盐酸水解人参总皂苷制备20(R)-人参皂苷Rg
|
摘要
目的本文主要利用酸催化水解人参总皂苷制备20(R)-人参皂苷Rg3。采用大孔树脂吸附法对人参总皂苷进行脱糖,硅胶柱层析法和制备HPLC法分离纯化单体人参皂苷,UPLC法能够快速、灵敏度高检测人参皂苷含量。结论研究表明,人参总皂苷在高温、低pH值条件下,20(R)-人参皂苷Rg3的转化率较高。该方法成本低廉,操作简单,适合工业化生产,为20(R)-人参皂苷Rg3在医药行业中的应用提供了基础理论研究。
Objective Ginseng total saponin was transformed to 20(R)-ginsenoside Rg3 with acid hydrolysis.Ginseng total saponingot rid of the sugar by macroporous resin adsorption method,monomer ginsenoside was isolated by silica gel column chromatography and purified by preparative HPLC.Ultra Performance Liquid Chromatography has a good resolution and sensitivity to check ginsenosides.Results showed 20(R)-ginsenoside Rg3 could be transformed from PPD type of ginseng saponin under high temperature and low pH value conditions.Acid hydrolysis is a low-cost,easy operation and designed for industrialization.
Objective Ginseng total saponin was transformed to 20(R)-ginsenoside Rg3 with acid hydrolysis.Ginseng total saponingot rid of the sugar by macroporous resin adsorption method,monomer ginsenoside was isolated by silica gel column chromatography and purified by preparative HPLC.Ultra Performance Liquid Chromatography has a good resolution and sensitivity to check ginsenosides.Results showed 20(R)-ginsenoside Rg3 could be transformed from PPD type of ginseng saponin under high temperature and low pH value conditions.Acid hydrolysis is a low-cost,easy operation and designed for industrialization.
引文
[1]张均田.人参研究的最新进展[J].江苏大学学报(医学版),2009,19(3):185~191.
[2]国家中医药管理局《中华本草》编委会.《中华本草》[M].上海科学技术出版社,1999.
[3]王铁生.中国人参[M].天津:天津科学技术出版社,2001,87~125.
[4]Kim,S.,Y.Lee,J.Park,and S.Lee.GinsenosideRs4,a new type of ginseng saponin concurrently induces apoptosis and selectively elevates protein levels of p53and p21WAF1 in human hepotoma SK-HEP-1 cells.Eur J Cancer.1999.35:507~511.
[5]陶厚全,姚明,邹寿椿,等.血管生成抑制剂Rg3对胃癌生长和转移抑制作用的研究[J].中华外科杂志,2002,40(8):602~608.
[6]辛颖,倪劲松,王新蕊,等.20(S)-人参皂苷Rg3抗B16黑色素瘤转移的作用[J].吉林大学学报:医学版,2004,30(4):540~542.
[7]张晶,王世荣,陈全成,等.人参皂苷Rg3(R),人参皂苷Rg3(S)、Rg5/Rk1对乙醇致小鼠记忆阻碍改善作用的影响[J].吉林农业大学学报,2006,28(3):293~295.
[8]王庭富,孟正木.人参皂苷Rg3对免疫功能的影响[J].中国药科大学学报,1999,30(2):133~135.
[9]Cho,W.,W.Chung,S.Lee,A.Leung,C.Cheng,and K.Yue.2006.Ginsenoside Re of Panax ginseng possesses significant antioxidant and anti-hyperlipidemic efficacies in streptozotocin-induced diabetic rats.Eur.J.Pharmacol.550:173~179.
[10]Cheng L Q,Na J R,Bang M H,et al.Conversion of major ginsenoside Rb1 to 20(S)-ginsenoside Rg3 by Microbacterium sp.GS514[J].Phytochemistry,2008,69:218~224.
[11]Yan Q,Zhou W,Li X E,et al.Purification method improvement and characterization of a novel insenosidehydrolyzingβ-glucosidase from Paecilomyces Bainier sp.229[J].Biosci Biotechnol Biochem,2008,72(2):352~359.
[12]白龙律,臧蕴霞,尹成日.微生物转化人参皂苷Rb1为Rg3的研究[J].延边大学学报,2009,35(2):141~144.
[13]金凤燮,鱼红闪,张春枝,等.高活性红参的制作方法:中国,CN1846720A[P],2006~10~18.
[14]杨崇仁,倪凤仪,周俊,等.三七中达玛烷型皂甙的热不稳定性及酸水解产物[J].云南植物研究,1986,8(1):87~92.
[2]国家中医药管理局《中华本草》编委会.《中华本草》[M].上海科学技术出版社,1999.
[3]王铁生.中国人参[M].天津:天津科学技术出版社,2001,87~125.
[4]Kim,S.,Y.Lee,J.Park,and S.Lee.GinsenosideRs4,a new type of ginseng saponin concurrently induces apoptosis and selectively elevates protein levels of p53and p21WAF1 in human hepotoma SK-HEP-1 cells.Eur J Cancer.1999.35:507~511.
[5]陶厚全,姚明,邹寿椿,等.血管生成抑制剂Rg3对胃癌生长和转移抑制作用的研究[J].中华外科杂志,2002,40(8):602~608.
[6]辛颖,倪劲松,王新蕊,等.20(S)-人参皂苷Rg3抗B16黑色素瘤转移的作用[J].吉林大学学报:医学版,2004,30(4):540~542.
[7]张晶,王世荣,陈全成,等.人参皂苷Rg3(R),人参皂苷Rg3(S)、Rg5/Rk1对乙醇致小鼠记忆阻碍改善作用的影响[J].吉林农业大学学报,2006,28(3):293~295.
[8]王庭富,孟正木.人参皂苷Rg3对免疫功能的影响[J].中国药科大学学报,1999,30(2):133~135.
[9]Cho,W.,W.Chung,S.Lee,A.Leung,C.Cheng,and K.Yue.2006.Ginsenoside Re of Panax ginseng possesses significant antioxidant and anti-hyperlipidemic efficacies in streptozotocin-induced diabetic rats.Eur.J.Pharmacol.550:173~179.
[10]Cheng L Q,Na J R,Bang M H,et al.Conversion of major ginsenoside Rb1 to 20(S)-ginsenoside Rg3 by Microbacterium sp.GS514[J].Phytochemistry,2008,69:218~224.
[11]Yan Q,Zhou W,Li X E,et al.Purification method improvement and characterization of a novel insenosidehydrolyzingβ-glucosidase from Paecilomyces Bainier sp.229[J].Biosci Biotechnol Biochem,2008,72(2):352~359.
[12]白龙律,臧蕴霞,尹成日.微生物转化人参皂苷Rb1为Rg3的研究[J].延边大学学报,2009,35(2):141~144.
[13]金凤燮,鱼红闪,张春枝,等.高活性红参的制作方法:中国,CN1846720A[P],2006~10~18.
[14]杨崇仁,倪凤仪,周俊,等.三七中达玛烷型皂甙的热不稳定性及酸水解产物[J].云南植物研究,1986,8(1):87~92.