一种适用于皮肤荧光的成像检测系统探究
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摘要
目前皮肤影像手段包括共聚焦激光扫描显微镜、光学相干断层扫描成像技术、高频超声、皮肤镜、荧光显微镜、反射式共聚焦显微镜、皮肤太赫兹成像等成像手段。每种成像手段只能突出皮肤肿瘤的部分特征,如超声能突出肿瘤的解剖结构成像,却不能准确定位肿瘤边缘;荧光成像能突出肿瘤边缘信息,但无法探查肿瘤深度等细节信息。国内目前的研究很少涉及不同皮肤影像手段的交叉研究。因此,本课题希望探究一种预留超声探头整合接口的荧光成像系统,为后续整合超声探头,进一步实现多模态皮肤肿瘤成像系统做贡献。课题研究包括皮肤肿瘤荧光原理探究,针对皮肤荧光的相机参数设计,整合超声探头的相机结构设计等部分,以及皮肤肿瘤荧光图像验证实验。
At present, skin imaging methods include confocal laser scanning microscope, optical coherence tomography imaging technology, high frequency ultrasound, skin mirror, fluorescence microscope, reflective confocal microscope, skin terahertz imaging and other imaging methods. Each imaging method can only highlight some of the characteristics of the skin tumor, such as ultrasound can highlight the anatomical structure of the tumor, but cannot accurately locate the edge of the tumor; ?uorescence imaging can highlight the edge information of the tumor, but it is not possible to detect the depth of the tumor and other details.At present, the current research in China rarely involves the cross-study of different skin imaging methods. Therefore, this topic hopes to explore a kind of ?uorescence imaging system, which is reserved for ultrasonic interface, and contributes to the further integration of multi-modal skin tumor imaging system. The research includes the fluorescence character of skin tumor, the?uorescence image collection experiment of skin tumor, the camera parameters for skin ?uorescence, and the camera structure integrating ultrasonic probe.
At present, skin imaging methods include confocal laser scanning microscope, optical coherence tomography imaging technology, high frequency ultrasound, skin mirror, fluorescence microscope, reflective confocal microscope, skin terahertz imaging and other imaging methods. Each imaging method can only highlight some of the characteristics of the skin tumor, such as ultrasound can highlight the anatomical structure of the tumor, but cannot accurately locate the edge of the tumor; ?uorescence imaging can highlight the edge information of the tumor, but it is not possible to detect the depth of the tumor and other details.At present, the current research in China rarely involves the cross-study of different skin imaging methods. Therefore, this topic hopes to explore a kind of ?uorescence imaging system, which is reserved for ultrasonic interface, and contributes to the further integration of multi-modal skin tumor imaging system. The research includes the fluorescence character of skin tumor, the?uorescence image collection experiment of skin tumor, the camera parameters for skin ?uorescence, and the camera structure integrating ultrasonic probe.
引文
[1]刘华绪,葛华勇,张福仁.反射式共聚焦激光扫描显微镜的原理[J].中国麻风皮肤病杂志,2010,26(12):860-862.
[2]李鹏.光学相干层析术理论与实验研究[D].南京理工大学,2010.
[3]杨军,计建军,李跃杰,王延群,刘亚伟,纪运红,宋学东.高频超声皮肤成像技术的研究[J].中国医疗器械杂志,2013,37(06):398-400.
[4]崔勇,林艳,于瑞星,王磊,冉玉平,刘洁,孙秋宁,李航,孟如松,邹先彪,郑亚杰,徐峰,章星琪,谢凤英.历史、现状和发展方略——皮肤镜在中国[J].中国皮肤性病学杂志,2017,31(02):119-122.
[5]肖佳,郭爱元,黄健,陈静.反射式共聚焦激光扫描显微镜在皮肤科的应用[J].中国麻风皮肤病杂志,2016,32(09):567-569.
[6]刘尚建,余菲,李凯,周静.太赫兹光谱与成像在生物医学领域中的应用[J].物理,2013,42(11):788-793.
[7] Brown, E.R., Taylor, Z.D., Tewari, P., Singh,R.S., Culjat, M.O., Bennett, D.B., Grundfest, W.S..THz imaging of skin tissue-Exploiting the strong reflectivity of liquid water[P]. Infrared Millimeter and Terahertz Waves(IRMMW-THz), 2010 35th International Conference on,2010.
