益肾活血方提取工艺研究
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摘要
目的确定益肾活血方的最优提取工艺。方法以水提出膏率和醇浸出物为指标,通过单因素分析方法筛选影响因素的最优水平,然后通过正交试验的方法,优化益肾活血方的最优提取工艺。结果单因素实验结果表明提取时间,加水量10倍和14倍时益肾活血方的出膏率和醇浸出物影响较大。经正交试验分析,确定益肾活血方的最佳提取工艺为:加水量12倍,提取次数3次,提取时间1 h。结论本实验优化的最佳提取工艺实验结果稳定,操作可行性强,可为该处方进一步开发利用提供技术参考。
Objective To determine the optimum extraction process of Yishen Huoxue Fang. Methods Using single factor and orthogonal test design to optimize the extraction process, with paste-forming rate and alcohol extract of Yishen Huoxue Fang as indexes. Results The results of single factor experiment showed that the extracting time, 12-fold water and 14-fold water have a great impact on paste-forming rate and alcohol extract of Yishen Huoxue Fang. Analysis of orthogonal test show that the optimal extraction process is adding 12-fold water, and extracting for 3 times, 1 h each time. Conclusion The optimized method is stable and has strong operational feasibility, and can provide reference for further development and utilization of the formula.
Objective To determine the optimum extraction process of Yishen Huoxue Fang. Methods Using single factor and orthogonal test design to optimize the extraction process, with paste-forming rate and alcohol extract of Yishen Huoxue Fang as indexes. Results The results of single factor experiment showed that the extracting time, 12-fold water and 14-fold water have a great impact on paste-forming rate and alcohol extract of Yishen Huoxue Fang. Analysis of orthogonal test show that the optimal extraction process is adding 12-fold water, and extracting for 3 times, 1 h each time. Conclusion The optimized method is stable and has strong operational feasibility, and can provide reference for further development and utilization of the formula.
引文
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[2]魏军平,高嘉良.益气活血法治疗糖尿病肾病的临床研究进展[J].世界中西医结合杂志,2014,9(4):446-448.
[3]李哲.益气活血方药配伍治疗冠心病疗效及作用机制的系统性研究[D].西安:第四军医大学,2015.
[4]邵莹,郑连冬,张丽.益气活血法治疗冠心病心绞痛临床观察[J].辽宁中医杂志,2007,15(4):468.
[5]余念星.自拟益气活血汤治疗老年冠心病心绞痛临床研究[J].亚太传统医药,2017,13(3):114-115.
[6]苏玉亭,杨宇.益气活血方治疗类风湿性关节炎疗效观察[J].中华中医药杂志,2012,27(6):1627-1628.
[7]张艳,刘晓蕾,王辰.益气活血法治疗慢性心力衰竭疗效评价[J].中国中医药信息杂志,2011,18(11):72-73.
[8]吕中,王宁,王东披.益气活血法对缺氧缺血性脑损伤保护作用的实验研究[J].中华中医药学刊,2011,29(7):1552-1555.
[9]龚丽萍.益气活血法预防高血压性脑卒中的临床与实验研究[D].广州:广州中医药大学,2014.
[10]杨顺智.益气化瘀法治疗颈动脉粥样硬化的临床效果[J].中国中医药现代远程教育,2017(1):56-58.
[11]王锋.益肾活血汤治疗慢性肾炎凝血功能的临床观察[J].内蒙古中医药,2017(4):7-8.
[12]陈春莺,钱芳,正交试验法优选首乌颗粒的水提醇沉工艺[J].现代中药研究与实践,2017,31(1):37-40.