紫草素在玻碳电极上的电化学行为及应用
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摘要
采用电化学测试方法测定了紫草素在玻碳电极上的电化学行为。结果表明,紫草素在玻碳电极产生一对氧化还原波,在pH为3.98及HAc-NaAc(20%乙醇)缓冲溶液中。证实该反应是以吸附控制为主的电极过程。同时,以氧化峰为对象的循环伏安法研究了峰电流与药物浓度的关系。峰值电流与紫草素浓度在2.08×10~(-8)～1.82×10~(-6)mol·L~(-1)范围内具有良好的线性关系。该方法可用于中草药紫草中紫草素及其衍生物总含量的测定,样品无需预处理即可直接测定。通过方波伏安法测出新疆特有紫草素的平均含量为9.063m·mg~(-1),相对标准偏差3.08%,证明采用此方法测定紫草素含量是可行的。
The electrochemical behavior of the shikonin on the glassy carbon electrode was tested by electrochemical method.The results showed that in the HAc-NaAc(20%ethanol)buffer solution with pH 3.98,the shikonin produced a pair of redox waves at the glassy carbon electrode,and confirmed that the reaction was an electrode process dominated by adsorption control.At the same time,the relationship between peak current and drug concentration in was investigated bycyclic voltammetry,and the result showed that the peak current and the concentration in 2.08 × 10~(-8)～1.82 × 10~(-6)mol·L~(-1) have a better linear relationship.This method can be used to determine the total content of shikonin and its derivatives in Chinese herbal medicine,and the sample can be directly determined without pretreatment and spearation.The average content of Xinjiang shikonin is 9.063 m·mg~(-1) by cyclic voltammetry,and the relative standed devation is 3.08%,which proves that it is feasible to use this method to determine the content of the shikonin.
The electrochemical behavior of the shikonin on the glassy carbon electrode was tested by electrochemical method.The results showed that in the HAc-NaAc(20%ethanol)buffer solution with pH 3.98,the shikonin produced a pair of redox waves at the glassy carbon electrode,and confirmed that the reaction was an electrode process dominated by adsorption control.At the same time,the relationship between peak current and drug concentration in was investigated bycyclic voltammetry,and the result showed that the peak current and the concentration in 2.08 × 10~(-8)～1.82 × 10~(-6)mol·L~(-1) have a better linear relationship.This method can be used to determine the total content of shikonin and its derivatives in Chinese herbal medicine,and the sample can be directly determined without pretreatment and spearation.The average content of Xinjiang shikonin is 9.063 m·mg~(-1) by cyclic voltammetry,and the relative standed devation is 3.08%,which proves that it is feasible to use this method to determine the content of the shikonin.
引文
[1]李亮,陈钰沁,张超,等.紫草素的研究进展[J].云南中医学院学报,2011,34(5):321-324.
[2]Shahla Karim Baloch.A potent anticancer agent of shikonin derivative targeting tublin[J].Chirality,2015,27(3):193-198.
[3]傅善林.新疆软紫草中萘醌色素的研究[J].中草药,1986,17(10):2-5.
[4]徐飞,于慧,吴启南.电化学技术在中药质量控制方面的研究进展[J].分析试验室,2011,34(4):50-52.
[5]刘芳,伍伟祯.薄层扫描法测定烧伤膏中左旋紫草素的含量[J].基层中药杂志,2001,15(2):21-22.
[6]郭红军,简丽娜,孔焕宇.高效液相色谱法测定玉红膏中左旋紫草素的含量[J].时珍国医国药,2006,17(4):489-490.
[7]刘玉梅.高效液相色谱法同时测定紫草素及其衍生物[J].中国酿造,2011(1):173-176.
[8]昝珂,郑凰雅,刘杰,等.HPLC测定新疆紫草不同部位中8种羟基萘醌的含量[J].中国中药杂志,2017,42(13):2532-2537.
[9]孙健文,温宪春,蔡德富,等.RP-HPLC法同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素的含量[J].中国药房,2018,29(4):455-457.
[10]白研,毋福海,罗碧莲,等.高效毛细管电泳法测定新疆紫草中左旋紫草素的含量[J].中国药房,2010,21(19):1790-1791.
