基于G1-熵权法和响应面设计优化益气活血方醇提工艺
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摘要
目的优化益气活血方醇提工艺参数。方法采用G1-熵权法和响应面设计,在单因素试验基础上,以乙醇体积分数、乙醇用量、提取时间为考察因素,黄芪甲苷和丹参酮类成分的转移率及浸膏得率为评价指标,优选益气活血方醇提最佳工艺参数。结果最佳醇提工艺参数为:用70%乙醇提取2次,第1次用8倍量乙醇提取120 min,第2次用6倍量乙醇提取90 min。3次平行验证试验平均综合评分为95.79,RSD=0.74%。结论优选出的益气活血方醇提最佳工艺稳定可行,可为益气活血方的新药开发提供依据。
Objective To optimize the technological parameters of ethanol extraction of Yiqi Huoxue Prescription.Methods Using G1-entropy method and response surface design, based on the single factor experiment, the volume fraction of ethanol, the amount of ethanol and the extraction time were taken as the evaluation factors. Extract yield and the transfer rate of astragaloside Ⅳ and tanshinones were evaluation indexes. The technological parameters of ethanol extraction of Yiqi Huoxue Prescription was optimized. Results The optimal technological parameters of ethanol extraction were extracted twice with 70% ethanol; the first ethanol dosage was 8 times, the extraction time was 120 min; the second ethanol dosage was 6 times, and the extraction time was 90 min. The average score of the three parallel validation tests was 95.79 and the RSD was 0.74%. Conclusion The optimal process of ethanol extraction of Yiqi Huoxue Prescription is stable and feasible, which can provide references for development of new medicine of Yiqi Huoxue Prescription.
Objective To optimize the technological parameters of ethanol extraction of Yiqi Huoxue Prescription.Methods Using G1-entropy method and response surface design, based on the single factor experiment, the volume fraction of ethanol, the amount of ethanol and the extraction time were taken as the evaluation factors. Extract yield and the transfer rate of astragaloside Ⅳ and tanshinones were evaluation indexes. The technological parameters of ethanol extraction of Yiqi Huoxue Prescription was optimized. Results The optimal technological parameters of ethanol extraction were extracted twice with 70% ethanol; the first ethanol dosage was 8 times, the extraction time was 120 min; the second ethanol dosage was 6 times, and the extraction time was 90 min. The average score of the three parallel validation tests was 95.79 and the RSD was 0.74%. Conclusion The optimal process of ethanol extraction of Yiqi Huoxue Prescription is stable and feasible, which can provide references for development of new medicine of Yiqi Huoxue Prescription.
引文
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[2]郭慧芳.郭志华教授运用益气活血方治疗心衰经验介绍[J].中医药导报,2014,20(7):151.
[3]唐斌,张金国,谭洪勇,等.黄芪甲苷对慢性心衰大鼠心肌纤维化及能量代谢的影响[J].中国病理生理杂志,2017,33(3):411-416.
[4]武梦羚,宋耀鸿.黄芪甲苷对异丙肾上腺素诱导的大鼠原代心肌细胞损伤的抗凋亡作用[J].中国实验方剂学杂志,2018,24(10):137-143.
[5]CHENGS,YUP,YANGL,etal.AstragalosideⅣenhances cardioprotectionofremoteischemicconditioningafteracute myocardial infarction in rats[J]. Am J Transl Res,2016,8(11):4657-4669.
[6]陈芬燕,郭韧,张毕奎.丹参酮ⅡA的心血管药理作用研究进展[J].中国中药杂志,2015,40(9):1649-1653.
[7]余良主,石春蓉.丹参酮ⅡA对高血压大鼠左心室肌肾素-血管紧张素系统不同成分表达量的影响[J].中国中药杂志,2014,39(8):1468-1472.
[8]王继龙,魏舒畅,刘永琦,等.基于G1-熵权法和正交设计优选黄芪百合颗粒的提取纯化工艺[J].中草药,2018,49(3):596-603.
[9]何晨阳,刘明月,时振伟,等.响应面法优化蛇莓花色苷浸提工艺及稳定性研究[J].中草药,2018,49(8):1829-1834.
[10]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:76,302.
[11]王丽.高效液相色谱法测定玉屏风颗粒中黄芪甲苷含量[J].天津药学,2018,30(3):20-21.
[12]罗堃,王元清,谢瑜,等.HPLC-ELSD法测定益气软皮丸中黄芪甲苷含量[J].亚太传统医药,2014,10(21):2-4.
[13]傅为忠,徐丽君.区域工业绿色发展成熟度动态评价——基于熵值修正G1法和距离协调度改进模型的实证分析[J].工业技术经济,2018,37(3):61-69.
[14]田彦芳,万海同,朱紫烨,等.基于熵权法的多目标筛选甘草黄酮类成分纯化工艺[J].中草药,2016,47(7):1118-1125.
[15]邹龙,张依人,张可人,等.补阳还五汤水提工艺的优化[J].中成药,2018,40(4):962-966.
[16]朱紫烨,田彦芳,张迁,等.BP神经网络结合熵权法优化甘草皂苷提取工艺[J].中成药,2017,39(9):1830-1834.
[17]叶利,田晓霞,尚凡,等.基于G1-熵权法的供电服务满意度指标权重组合优化方法[J].湖北电力,2016,40(9):45-50.
[18]李广胜,覃芳,杨季菱,等.HPLC法测定当归补血配方颗粒中黄芪甲苷的含量[J].中国药房,2015,26(36):5131-5133.