.The results showed that the chlorophyll content of Brassicachinensis leaves decreased with the increasing of low temperature stress intensity and prolonged duration in the range from -6 ℃ to 0 ℃.SOD activity of leaf tissue increased with the decrease of stress temperature,the activities CAT and POD increased first and then decreased with the prolonging of treatment time.The content of MDA increased first,then decreased and increased again.The relative electrical conductivity of leaves increased with the decrease of temperature,while the lower the temperature,the greater the growth rate.The results showed that the anti-aging properties of the leaves of Brassica chinensis decreased with the decrease of temperature and the cell membrane permeability became worse.When the temperature was -4 ℃for 9 hours,the cell viability was irreversibly damaged.Therefore,it was the critical temperature of structural damage of Brassica chinensis under low temperature stress.
引文
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[15]张帆,郁继华,颉建明,等.外源ALA和Spd对低温弱光下辣椒幼苗光合作用及抗氧化系统的影响[J].中国农业科学,2013,46(11):2298-2306.
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[24]武辉,周艳飞,侯丽丽,等.低温弱光胁迫对棉花幼苗叶绿素荧光特性及能量分配的影响[J].新疆农业科学,2012,49(3):393-399.
[25]逮明辉,娄群峰,陈尽枫.黄瓜的冷害及耐冷性[J].植物学报,2004,21(5):575-586.
[26]SHIMAZAKI K,SAKAKI T,KONDO N,et al.Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO2-fumigated leaves of spinach[J].Plant&Cell Physiology,1980,21(7):1193-1204.
[27]张翠利,付丽娜,杨小云,等.活性氧自由基与细胞衰老关系的研究进展[J].广州化工,2015(19):5-7.
[28]MARCELA S,SUSANE P.Oxygen radical generation by isolated microsomes from soybean seedling[J].Plant Physiology,1992,100(3):1263-1268.
[29]JOHN G S.Oxygen stress and superoxide dismutase[J].Plant Physiology,1993,101(1):7-12.
[30]MCCORD J M,FRIDOVICH J.Superoxide dismutase:An enzymic function for erythrocuprein(Hemocaprein)[J].J Biol Chem,1969,224:6049-6055.
[31]KUK Y I,SHIN J S,BURGOS N R,et al.Antioxidative enzymes offer protection from chilling damage in rice plants[J].Crop Sci,2003,43(6):2109-2117.
[32]ISMAIL T,BOR M,OZDEMIR F,et al.Differential responses of lipid-peroxidationand antioxidants in the leaves of droughttolerant P.acutifolius,gray and drought-sensitive P.vulgaris L.subjected to polyethylene glycol mediatedwater stress[J].Plant Science,2005,168(1):223-231.
[33]DEMIRAL T,TRKAN I.Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment[J].Journal Plant Physiology,2004,161(10):1089-1100.
[34]聂庆娟,孟朝,梁海永.低温胁迫对4种常绿阔叶植物膜脂过氧化及保护酶活性的影响[J].植物研究,2007,27(5):578-581.
[35]段曦,魏佑营,曹克友,等.低温弱光胁迫及恢复过程对CMS三系辣椒幼苗保护酶活性与渗透调节物质的影响[J].天津农业科学,2012,18(1):11-15.
[36]沈静,杨青川,曹致中,等.低温胁迫对野牛草细胞膜和保护酶活性的影响[J].中国草地学报,2010,15(2):98-102.
[37]康冬鸽,李瑞梅,胡新文,等.低温胁迫下木薯几种保护酶活性变化及其与耐寒性的关系[J].热带作物学报,2009,30(7):908-911.
[38]李建设,耿广东,程智慧.低温胁迫对茄子幼苗抗寒性生理生化指标的影响[J].西北农林科技大学学报(自然科学版),2003,31(1):90-92,96.
[2]王春萍,黄启中,雷开荣,等.低温弱光下辣椒幼苗叶绿素荧光特性及其与品种耐性的关系[J].园艺学报,2015,42(9):1798-1806.
[3]梁芳,郑成淑,孙宪芝,等.低温弱光胁迫及恢复对切花菊光合作用和叶绿素荧光参数的影响[J].应用生态学报,2010,21(1):29-35.
[4]JEONG S W,CHOI S M,LEE D S,et al.Differential susceptibility of photosynthesis to light-chilling stress in rice(Oryza sativa L.)depends on the capacity for photochemical dissipation of light[J].Molecules&Cells,2002,13(3):419-428.
[5]WOO N S,BADGER M R,POGSON B J.A rapid,non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescence[J].Plant Methods,2008,4(1):27.
[6]何勇,符庆功,朱祝军.低温弱光对辣椒叶片光合作用、叶绿素荧光猝灭及光能分配的影响[J].核农学报,2013,27(4):479-486.
[7]ZHOU J S,XIONG H Y,YANG C J,et al.Chemical compositions of volatile oil from fruiting body of Armillaria luteovirens[J].Agricultural Science&Technology,2008,9(2):90-92.
[8]ROY C S,CHOUDHURI M A.Hydrogen peroxide metabolism as an index of water stress tolerance in jute[J].Physiologia Plantarum,2010,65(4):476-480.
[9]卢佳华,张敏,谢晶,等.低温胁迫下黄瓜果实膜透性和保护酶活性的变化[J].广东农业科学,2012,39(22):42-44.
