左金丸对二甲基苯蒽/巴豆油诱导小鼠皮肤肿瘤的预防作用
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摘要
目的:观察左金丸对二甲基苯蒽/巴豆油诱导小鼠皮肤肿瘤的预防作用。方法:二甲基苯蒽/巴豆油外涂建立小鼠皮肤肿瘤模型,左金丸按人体等效剂量灌胃,记录皮肤成瘤时间和瘤数量,检测小鼠血清IL-1、IL-6、hs-CRP、TNF-α和皮肤组织E-Cadherin、N-Cadherin及PCNA。结果:模型组小鼠成瘤时间范围为6～10周,平均每只小鼠致瘤数为(16. 3±3. 6);,左金丸组小鼠成瘤时间范围为8～10周,平均每只小鼠致瘤数为(10. 2±2. 3)。与正常小鼠比较,模型组小鼠血清IL-1、IL-6、hs-CRP和TNF-α均明显增高,上皮-间充质转化(EMT)和增殖标志表达显著增强,左金丸对上述模型小鼠血清炎症指标及皮肤组织EMT和增殖均有显著降低作用。结论:左金丸对二甲基苯蒽/巴豆油诱导小鼠皮肤肿瘤有预防作用。
Objective: To observe the preventive action of Zuojinpiu on DMBA/Croton oil-induced skin tumor in mice.Methods: The mouse skin tumorigenic model was carried out by DMBA/Croton oil for external application,and Zuojinpiu at human equivalent dose was administrated. The skin tumorigenic time and tumor number were recorded,the serum levels of IL-1,IL-6,hs-CRP and TNF-α were examined,and the skin tumorigenic tissues were cut to analyze the expression of ECadherin,N-Cadherin and PCNA by immunohistochemical staining. Results: The tumorigenic time in model group was in the range of 6-10 weeks and the mean of tumor number per mouse was 16. 3±3. 6. The tumorigenic time in Zuojinpiu group was in the range of 8-10 weeks and the mean of tumor number per mouse was 10. 2±2. 3. Compared with normal mice,The serum levels of IL-1,IL-6,hs-CRP and TNF-α were increased obviously in model group,Epithelial mesenchymal transition( EMT)and proliferative marker expression were enhanced dramatically. The above increased indexes in model mice could be decreased obviously by zuojinpiu. Conclusion: Zuojinpiu has the preventive action on DMBA/Croton oil-induced skin tumor in mice.
Objective: To observe the preventive action of Zuojinpiu on DMBA/Croton oil-induced skin tumor in mice.Methods: The mouse skin tumorigenic model was carried out by DMBA/Croton oil for external application,and Zuojinpiu at human equivalent dose was administrated. The skin tumorigenic time and tumor number were recorded,the serum levels of IL-1,IL-6,hs-CRP and TNF-α were examined,and the skin tumorigenic tissues were cut to analyze the expression of ECadherin,N-Cadherin and PCNA by immunohistochemical staining. Results: The tumorigenic time in model group was in the range of 6-10 weeks and the mean of tumor number per mouse was 16. 3±3. 6. The tumorigenic time in Zuojinpiu group was in the range of 8-10 weeks and the mean of tumor number per mouse was 10. 2±2. 3. Compared with normal mice,The serum levels of IL-1,IL-6,hs-CRP and TNF-α were increased obviously in model group,Epithelial mesenchymal transition( EMT)and proliferative marker expression were enhanced dramatically. The above increased indexes in model mice could be decreased obviously by zuojinpiu. Conclusion: Zuojinpiu has the preventive action on DMBA/Croton oil-induced skin tumor in mice.
引文
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[12]田同德,岳立云,田同良,等.肿瘤炎症微环境与免疫的关系及中医药干预策略[J].中医杂志,2017,58(3):209-213.
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[14]黄晓芬,李娟,李金田,等.论《伤寒论》保胃气思想[J].陕西中医药大学学报,2016,39(1):112-113.
[2]尹抗抗,梁媛,李玲,等.左金丸对实验性应激性胃溃疡的保护作用及其机制[J].中国微生态学杂志,2015,27(2):151-155.
[3]叶乃菁,刘宣,李琦.左金丸及其活性成分抗胃癌的实验研究进展[J].中华中医药杂志,2014,29(4):1162-1164.
[4]杜佳,孙阳,周钱梅,等.左金丸药物血清对人乳腺癌MCF-7细胞周期和细胞凋亡的影响[J].时珍国医国药,2016,27(11):2604-2606.
[5]付晓伶,隋华,潘树芳,等.左金丸对人结肠癌多药耐药细胞的逆转作用[J].中华中医药杂志,2014,29(3):844-847.
[6]韦平,梁兵,亓翠玲,等. Slit2在化学诱导的小鼠皮肤癌形成过程中作用[J].临床与实验病理学杂志,2012,28(3):330-33
[7]贲艺雯,张海湃,苏永华,等.皮肤癌的中药外治法[J].中医外治杂志,2012,21(1):47-49.
[8]周宜强.攻毒、排毒、解毒在治疗恶性肿瘤中的运用[J].世界中医药,2007,2(4):200-203.
[9] SHEN R,KIM JJ,YAO M,et al. Development and evaluation ofvitamin E d-α-tocopheryl polyethylene glycol 1000 succinate-mixed polymeric phospholipid micelles of berberine as ananticancer nanopharmaceutical[J]. Int J Nanomedicine,2016,11(8):1687-700.
[10] CHEN TC,CHIEN CC,WU MS,et al. Evodiamine from Evodiarutaecarpa induces apoptosis via activation of JNK and PERK inhuman ovarian cancer cells[J]. Phytomedicine,2016,23(1):68-78.
[11]龚梦鹃,周祥羽,岳贺,等.左金丸血清药物化学研究[J].中国民族民间医药,2017,26(11):56-62.
[12]田同德,岳立云,田同良,等.肿瘤炎症微环境与免疫的关系及中医药干预策略[J].中医杂志,2017,58(3):209-213.
[13]殷晓丽,刘兆玉.上皮间质转化在肿瘤中的研究进展[J].医学综述,2017,23(12):2359-2363.
[14]黄晓芬,李娟,李金田,等.论《伤寒论》保胃气思想[J].陕西中医药大学学报,2016,39(1):112-113.