[11]申刚义,于婉婷,张爱芹,等.毛细管电泳分离法检测新疆紫草中萘醌类成分[J].分析试验室,2014,33(5):513-515.
[12]刘玉梅.紫草中左旋紫草素的分光光度定量方法研究[J].药物分析杂志,2011,31(6):1141-1145.
[13]张金磊,黄广军,兰翠玲,等.新铜试剂分光光度法测定片剂中的对乙酰氨基酚含量及其反应机理的研究[J].化学研究与应用,2015(10):1518-1522.
[14]周红碧,李乐翔等.方波伏安法测定紫草素及其电化学行为的考察[J].分析测试技术与仪器,2011,17(3):160-163.
[15]轩卫华,钱宗耀,李炳奇,等.紫草素的电化学伏安行为研究[J].分析试验室,2010,29(2):101-103.
[16]潘志斌,周学酬.对乙酰氨基酚在单壁碳纳米管/聚甘氨酸复合膜修饰玻碳电极上的电化学行为及测定[J].化学研究与应用,2017,29(3):330-334.
[17]刘红,轩卫华,曹红,等.线性扫描伏安法测定丹参酮IIA[J].理化检测:化学分册,2011,l7(10):1129-1131.
[18]殷迎春,施桂英,李江,等.甘草苷在玻碳电极上的电化学行为及其测定[J].分析测试技术与仪器,2012,18(2):2205-2207.
[2]Shahla Karim Baloch.A potent anticancer agent of shikonin derivative targeting tublin[J].Chirality,2015,27(3):193-198.
[3]傅善林.新疆软紫草中萘醌色素的研究[J].中草药,1986,17(10):2-5.
[4]徐飞,于慧,吴启南.电化学技术在中药质量控制方面的研究进展[J].分析试验室,2011,34(4):50-52.
[5]刘芳,伍伟祯.薄层扫描法测定烧伤膏中左旋紫草素的含量[J].基层中药杂志,2001,15(2):21-22.
[6]郭红军,简丽娜,孔焕宇.高效液相色谱法测定玉红膏中左旋紫草素的含量[J].时珍国医国药,2006,17(4):489-490.
[7]刘玉梅.高效液相色谱法同时测定紫草素及其衍生物[J].中国酿造,2011(1):173-176.
[8]昝珂,郑凰雅,刘杰,等.HPLC测定新疆紫草不同部位中8种羟基萘醌的含量[J].中国中药杂志,2017,42(13):2532-2537.
[9]孙健文,温宪春,蔡德富,等.RP-HPLC法同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素的含量[J].中国药房,2018,29(4):455-457.
[10]白研,毋福海,罗碧莲,等.高效毛细管电泳法测定新疆紫草中左旋紫草素的含量[J].中国药房,2010,21(19):1790-1791.
[11]申刚义,于婉婷,张爱芹,等.毛细管电泳分离法检测新疆紫草中萘醌类成分[J].分析试验室,2014,33(5):513-515.
[12]刘玉梅.紫草中左旋紫草素的分光光度定量方法研究[J].药物分析杂志,2011,31(6):1141-1145.
[13]张金磊,黄广军,兰翠玲,等.新铜试剂分光光度法测定片剂中的对乙酰氨基酚含量及其反应机理的研究[J].化学研究与应用,2015(10):1518-1522.
[14]周红碧,李乐翔等.方波伏安法测定紫草素及其电化学行为的考察[J].分析测试技术与仪器,2011,17(3):160-163.
[15]轩卫华,钱宗耀,李炳奇,等.紫草素的电化学伏安行为研究[J].分析试验室,2010,29(2):101-103.
[16]潘志斌,周学酬.对乙酰氨基酚在单壁碳纳米管/聚甘氨酸复合膜修饰玻碳电极上的电化学行为及测定[J].化学研究与应用,2017,29(3):330-334.
[17]刘红,轩卫华,曹红,等.线性扫描伏安法测定丹参酮IIA[J].理化检测:化学分册,2011,l7(10):1129-1131.
[18]殷迎春,施桂英,李江,等.甘草苷在玻碳电极上的电化学行为及其测定[J].分析测试技术与仪器,2012,18(2):2205-2207.