首乌藤总黄酮提取工艺优化及抗氧化性研究
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摘要
以首乌藤为原料,超纯水为提取溶剂,通过单因素和正交试验对超声波辅助提取首乌藤总黄酮工艺进行优化;并通过考察首乌藤总黄酮对1,1-苯基-2-苦基肼自由基(DPPH·)和羟基自由基(·OH)的清除率来评价其抗氧化性。结果表明,首乌藤总黄酮的最佳提取工艺参数为超声功率350W,超声温度60℃,超声时间120min,料液比1∶50(g∶mL)。在此优化条件下,总黄酮的含量为1.32mg/g。总黄酮质量浓度为0.0544mg/mL时,对DPPH·和·OH的清除率分别可达74.36%和62.29%,说明首乌藤总黄酮具有较强的抗氧化性。
Using Polygoni multifori as raw material and ultrapure water as extracting solvent, on the basis of single factor tests, the optimum conditions of ultrasonic-assisted extraction of total flavonoids were optimized by orthogonal tests. Meanwhile, the antioxidant activity of total flavonoids from P. multifori was evaluated by DPPH· and ·OH scavenging capacity. Results showed that the optimal extraction conditions of total flavonoids were as follows: ultrasonic power 350 W, ultrasonic temperature 60 ℃, time 120 min, and solid to liquid ratio 1∶50(g∶ml). Under these conditions, the total flavonoids content was 1.32 mg/g. When the total flavonoids concentration was 0.0544 mg/ml, the scavenging rate of total flavonoids on DPPH·and·OH were 74.36% and 62.29%, which indicated that the total flavonoids of P. multifori had strong antioxidant activity.
Using Polygoni multifori as raw material and ultrapure water as extracting solvent, on the basis of single factor tests, the optimum conditions of ultrasonic-assisted extraction of total flavonoids were optimized by orthogonal tests. Meanwhile, the antioxidant activity of total flavonoids from P. multifori was evaluated by DPPH· and ·OH scavenging capacity. Results showed that the optimal extraction conditions of total flavonoids were as follows: ultrasonic power 350 W, ultrasonic temperature 60 ℃, time 120 min, and solid to liquid ratio 1∶50(g∶ml). Under these conditions, the total flavonoids content was 1.32 mg/g. When the total flavonoids concentration was 0.0544 mg/ml, the scavenging rate of total flavonoids on DPPH·and·OH were 74.36% and 62.29%, which indicated that the total flavonoids of P. multifori had strong antioxidant activity.
引文
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[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2010:248.
[3]王付荣,周洪雷.何首乌及夜交藤化学成分及药理作用的研究进展[J].江西中医学院学报,2007,19(5):98-100.
[4]缑慧君,张朝凤,张勉.何首乌不同器官和组织中蒽醌和茋类化合物的分布[J].中国野生植物资源,2008(2):44-48.
[5]汲广全,杨娟,杨小生.夜交藤改善睡眠活性成分研究[J].中成药,2011,33(3):514.
[6]罗飞华,杨莉,张萍,等.杜仲叶中总黄酮抗氧化性能研究[J].应用化工,2016,45(5):858-859,865.
[7]HABTEMARIAM S.Flavonoids as inhibitors or enhancers of the cytotoxicity of tumor necrosis factor-αin L-929 tumor cells[J].J Nat Prod,1997,60(8):775-778.
[8]HU C Q,CHEN K,SHI Q,et al.Anti-AIDS a-gents,10.acacetin 7-O-D-galactopyranoside,an anti-HIV principle from Chrysanthemum morifolium and a structure activity correlation with some related flavonoids[J].J Nat Prod,1994,57(1):42-51.
[9]潘佳伟,闫雨,尹芹,等.表面活性剂辅助提取首乌藤中的总黄酮[J].生物加工过程,2014,12(3):32-36.
[10]肖扬,段玉峰.超声辅助提取首乌藤黄酮及其抗氧化活性的研究[J].农产品加工·学刊,2009(6):38-41.
[11]史俊燕.首乌藤黄酮的提取、分离纯化及生物活性研究[D].西安:陕西师范大学,2010.
[12]杨申明,王波,王振吉,等.金雀花总黄酮提取工艺优化及抗氧化性研究[J].浙江农业学报,2015,27(2):278-284.
[13]石青浩,李荣,姜子涛.食用鼠曲草黄酮的提取及体外抗氧化性研究[J].食品与生物技术学报,2013,32(3):307-312.
[14]RAZALI N,RAZAB R,JUNIT S M,et al.Radical scavenging and reducing properties of extracts of cashew shoots(Anacardium occidentale)[J].Food Chem,2008,111(1):38-44.
[15]MOHSE S M,AMMAR A S M.Total phenolic contents and antioxidant activity of corn tassel extracts[J].Food Chem,2009,112(3):595-598.
[16]王全泽,袁堂丰,刘磊磊,等.响应面法优化闪式提取罗汉松总黄酮及其抗氧化活性[J].精细化工,2018,35(1):65-71,148.
[17]CHEN R Z,LIU Z Q,ZHAO J M,et al.Antioxidant and immunological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu[J].Food Chem,2011,127(2):434-440.
[18]刘慧,张春岭,刘杰超,等.超声-果胶酶协同提取山楂类黄酮的工艺优化[J].食品与机械,2016,32(1):154-157.
[19]张吉祥,欧来良.正交试验法优化超声提取枣核总黄酮[J].食品科学,2012,33(4):18-21.
[20]杨申明,王振吉,戴玥.藤三七总黄酮的超声波辅助提取及其抗氧化性研究[J].保鲜与加工,2018,18(5):65-71.
[21]杨昱,白靖文,俞志刚.超声辅助提取技术在天然产物提取中的应用[J].食品与机械,2011,27(1):170-174.