高温胁迫下丛枝菌根真菌对百合耐热性的影响
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摘要
本试验于温室容器栽培条件下,研究了昼/夜温度(25℃/15℃、35℃/25℃、45℃/35℃)和接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)摩西斗管囊霉(Funneliformis mosseae)和/或变形球囊霉(Glomus versiforme)对百合(Lilium brownii)东方百合品种‘西伯利亚’(Siberia)耐热性的影响。结果表明,同时双接种摩西斗管囊霉和变形球囊霉(混合接种)的根系侵染率、丛枝着生率和泡囊数最高,该处理的百合株高、茎粗、花蕾数、地上部干重、地下部干重及叶面积均显著高于不接种对照;接种AMF促进百合的生长效应大小顺序:混合菌种﹥变形球囊霉﹥摩西斗管囊霉。于35℃/25℃温度下处理48h,与对照相比,混合接种的百合叶片可溶性蛋白和脯氨酸含量分别提高15%和14%,丙二醛含量和相对电导率显著低于不接种对照。接种变形球囊霉和接种混合菌种可以降低高温对百合植株的伤害程度,而接种摩西斗管囊霉改善百合耐高温效用不显著。结论认为接种AMF能在一定范围和程度上提高百合的耐热性,其中以摩西斗管囊霉和变形球囊霉混合接种效应最大。
Effects of arbuscular mycorrhizal fungus(AMF)Glomus versiforme,Funneliformis mosseae,and G.versiforme+ F.mosseae on the heat tolerence of Lilium brownii(cultivar Siberia)under the different daily/night temperatures(25 ℃/15 ℃,35 ℃/25 ℃,45 ℃/35 ℃)were investigated in a greenhouse.Results showed that the mixed inoculation with G.versiforme+ F.mosseae gave the best mycorrhizal colonization status,with the highest arbuscule rate and vesicle numbers;and plant height,stem diameter,number of buds,shoot dry weight,root dry weight and leaf area of this treated lily were greater than that of the non-inoculation control.The order of growth effect of Lilium brownii inoculated with AMF was the mixed strain>G.versiforme> F.mosseae.Being compared with the control,the contents of soluble protein and proline in leaves of lily inoculated with mixed AMF inoculation increased by 15%and14%,respectively,and contents of malondialdehyde content and relative conductivity were significantly lower than those of the control.The mixed inoculation and the single inoculation with G.versiforme could reduce the injury degree of high temperature to lily plants.But it was not significant in improving heat tolerence of lily by the single inoculation with F.mosseae.It was concluded that the inoculation of AMF could improve heat tolerance of lily and the mixed inoculation with G.versiforme and F.mosseae showed superior benefits.
Effects of arbuscular mycorrhizal fungus(AMF)Glomus versiforme,Funneliformis mosseae,and G.versiforme+ F.mosseae on the heat tolerence of Lilium brownii(cultivar Siberia)under the different daily/night temperatures(25 ℃/15 ℃,35 ℃/25 ℃,45 ℃/35 ℃)were investigated in a greenhouse.Results showed that the mixed inoculation with G.versiforme+ F.mosseae gave the best mycorrhizal colonization status,with the highest arbuscule rate and vesicle numbers;and plant height,stem diameter,number of buds,shoot dry weight,root dry weight and leaf area of this treated lily were greater than that of the non-inoculation control.The order of growth effect of Lilium brownii inoculated with AMF was the mixed strain>G.versiforme> F.mosseae.Being compared with the control,the contents of soluble protein and proline in leaves of lily inoculated with mixed AMF inoculation increased by 15%and14%,respectively,and contents of malondialdehyde content and relative conductivity were significantly lower than those of the control.The mixed inoculation and the single inoculation with G.versiforme could reduce the injury degree of high temperature to lily plants.But it was not significant in improving heat tolerence of lily by the single inoculation with F.mosseae.It was concluded that the inoculation of AMF could improve heat tolerance of lily and the mixed inoculation with G.versiforme and F.mosseae showed superior benefits.
