菌株MB6对三七总皂苷的微生物转化及其转化机理的研究
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摘要
从刺五加表层土壤中分离得到的菌株MB6具有很高的β-葡萄糖苷酶活性,可以有效地将三七总皂苷转化为次级的代谢物。同时对转化路径和最佳转化条件进行了测定,在R2A液体培养基,pH为6.0,温度为25℃时,转化时间为2天时,稀有皂苷Rg3含量最高。经16S rDNA鉴定结果表明,该菌株属于巨型芽孢杆菌属。
Strain MB6 isolated from the surface soil of Acanthopanax,which have a higher β-glucosidase activity,can convert ginsenoside notoginsenosides efficiently to less polar metabolites.Also the biotransformation pathway and the strains MB6 for the transformation of ginsenosides conditions were optimized.The optimum medium of MB6 was R2A,The pH was 6.0,the optimum temperature was 25℃,the transformation time was 2 days,under which the content of rare ginsenoside Rg3 was the highest.Blast results of 16S rDNA gene sequence of the strain MB6 established that the strain MB6 belonged to Bacillus megaterium.
Strain MB6 isolated from the surface soil of Acanthopanax,which have a higher β-glucosidase activity,can convert ginsenoside notoginsenosides efficiently to less polar metabolites.Also the biotransformation pathway and the strains MB6 for the transformation of ginsenosides conditions were optimized.The optimum medium of MB6 was R2A,The pH was 6.0,the optimum temperature was 25℃,the transformation time was 2 days,under which the content of rare ginsenoside Rg3 was the highest.Blast results of 16S rDNA gene sequence of the strain MB6 established that the strain MB6 belonged to Bacillus megaterium.
引文
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[16]Fu Y,Yin Z H,Yin C R.Biotransformation of ginsenoside Rb1 to ginsenoside Rg3 by endophytic bacterium Burkholderia sp.GE 17-7 isolated from Panax ginseng[J].Journal of Applied Microbiology,2017,122(6):1579~1585.
[17]赵林果,孟鹏,李丽娟,余世袁.利用七叶灵显色技术检验和判断β-葡萄糖苷酶的研究[J].食品与发酵工业,2008,34(12):163~166.
[18]包名家,周维民,孟庆敏.纸片法快速细菌七叶灵水解试验的研究[J].职业与健康,2003,19(10):67~69.
[2]龙全江,郁洋.RP-HPLC法测定血塞通注射液中三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1[J].现代中药研究与实践,2007,21(1):43~45.
[3]宋建平,曾江,崔学明,等.三七根茎的化学成分研究(II)[J].云南大学学报(自然科学版),2007,29(3):287~290.
[4]Li Q,Li B,Dong C Y,et al.20(S)-ginsenoside Rh2 suppresses proliferation and migration of hepatocellular carcinoma cells by targeting EZH2 to regulate CDKN2A-2B gene cluster transcription[J].European Journal of Pharmacology,2017,815(1):173~180.
[5]Li P,Zhou X Y,Qu D,et al.Preliminary study on fabrication,characterization and synergistic antilung cancer effects of self-assembled micelles of covalently conjugated celastrol-polyethylene glycol-ginsenoside Rh2[J].Drug delivery,2017,24(1):834~845.
[6]Kim W Y,Kim J M,Han S B,et al.Steaming of ginseng at high temperature enhances biological activity[J].J Nat Prod,2000,63(12):1702~1704.
[7]Cho S H,Chung K S,Choi J H,et al.Compound K,a metabolite of ginseng saponin,induces apoptosis via caspase-8-dependent pathway in HL-60human leukemia cells[J].BMC Cancer,2009,9(1):449.
[8]尹天翔,王燕燕.人参皂苷Rg3对人肝癌细胞增殖、迁移、黏附和凋亡的影响及其作用机制[J].基础医学与临床,2015,35(3):303~307.
[9]张味娜,徐逸,俞敏.人参皂苷Rg3温敏纳米粒制备及对肝癌细胞抑制作用研究[J].实用药物与临床,2017,20(11):1231~1235.
[10]Yuan Z G,Jiang H,Zhu X H,et al.Ginsenoside Rg3 promotes cytotoxicity of Paclitaxel through inhibiting NF-κB signaling and regulating Bax/Bcl-2 expression on triple-negative breast cancer[J].Biomedicine&Pharmacotherapy,2017,89(1):227~232.
[11]付玉,王超,尹成日.菌株CG2对三七总皂苷的微生物转化及其转化机理[J].延边大学农学学报,2011,33(2):117~121.
[12]武伦鹏,白龙律,韩春峰,等.微生物转化人参主要皂苷Rb1为C-K的研究[J].人参研究,2016,28(2):7~11.
[13]Fu Y,Yin Z H,Song X L,Yin C R.Diversity of cultivableβ-glycosidase-producing microorganisms isolated from the soil of a ginseng field and their ginsenosides-hydrolyzing activity[J].Letters in Applied Microbiology,2013,58(2):138~144.
[14]Fu Y,Yin Z H,Wu L P,Yin C R.Fermentation of ginseng extracts by Penicillium simplicissimum GS33 and anti-ovarian cancer activity of fermented products[J].World Journal of Microbiology&Biotechnology,2014,30(3):1019~1025.
[15]Fu Y,Yin Z H,Wu L P,Yin C R.Biotransformation of ginsenoside Rb1 to ginsenoside C-K by endophytic fungus Arthrinium sp.GE 17-18 isolated from Panax ginseng[J].Letters in Applied Microbiology,2016,63(3):196~201.
[16]Fu Y,Yin Z H,Yin C R.Biotransformation of ginsenoside Rb1 to ginsenoside Rg3 by endophytic bacterium Burkholderia sp.GE 17-7 isolated from Panax ginseng[J].Journal of Applied Microbiology,2017,122(6):1579~1585.
[17]赵林果,孟鹏,李丽娟,余世袁.利用七叶灵显色技术检验和判断β-葡萄糖苷酶的研究[J].食品与发酵工业,2008,34(12):163~166.
[18]包名家,周维民,孟庆敏.纸片法快速细菌七叶灵水解试验的研究[J].职业与健康,2003,19(10):67~69.