壳寡糖在糖尿病皮肤伤口愈合中的应用及研究进展
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摘要
糖尿病慢性皮肤伤口的愈合,是临床治疗中的难点和基础研究中的热点。传统治疗方法效果并不理想,而组织工程技术的发展,为其治疗提供了新途径。现今已有将壳寡糖作为伤口敷料的研究,并引起越来越多的关注,但关于壳寡糖用于糖尿病难愈性创面的研究并不多。本篇综述将概述壳寡糖促进伤口愈合的生物学机制及特点,以及糖尿病皮肤创面的病理学改变,总结壳寡糖在糖尿病皮肤伤口愈合中的研究进展。
The chronic diabetic cutaneous wound healing is the problem of clinical practice and hot point of medical research. The effect of the current wound management is uncertain. It is fortunate that some new approaches and materials such as chitosan oligosaccride are approved along with the evolution of tissue regeneration and biotechnique. The results of chitosan oligosaccride applied on the wound dressing have been reported already and attracted more and more researchers' attentions. But there are not many research on the chitosan oligosaccride and chronic dibetic wound healinng. In this review,the biological mechanism of chitosan oligosaccride promotion on the wound healing is introduced briefly at first. Secondly,the pathology of diabetic skin wound is described. At last,the research and progress of chitosan oligosaccride on diabetic wound healing are summed up.
The chronic diabetic cutaneous wound healing is the problem of clinical practice and hot point of medical research. The effect of the current wound management is uncertain. It is fortunate that some new approaches and materials such as chitosan oligosaccride are approved along with the evolution of tissue regeneration and biotechnique. The results of chitosan oligosaccride applied on the wound dressing have been reported already and attracted more and more researchers' attentions. But there are not many research on the chitosan oligosaccride and chronic dibetic wound healinng. In this review,the biological mechanism of chitosan oligosaccride promotion on the wound healing is introduced briefly at first. Secondly,the pathology of diabetic skin wound is described. At last,the research and progress of chitosan oligosaccride on diabetic wound healing are summed up.
引文
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[6]刘红云,覃彩芹,李顺文,等.壳寡糖对急性酒精性肝损伤保护作用的研究[J].中国现代医学杂志,2010,20(3):350-353.
[7]许青松,麻攀,魏鹏,等.壳寡糖减弱乙醇对人胚胎肝细胞损伤的研究[J].中国生化药物杂志,2008,29(4):259-261.
[8]刘洋,韩宝芹,刘万顺,等.壳寡糖对酵母联合腺嘌呤致高尿酸血症小鼠的治疗作用[J].中国海洋大学学报(自然科学版),2009,39(S1):143-146.
[9]Pan H,Yang Q,Huang G,et al.Hypolipidemic effects of chitosan and its derivatives in hyperlipidemic rats induced by a high-fat diet[J].Food Nutr Res,2016,60:31137.
[10]Kumar SG,Rahman MA,Lee SH,et al.Plasma proteome analysis for anti-obesity and anti-diabetic potentials of chitosan oligosaccharides in ob/ob mice[J].Proteomics,2009,9(8):2149-2162.
[11]Yan YL,Hu Y,Simpson DJ,et al.Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88[J].J Agric Food Chem,2017,65(25):5142-5150.
[12]钱建瑛,丁振中,朱玉霞,等.壳寡糖对大肠杆菌抑菌活性研究[J].工业微生物,2016,46(6):13-19.
[13]Lee BC,Kim MS,Choi SH,et al.In vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against Vibrio vulnificus[J].Int J Mol Med,2009,24(3):327-333.
[14]刘碧源,高仕瑛,李邦良,等.壳寡糖抗菌活性的实验研究[J].中国生化药物杂志,2003,24(2):73-75.
[15]李昱,许青松,彭强,等.壳寡糖抗炎作用研究进展[J].中国生化药物杂志,2012,33(3):326-328.
[16]Lu Y,Slomberg DL,Schoenfisch MH.Nitric oxide-releasing chitosan oligosaccharides as antibacterial agents[J].Biomaterials,2014,35(5):1716-1724.
