高温胁迫对野生湖北百合生理指标的影响
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摘要
为了解贵州野生湖北百合的耐热性,对其设置不同高温(28℃、31℃、34℃、37℃、41℃、43℃)和不同时间(12、24、48、72h)处理,测定各处理对叶片的膜透性、抗氧化物系统保护酶、可溶性物质等生理指标。结果表明,随着温度升高和时间的延长,MDA含量上升、相对电导率增加,则膜透性增强;可溶性糖含量、Pro含量呈现升高趋势;CAT活性呈现先升高后下降趋势。同一温度下,随着时间的延长,各处理POD活性呈现先升高后下降趋势,但43℃处理呈持续升高;31℃、34℃和37℃处理不同时间SOD活性呈现下降-升高-下降趋势变化,28℃、40℃和43℃处理呈现先降低后升高趋势;可溶性蛋白质含量呈升高-降低-升高的变化趋势。通过主成分分析,MDA含量、POD活性、Pro含量和可溶性糖含量是野生湖北百合高温胁迫的主导指标;其次,SOD活性、可溶性蛋白含量也是次要的指标。
In order to understand the heat resistance of wild Lilium henryi occurring in Guizhou Province,wild L.henryi plants were treated by different temperatures(namely,28℃,31℃,34℃,37℃,41℃ and43℃)with different time durations(12,24,48 and 72h).Relative physiological indices of the leaves were measured,including membrane permeability,protective enzymes of anti-oxidation system and soluble substances.The results showed that the MDA content and relative electric conductivity,membrane permeability,soluble sugar and proline contents of the leaves increased with the increase of temperature and time.CAT activity tended to increase first and then decrease.Under the same temperature,POD activity of all treatments increased first and then decreased with time,except for the 43℃treatment,in which POD activity increased all the time.Under the temperatures of 31℃,34℃ and 37℃,SOD activity showed a trend of decrease-increase-decrease,while under the temperatures of 28℃,40℃and 43℃,SOD activity tended to decrease and then increase.Soluble protein content showed a trend of increase-decrease-increase.By means of principal analysis,POD activity,MDA,proline and soluble sugar content were the main physiological indexes under high temperature stress,followed by SOD activity and soluble protein content.
In order to understand the heat resistance of wild Lilium henryi occurring in Guizhou Province,wild L.henryi plants were treated by different temperatures(namely,28℃,31℃,34℃,37℃,41℃ and43℃)with different time durations(12,24,48 and 72h).Relative physiological indices of the leaves were measured,including membrane permeability,protective enzymes of anti-oxidation system and soluble substances.The results showed that the MDA content and relative electric conductivity,membrane permeability,soluble sugar and proline contents of the leaves increased with the increase of temperature and time.CAT activity tended to increase first and then decrease.Under the same temperature,POD activity of all treatments increased first and then decreased with time,except for the 43℃treatment,in which POD activity increased all the time.Under the temperatures of 31℃,34℃ and 37℃,SOD activity showed a trend of decrease-increase-decrease,while under the temperatures of 28℃,40℃and 43℃,SOD activity tended to decrease and then increase.Soluble protein content showed a trend of increase-decrease-increase.By means of principal analysis,POD activity,MDA,proline and soluble sugar content were the main physiological indexes under high temperature stress,followed by SOD activity and soluble protein content.
引文
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[2]游立刚.百合的功效与作用[J].吉林蔬菜,2015(10):33.
[3]包满珠.花卉学[M].北京:中国农业出版社,2005.
[4] ZHOU J,WANG J,YU J Q,et al.Role and regulation of autophagy in heat stress responses of tomato plants[J].Frontier in Plant Sci.,2014,5:174.
[5]刘军铭,赵琪,尹赜鹏,等.利用蛋白质组学技术揭示的植物高温胁迫响应机制[J].应用生态学报,2015,26(8):2561-2570.LIU J M,ZHAO Q,YIN Z P,et al.Heat-responsive mechanisms in plants revealed by proteomic analysis:a review[J].Chinese Journal of Applied Ecology,2015,26(8):2561-2570.(in Chinese)
[6]杨炜茹,张启翔,孙明,等.高温胁迫对岷江百合幼苗耐热指数和理化特性的影响[J].华南农业大学学报,2010,31(1):51-54.