[8]席鹏,刘宇嘉,姚志荣,任秋实.用于皮肤影像诊断的光学成像方法[J].中国激光,2011,38(02):7-19
[9]郑亚杰,沈雪,崔勇.皮肤镜联合反射式共聚焦显微镜对色素痣良恶性、脂溢性角化病的诊断价值[J].中日友好医院学报,2018,32(02):79-82+64.
[10]何桂录,曾小君,王海东,等.神经导航下术中超声联合荧光素钠在脑恶性肿瘤手术中的应用[J].中华神经创伤外科电子杂志,2018,4(04):231-234.
[11]吴振宇.荧光显像联合超声引导行乳腺癌前哨淋巴结活检的临床研究[D].河北医科大学,2016.
[12]周璟.高频超声的常见皮肤肿瘤表现及在基底细胞癌术前评估的应用研究[D].天津医科大学,2016.
[13]苏云伟.术前使用5-氨基酮戊酸提高Bowen病、鳞癌精准切除的临床研究[D].北京协和医学院,2015.
[14] Emily T, Jeffrey P, William Hanke.Photodynamic diagnosis of tumor margins using methyl aminolevulinate before Mohs micrographic surgery[J]. Journal of the American Academy of Dermatology, 2010(5):911-918.
[15] S.H.Ibbotson, An overview of topical photodynamic therapy in dermatology, Photodiagnosis Photodyn.Ther.7(Septembe 2009)(2010)16-23
[2]李鹏.光学相干层析术理论与实验研究[D].南京理工大学,2010.
[3]杨军,计建军,李跃杰,王延群,刘亚伟,纪运红,宋学东.高频超声皮肤成像技术的研究[J].中国医疗器械杂志,2013,37(06):398-400.
[4]崔勇,林艳,于瑞星,王磊,冉玉平,刘洁,孙秋宁,李航,孟如松,邹先彪,郑亚杰,徐峰,章星琪,谢凤英.历史、现状和发展方略——皮肤镜在中国[J].中国皮肤性病学杂志,2017,31(02):119-122.
[5]肖佳,郭爱元,黄健,陈静.反射式共聚焦激光扫描显微镜在皮肤科的应用[J].中国麻风皮肤病杂志,2016,32(09):567-569.
[6]刘尚建,余菲,李凯,周静.太赫兹光谱与成像在生物医学领域中的应用[J].物理,2013,42(11):788-793.
[7] Brown, E.R., Taylor, Z.D., Tewari, P., Singh,R.S., Culjat, M.O., Bennett, D.B., Grundfest, W.S..THz imaging of skin tissue-Exploiting the strong reflectivity of liquid water[P]. Infrared Millimeter and Terahertz Waves(IRMMW-THz), 2010 35th International Conference on,2010.
[8]席鹏,刘宇嘉,姚志荣,任秋实.用于皮肤影像诊断的光学成像方法[J].中国激光,2011,38(02):7-19
[9]郑亚杰,沈雪,崔勇.皮肤镜联合反射式共聚焦显微镜对色素痣良恶性、脂溢性角化病的诊断价值[J].中日友好医院学报,2018,32(02):79-82+64.
[10]何桂录,曾小君,王海东,等.神经导航下术中超声联合荧光素钠在脑恶性肿瘤手术中的应用[J].中华神经创伤外科电子杂志,2018,4(04):231-234.
[11]吴振宇.荧光显像联合超声引导行乳腺癌前哨淋巴结活检的临床研究[D].河北医科大学,2016.
[12]周璟.高频超声的常见皮肤肿瘤表现及在基底细胞癌术前评估的应用研究[D].天津医科大学,2016.
[13]苏云伟.术前使用5-氨基酮戊酸提高Bowen病、鳞癌精准切除的临床研究[D].北京协和医学院,2015.
[14] Emily T, Jeffrey P, William Hanke.Photodynamic diagnosis of tumor margins using methyl aminolevulinate before Mohs micrographic surgery[J]. Journal of the American Academy of Dermatology, 2010(5):911-918.
[15] S.H.Ibbotson, An overview of topical photodynamic therapy in dermatology, Photodiagnosis Photodyn.Ther.7(Septembe 2009)(2010)16-23