[17]Tin S,Sakharkar KR,Lim CS,et al.Activity of Chitosans in combination with antibiotics in Pseudomonas aeruginosa[J].Int J Biol Sci,2009,5(2):153-160.
[18]Liu X,Xia W,Jiang Q,et al.Effect of kojic acid-graftedchitosan oligosaccharides as a novel antibacterial agent on cell membrane of gram-positive and gram-negative bacteria[J].J Biosci Bioeng,2015,120(3):335-339.
[19]hlknecht C,Tegl G,Beer B,et al.Cellobiose dehydrogenase and chitosan-based lysozyme responsive materials for antimicrobial wound treatment[J].Biotechvnol Bioeng,2017,114(2):416-422.
[20]Qiao Y,Bai XF,Du YG.Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress[J].Int Immunopharmacol,2011,11(1):121-127.
[21]乔莹,许青松,白雪芳,等.壳寡糖减弱内毒素对小鼠巨噬细胞的损伤作用[J].中国生化药物杂志,2011,32(2):136-138.
[22]Kim JH,Kim YS,Hwang JW,et al.Sulfated chitosan oligosaccharides suppress LPS-induced NO production via JNK and NF-κB inactivation[J].Molecules,2014,19(11):18232-18247.
[23]Liu HT,Huang P,Ma P,et al.Chitosan oligosaccharides suppress LPS-induced IL-8 expression in human unbilical vein endothelial cells through blockade of p38 and Akt protein kinases[J].Acta Pharmacologica Sin,2011,32(4):478-486.
[24]Li Y,Xu Q,Wei P,et al.Chitosan oligosaccharides downregulate the expression of E-selectin and ICAM-1 induced by LPS in endothelial cells by inhibiting MAP kinase signaling[J].Int J Mol Med,2014,33(2):392-400.
[25]李昱,许青松,魏鹏,等.壳寡糖抑制脂多糖诱导的PIECs表达IL-8和VCAM-1作用的初步研究[J].现代生物医学进展,2013,13(34):6601-6604.
[26]Yang JW,Tian G,Chen DW,et al.Involvement of PKA signalling in anti-inflammatory effects of chitosan oligosaccharides in IPEC-J2 porcine epithelial cells[J].J Anim Physiol Anim Nutr(Berl),2018,102(1):252-259.
[27]Kunanusornchai W,Witoonpanich B,Tawonsawatruk T,et al.Chitosan oligosaccharide suppresses synovial inflammation via AMPK activation:An in vitro and in vivo study[J].Pharmacol Res,2016,113(Pt A):458-467.
[28]Chung MJ,Park JK,Park YI.Anti-inflammatory effects of lowmolecular weight chitosan oligosaccharides in Ig E-antigen complex-stimulated RBL-2H3 cells and asthma model mice[J].Int Immunopharmacol,2012,12(2):453-459.
[29]Huang B,Xiao D,Tan B,et al.Chitosan Oligosaccharide Reduces Intestinal Inflammation That Involves Calcium-Sensing Receptor(Ca SR)Activation in Lipopolysaccharide(LPS)-Challenged Piglets[J].J Agric Food Chem,2016,64(1):245-252.
[30]刘颖,白娟,柳云恩,等.壳寡糖对大鼠颅脑冲击伤的保护作用[J].中华创伤杂志,2016,32(12):1130-1136.
[31]Park PJ,Je JY,Kim SK.Angiotensin I converting enzyme(ACE)inhibitory activity of hetero-chitooligosacchatides prepared from partially different deacetylated chitosans[J].J Agric Food Chem,2003,51(17):4930-4934.
[32]麻攀,刘洪涛,许青松,等.壳寡糖缓解甲萘醌诱导巨噬细胞损伤机制初探[J].中国生物工程杂志,2011,31(6):18-21.
[33]Je JY,Park PJ,Kim SK.Free radical scavenging properties of hetero-chitooloigosaccharides using an ESR spectroscopy[J].Food Chem Toxicol,2004,42(3):381-387.
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