[7]刘艳妮,佘奎军,王飞,等.高温胁迫对铁炮百合生理特性的影响[J].宁夏农林科技,2012,53(12):79-80.
[8]吴在生,曹晓娟,张智,等.高温胁迫下东方百合‘Sorbonne’形态及生理响应[J].西北农业学报,2011,20(2):174-177.
[9]程千钉,刘桂芳,杨利平,等.百合新种质苗期耐热性比较[J].河北农业大学学报,2012,35(5):34-39.
[10]刘雪凝.高温胁迫对百合抗热性的影响[D].保定:河北农业大学,2010.
[11]欧阳文.北方温室亚洲百合东方百合双茬优质高效栽培模式[J].吉林蔬菜,2016(6):44-45.
[12]吴运荣.百合种植技术[J].农技服务,2016,33(4):73.
[13]杨苓莉,唐欢.川东北地区食用百合栽培技术要点[J].南方农业,2015,9(1):7-8.
[14]向地英,张钢,杨利平,等.热激诱导对高温胁迫下百合相对电导率及电阻抗参数的影响[J].西北农业学报,2014,23(9):189-195.
[15]朱志国,季晓莲.百合鳞茎形成的生理生化研究[J].中国农学通报,2015,31(10):138-141.ZHU Z G,JI X L.Physiological and biochemical research on lily bulb formation[J].Chinese Agricultural Science Bulletin,2015,31(10):138-141.(in Chinese)
[16]王玲丽,贾文杰,马璐琳,等.低温胁迫对不同百合主要生理指标的影响[J].植物生理学报,2014,50(9):1413-1422.
[17]张志良,瞿伟箐,李小芳,等.植物生理学[M].北京:高等教育出版社,2009:103-104,125-126.
[18]邢晓蕾,刘冬云,胡松竹,等.高温胁迫对兰州百合幼苗生理指标的影响[J].河北农业大学学报,2010,33(5):65-68.
[19]杜凌,吴楠,董万鹏,等.高温胁迫对淡黄花百合幼苗生理指标的影响[J].种子,2016,35(11):76-78.
[20]张志伟,周津吟,李莎,等.高温胁迫对胭脂花叶片细胞膜透性影响研究[J].安徽农学通报,2015,21(3/4):33-34.
[21]黄增艳,冷寒冰,蒋昌华.热激对荚蒾品种部分生理指标的影响研究[J].西北林学院学报,2017,32(2):97-100.HUANG Z Y,LENG H B,JIANG C H.Effects of high temperature on physiological indexes of Viburnum Species[J].Journal of Northwest Forestry University,2017,32(2):97-100.(in Chinese)
[22]黄滔,刘玮,唐红,等.4种优良观赏竹耐高温与抗旱性研究及其综合评价[J].西北林学院学报,2014,29(4):26-32.HUANG T,LIU W,TANG H,et al.High temperature and drought resisances of 4excellent ornamental bamboo[J].Journal of Northwest Forestry University,2014,29(4):26-32.(in Chinese)
[23]贾文杰,王祥宁,马璐琳,等.干旱胁迫对东方百合根叶生理功能及花粉育性的影响[J].西北植物学报,2017,37(11):2194-2203.JIA W J,WANG X N,MA L L,et al.Effect of water stress on root,leaf function and pollen fertility of cut flower oreiental lily[J].Acta Bot.Boreal-Occident.Sin.,2017,37(11):2194-2203.(in Chinese)
[24]张哲,阂红梅,夏关均,等.高温胁迫对植物生理影响研究进展[J].安徽农业科学,2010,38(16):8338-8339.
[25]杨利平,李蕊.百合亲本及其杂种幼苗对高温逆境的响应[J].东北林业大学学报,2012,40(12):63-66.
[26]申惠翡,赵冰.杜鹃花品种耐热性评价及其生理机制研究[J].植物生理学报,2018,54(2):335-345.SHEN H F,ZHAO B.Study on evaluation of heat tolerance and its physiological mechanisms in Rhododendroncultivars[J].Plant Physiology Journal,2018,54(2):335-345.(in Chinese)
[27]周广.高温胁迫对7种杜鹃生理生化特性的影响[D].南昌:南昌大学,2010.
[28] HABA P D,MATA L D,MOLINA E,et al.High temperature promotes early senescence in primary leaves of sunflower(Helianthus annuus L.)plants[J].Can.J.Plant Sci.,2014,94:659-668.