[10]FRIDOVICH I.The biology of oxygen radical[J].Science,1978,201(4359):875-880.
[11]LUO X Y,FENG C J,LI H,et al.Study on changes of SOD and proline activity during low temperature stress on Medicago sativa L.cv.Zhaodong[J].Grassland of China,2004,26(4):79-81.
[12]XIA M,LIU Y X,ALA M,et al.Relationship between chilling resistance and POD isoenzyme on alfalfa leaves[J].Grassland of China,2003,25(4):41-45.
[13]孟雅宁,严立斌,范妍芹.低温弱光对不同品种甜(辣)椒幼苗抗氧化酶活性与质膜透性的影响[J].山东农业科学,2016,48(11):38-42.
[14]刘伟,艾希珍,梁文娟,等.低温弱光下水杨酸对黄瓜幼苗光合作用及抗氧化酶活性的影响[J].应用生态学报,2009,20(2):441-445.
[15]张帆,郁继华,颉建明,等.外源ALA和Spd对低温弱光下辣椒幼苗光合作用及抗氧化系统的影响[J].中国农业科学,2013,46(11):2298-2306.
[16]DHINDSA R S.Inhibition of protein synthesis by products of lipid peroxidation[J].Phytochemistry,1982,21(2):309-313.
[17]RABINOWITCH H D,SKLAN D.Sunscald tolerance in tomatoes:Role of superoxide dismutase[J].Planta,1980,148(2):162-167.
[18]CHANCE B,MAEHLY A C.Assay of catalase and peroxidase[M]//Methods of Enzymology.New York:Education Press,1954,55(1):755-764.
[19]LI H S.Plant physiological and biochemical priciples and experimental techniques[M].Beijing:Higher Education Press,1955:164-167.
[20]BRADFORD M M.A Rapid and sensitive method for the determination of microgram quantities of protein utilizing the prinple of protein-dye binding[J].Anlytical Biochemistry,2015,72(s1-2):248-255.
[21]王晶,徐志英,盛云燕.低温对绿萝幼苗叶片氮素及叶绿素含量的影响[J].现代农业科技,2011(15):208-209.
[22]白青华,郭晓冬,王萍,等.低温对辣椒幼苗叶片氮素及叶绿素含量的影响[J].甘肃农业大学学报,2009,44(6):48-51.
[23]和红云,薛琳,田丽萍,等.低温胁迫对甜瓜幼苗叶绿素含量及荧光参数的影响[J].北方园艺,2008(4):13-16.
[24]武辉,周艳飞,侯丽丽,等.低温弱光胁迫对棉花幼苗叶绿素荧光特性及能量分配的影响[J].新疆农业科学,2012,49(3):393-399.
[25]逮明辉,娄群峰,陈尽枫.黄瓜的冷害及耐冷性[J].植物学报,2004,21(5):575-586.
[26]SHIMAZAKI K,SAKAKI T,KONDO N,et al.Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO2-fumigated leaves of spinach[J].Plant&Cell Physiology,1980,21(7):1193-1204.
[27]张翠利,付丽娜,杨小云,等.活性氧自由基与细胞衰老关系的研究进展[J].广州化工,2015(19):5-7.
[28]MARCELA S,SUSANE P.Oxygen radical generation by isolated microsomes from soybean seedling[J].Plant Physiology,1992,100(3):1263-1268.
[29]JOHN G S.Oxygen stress and superoxide dismutase[J].Plant Physiology,1993,101(1):7-12.
[30]MCCORD J M,FRIDOVICH J.Superoxide dismutase:An enzymic function for erythrocuprein(Hemocaprein)[J].J Biol Chem,1969,224:6049-6055.
[31]KUK Y I,SHIN J S,BURGOS N R,et al.Antioxidative enzymes offer protection from chilling damage in rice plants[J].Crop Sci,2003,43(6):2109-2117.
[32]ISMAIL T,BOR M,OZDEMIR F,et al.Differential responses of lipid-peroxidationand antioxidants in the leaves of droughttolerant P.acutifolius,gray and drought-sensitive P.vulgaris L.subjected to polyethylene glycol mediatedwater stress[J].Plant Science,2005,168(1):223-231.
[33]DEMIRAL T,TRKAN I.Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment[J].Journal Plant Physiology,2004,161(10):1089-1100.
[34]聂庆娟,孟朝,梁海永.低温胁迫对4种常绿阔叶植物膜脂过氧化及保护酶活性的影响[J].植物研究,2007,27(5):578-581.
[35]段曦,魏佑营,曹克友,等.低温弱光胁迫及恢复过程对CMS三系辣椒幼苗保护酶活性与渗透调节物质的影响[J].天津农业科学,2012,18(1):11-15.
[36]沈静,杨青川,曹致中,等.低温胁迫对野牛草细胞膜和保护酶活性的影响[J].中国草地学报,2010,15(2):98-102.
[37]康冬鸽,李瑞梅,胡新文,等.低温胁迫下木薯几种保护酶活性变化及其与耐寒性的关系[J].热带作物学报,2009,30(7):908-911.
[38]李建设,耿广东,程智慧.低温胁迫对茄子幼苗抗寒性生理生化指标的影响[J].西北农林科技大学学报(自然科学版),2003,31(1):90-92,96.