引文
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[22]孔钰凤,朱先灿,张建峰,田春杰.野生大豆与栽培大豆抗旱性对接种丛枝菌根真菌的响应[J].土壤与作物,2017,6(01):25-31
[23]肖质净.丛枝菌根真菌(AMF)对植物生化变化影响研究进展[J].农业科技与装备,2017(05):11-12
[24]Mirzaee M,Moieni A,Ghanati F.Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola(Brassica napus)cultivars[J].Journal of Agricultural Science&Technology,2013,15(3):593-602
[25]李小玲,雒玲玲,华智锐.高温胁迫下高山杜鹃的生理生化响应[J].西北农业学报,2018,27(02):253-259
[26]尹慧,陈秋明,何秀丽,等.短暂高温对百合植株抗氧化酶系统的影响[J].园艺学报,2007,34(2):509-512
[27]孔静,裴宗平,杜旼,等.水分胁迫下AM真菌对紫花苜蓿生长及抗旱性的影响[J].北方园艺,2014(09):179-182
[28]费昭雪,刘莉丽,胡蝶,等.高温胁迫对西洋杜鹃理化指标的影响[J].北方园艺,2018(08):102-105
[2]刘润进,唐明,陈应龙.菌根真菌与植物抗逆性研究进展[J].菌物研究,2017,15(01):70-88
[3]王维华,孙丹丹,郑锦龙,等.AM真菌与作物秸秆对土壤养分和植物生长的影响[J].青岛农业大学学报(自然科学版),2018,35(02):83-89
[4]孙鹏飞,郭绍霞.AM真菌对薰衣草光合作用和生长的影响[J].青岛农业大学学报(自然科学版),2017,34(04):235-239
[5]李文彬,郭绍霞.AM真菌和PGPR对多环芳烃污染土壤中的高羊茅生理和生长的影响[J].青岛农业大学学报(自然科学版),2017,34(04):240-248
[6]Zhu X C,Song F B,Xu H W.Influence of arbuscular mycorrhiza on lipid peroxidation and antioxidant enzyme activity of maize plants under temperature stress[J].Mycorrhiza,2010,20:325-332
[7]Mathur S,Sharma M P,Jajoo A.Improved photosynthetic efficacy of maize(Zea mays)plants with arbuscular mycorrhizal fungi(AMF)under high temperature stress[J].Journal of Photochemistry and Photobiology B-Biology,2018,180:149-15
[8]曾斌,孙中海,何科佳,等.AMF对不同温度下枳实生苗抗氧化系统的影响[J].湖南农业科学,2012(21):117-120
[9]张霁,刘大会,郭兰萍,等.不同温度下丛枝菌根对苍术根茎生物量和挥发油的影响[J].中草药,2011,42(02):372-375
[10]马通,刘润进,李敏.丛枝菌根真菌对生菜耐热性的效应[J].植物生理学报,2015,51(11):1919-1926
[11]丁芳兵,孙伟博,原雅玲,等.秦巴山区百合种质资源及开发利用[J].湖北农业科学,2017,56(15):287
[12]李国庆.百合耐热相关基因LlbZIP1的克隆及功能分析[A].中国园艺学会.中国园艺学会2017年论文摘要集[C].中国园艺学会:中国园艺学会,2017:1
[13]邢景景.淡黄花百合的引种栽培及耐热性机理探究[D].武汉:华中农业大学,2017
[14]刘润进,陈应龙.菌根学[M].北京:科学出版社,2007,1-447
[15]贾开志,陈贵林.高温胁迫下不同茄子品种幼苗耐热性研究[J].生态学杂志,2005,24(4):398-401
[16]王学奎.植物生理生化试验原理和技术[M].北京:高等教育出版社,2006
[17]孟焕文,张彦峰,程智慧,等.黄瓜幼苗对热胁迫的生理反应及耐热鉴定指标筛选[J].西北农业学报,2000,9(l):96-99
[18]王丽媛,丁国华,黎莉.脯氨酸代谢的研究进展[J].哈尔滨师范大学自然科学学报,2010,26(2):84-89
[19]刘春风,张往祥,孙垒,等.高温对观赏海棠生长和光合作用的影响[J].南京林业大学学报:自然科学版,2013,37(4):17-22
[20]邢晓蕾,刘冬云,胡松竹.高温胁迫对兰州百合幼苗生理指标的影响[J].河北农业大学学报,2010,33(05):65-68
[21]韩玮,孙晨曦,赵和丽,等.高温胁迫后小白菜的补偿生长能力及防御机制[J].中国农业气象,2018,39(02):119-128
[22]孔钰凤,朱先灿,张建峰,田春杰.野生大豆与栽培大豆抗旱性对接种丛枝菌根真菌的响应[J].土壤与作物,2017,6(01):25-31
[23]肖质净.丛枝菌根真菌(AMF)对植物生化变化影响研究进展[J].农业科技与装备,2017(05):11-12
[24]Mirzaee M,Moieni A,Ghanati F.Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola(Brassica napus)cultivars[J].Journal of Agricultural Science&Technology,2013,15(3):593-602
[25]李小玲,雒玲玲,华智锐.高温胁迫下高山杜鹃的生理生化响应[J].西北农业学报,2018,27(02):253-259
[26]尹慧,陈秋明,何秀丽,等.短暂高温对百合植株抗氧化酶系统的影响[J].园艺学报,2007,34(2):509-512
[27]孔静,裴宗平,杜旼,等.水分胁迫下AM真菌对紫花苜蓿生长及抗旱性的影响[J].北方园艺,2014(09):179-182
[28]费昭雪,刘莉丽,胡蝶,等.高温胁迫对西洋杜鹃理化指标的影响[J].北方园艺,2018(08):102-105